显微外科技术在前臂血管神经损伤的早期应用

目的:探讨显微外科技术在前臂血管神经损伤早期应用的临床疗效。方法:采用显微外科技术对172例前臂血管神经损伤患者(神经损伤106例,血管损伤54例,血管合并神经损伤12例)进行早期修复;术后2~7 d采用血管造影对吻合血管进行评价;随访25~30个月,对患者神经修复情况进行评价。结果:神经损伤106例患者中,神经修复"优、良"者98例(优良率92.45%);血管损伤54例中,血管修复"优、良"者52例(优良率96.3%);血管合并神经损伤12例中,神经修复"优、良"者6例(优良率50.0%),血管修复"优、良"者6例(优良率50.0%)。本组神经修复总优良率为90.70%(156/172)。结论:早期运用显微外科技术治疗前臂血管神经损伤疗效显著。     

加味芍药甘草汤治疗混合痔术后疼痛的疗效及对患者血清SP、PGE2的影响

目的探讨加味芍药甘草汤治疗混合痔术后疼痛的临床疗效及对患者血清P物质(SP)、前列腺素E 2(PGE2)的影响。方法将2016年10月—2018年10月于本院肛肠科行混合痔手术的78例术后疼痛患者作为研究对象,采用随机数据表法进行分组。对照组39例予以常规治疗,观察组39例在此基础上予以加味芍药甘草汤。比较2组患者的血清SP、PGE 2水平,VAS疼痛评分,治疗总有效率。结果2组患者治疗后的血清SP、PGE2含量有一定的下降,观察组血清SP与PGE 2均低于对照组,差异有统计学意义(P<0.05);2组患者治疗过程中VAS评分呈不断降低的态势,观察组每个时间段的VAS评分均低于对照组,差异有统计学意义(P<0.05);观察组治疗总有效率为94.9%,对照组为82.1%,差异有统计学意义(P<0.05)。结论加味芍药甘草汤治疗混合痔术后疼痛的疗效满意,可明显改善患者术后的疼痛,对血清PS、PGE2水平产生有利的影响,缩短术后的康复时间,提高生活质量,值得一定的临床应用。     

室上速24例急诊治疗效果评价

以34例室上速患者为研究对象,随机均分为观察组和对照组各17例。在维持血压正常后,观察组采取可达龙治疗,对照组采取ATP治疗。比较两组患者临床疗效和治疗安全性。经治疗后,观察组总有效率为94.11%;对照组总有效率82.35%。观察组总有效率优于对照组,差异具有统计学意义(P<0.05)。观察组总安全率94.11%;对照组总安全率52.94%。观察组总安全率优于对照组,差异具有统计学意义(P<0.05)。可达龙在治疗室上速方面,能够快速治疗达到良好的治疗效果,而且在严重副作用和并发症方面,明显优于ATP的治疗效果,值得临床推广。     

丹参多酚酸改善心绞痛患者血小板功能临床观察

选取72例冠心病稳定性心绞痛患者为研究对象,随机均分为低剂量组、高剂量组和对照组。低剂量组采取丹参多酚酸200mg治疗,高剂量组采取丹参多酚酸400mg治疗。对照组采取普通丹参注射液治疗。采用比浊法测定血小板聚集率,比较临床疗效和血小板聚集率。结果低剂量组和高剂量组患者临床疗效差异无统计学意义(P>0.05);低剂量组和高剂量组显效率及总有效率高于对照组(P<0.05)。在5μmol/L ADP和300μmol/L ADP诱导剂下,低剂量组和高剂量组血小板凝集率优于对照组(P<0.05)。丹参多酚酸治疗心绞痛临床疗效佳,能够有效抑制血小板聚集。     

三种消毒剂对体检中心物体表面消毒效果与使用成本的比较研究

目的对3种消毒剂在体检中心物体表面消毒的消毒效果及使用成本进行比较,为体检中心优选合适的物体表面消毒方式提供依据。方法分别使用I型复合双链季铵盐卫生湿巾、500mg/L含氯消毒剂、75%乙醇擦拭消毒健康体检中心采血台、彩超机和乳腺红外线诊断仪的表面,在消毒前、消毒后即刻、消毒后30min及1h分别对各区域进行棉拭子涂抹采样及培养。结果受检物体表面细菌菌落数采血台为(78.4±6.2)cfu/cm2,彩超机为(36.2±2.1)cfu/cm2,乳腺红外线诊断仪为(45.9±3.0)cfu/cm2。3种消毒剂消毒后各时间点采样菌落数与消毒前比较,差异有统计学意义(均P0.01)。I型复合双链季铵盐卫生湿巾消毒效果在消毒后即刻、消毒后30min及1h优于500mg/L含氯消毒剂、75%乙醇(P0.05,P0.01);每次消毒成本I型复合双链季铵盐卫生湿巾最低。结论 3种消毒剂对各监测表面均有良好的消毒效果,但I型复合双链季铵盐卫生湿巾的消毒持续效果优于其他两种,且使用成本低,使用方便、侵蚀性小,适用于体检中心物表消毒。     

Daidzein affects proliferation and apoptosis in non-small cell lung cancer cells:role of p53 signaling pathway北大核心CSCD

Aim To investigate the effects of daidzein（DD） on the proliferation and apoptosis of non-small cell lung cancer cells,with a focus on the possible role of the p53 signaling pathway in this regard. Methods CCK-8 method and flow cytometry were used to detect the effects of soy isoflavone crude extract and DD on the viability and apoptosis of HELF and H1299 cells. Gene microarray was used to detect the changes in gene expression after treatment of H1299 cells with DD. GSEA and differential analysis were used to screen the major pathways and key genes. RT-qPCR and Western blot were performed to verify the differences in mRNA and protein expression of key genes（p53 and CASP9） in the major pathways. After p53 inhibitor Pifithrin-α inhibited the expression of p53,the effect of DD on p53 mRNA and protein expression levels was examined,and the proliferative effect on H1299 cells was observed. Results Soy isoflavone crude extract and DD promoted proliferation and inhibited apoptosis of normal lung cells and inhibited proliferation and promoted apoptosis of lung cancer cells. p53 signaling pathway was significantly enriched in the DD-treated group（NES=1.78,P=0.000）,and the expressions of p53 and CASP9 genes were found to be significantly up-regulated in the treated group. Compared with the control group,mRNA expression of CASP9 and p53 significantly increased in both HELF and H1299 cells treated with DD（P<0.05）,and p53 protein expression also increased in HELF cells（P<0.05）. After inhibition of p53 expression,DD significantly increased the mRNA expression of p53 in H1299 and HELF cells（P<0.05） and also markedly increased the expression of p53 protein in H1299 cells（P<0.05）,and it was observed that DD inhibited the proliferation of lung cancer cells. Conclusions DD inhibits the proliferation and promotes the apoptosis of lung cancer H1299 cells,and the mechanism mainly involves the p53 signaling pathway. © 2023 Publication Centre of Anhui Medical University. All rights reserved.