短期胰岛素泵强化治疗对初发2型糖尿病血管内皮功能影响的临床观察

目的：利用彩超测定肱动脉血流介导的血管舒张功能(FMD)评价2型糖尿病患者经2 w胰岛素泵强化治疗后,血管内皮依赖性舒张功能改善情况。方法对34例初诊2型糖尿病患者进行2 w的胰岛素泵强化治疗,分析比较其治疗前后血糖、Homa-IR、血脂及FMD水平。结果胰岛素泵强化治疗后FPG、2 hPG、HbA1c、血脂水平下降,Homa-IR降低,FMD水平明显升高。结论初诊2型糖尿病患者,短期胰岛素泵强化治疗能迅速平稳控制血糖、降脂、减轻胰岛素抵抗,并能显著改善FMD。     

人卵泡抑素基因真核表达载体构建和体外表达

目的:体外构建携带人卵泡抑素基因(FS)cDNA的真核表达载体,并观察其在幼年叙利亚地鼠肾细胞(BHK21)中的表达。方法:从新鲜人卵泡抽提液细胞中提取RNA,应用RT-PCR方法扩增人FScDNA序列,克隆到pMD-18T载体中,构成pMD-FS重组克隆质粒。经测序鉴定后,双酶切得到人FS基因cDNA序列,插入到真核表达载体pEGFP-N1中,构成pEGFP-FS重组表达质粒。pEGFP-FS重组表达质粒经酶切和PCR鉴定,通过脂质体2000介导瞬时转染BHK21细胞,并检测绿色荧光蛋白(GFP)的荧光表达。结果:成功构建pMD-FS重组克隆质粒,FScDNA序列测序结果与GenBank中公布的序列(Genbank NM_013409.1)同源性为100%;成功构建pEGFP-FS重组表达质粒,将其转染BHK21细胞后,观察到绿色荧光蛋白的表达。结论:本实验成功构建了人FS的pMD-FS重组克隆质粒和pEGFP-FS重组表达质粒,为进一步研究FS对哺乳动物卵泡发育的影响奠定了基础。     

中医药联合亚低温脑保护治疗的研究进展

本文通过文献分析探讨中医药联合亚低温脑保护治疗的疗效及应用前景。回顾亚低温脑保护治疗的作用机制及临床研究现状,并对近十年来国内有关中医药联合亚低温脑保护治疗的实验研究及临床研究文献作出分析。动物实验表明,适当的中医药联合亚低温脑保护治疗能更好地起到脑神经保护作用;但临床研究文献报道并不多见,需要经过大量、科学的研究和评估以进一步证实中医药联合亚低温脑保护治疗的临床疗效。中医药联合亚低温脑保护治疗的应用,目前属于探索阶段,为临床治疗脑血管病提供了一个新的思路,两者的联合应用具有广阔的前景。     

岭南林夏泉流派擅用虫类药治疗癫痫经验

林夏泉老中医认为癫痫病机本质在于肝、脾、肾脏气失和,风、痰、瘀、虚交错为患,自创经验方除痫散,运用虫类药物疏逐搜剔、灵动之性治疗难治性癫痫,临床取得很好疗效,该流派代表性医家刘茂才教授在林老除痫散基础上,制成益脑安胶囊,临床治疗癫痫,收效满意,体现了该流派运用虫类药息风、涤痰、化瘀、定痫的特色。     

Parkin基因启动子区-258T/G多态性与广西地区散发性帕金森病的相关性分析

Objective To investigate the association between the-258T/G polymorphism.in the pro-moter of parkin gene and the risk for sporadic parkinson's disease (SPD) in Guangxi Province, and in relationto the age of onset, of PD patients. Methods PCR-RFLP and sequence analysis were used to determine thegenotype of-258T/G polymorphism between all patients and healthy controls. Results The G allele was morecommon in patients than controls (55.20%:43.33% ,x2=6.898, P<0.05, OR=1.61, 95% CI: 1.13 ～2.30). The frequency of GG genotype was higher in patients than in controls (28.00 %: 18.33%, x2=7.159, P<0.05, OR=2.75, 95% CI : 1.31 ～ 5.77). The frequency of TG + GG genotype was higher in pa-tients than in controls (82.40%:68.33%, x2=6.551, P<0.05, OR=2.17.95%CI: 1.20 ～3.93). Afterbeing stratified by onset age, the frequencies of the G allele and GG genotype were significantly higher in pa-tients with onset age over 50 years than those in controls respectively. On the other hand, the frequency was notsignificantly different between the younger onset PD patients and the controls. Conclusion The parkin promot-er-258T/G polymorphism might be a risk factor for PD in Guangxi Province, and the G allele was increasedwith increasing age.     

不同b值扩散加权MRI诊断肝脏局灶性病变的初步研究

目的:探讨不同b值磁共振扩散加权成像(DWI)系列分析及表观扩散系数(ADC)值对肝脏常见局灶性病变定性诊断的价值.方法:对45例肝脏常见局灶性病变(15例肝癌,18例肝血管瘤,12例肝囊肿)行不同b值DWI (b=600 s/mm2,1000 s/mm2,2000 s/mm2),观察病灶DWI信号强度连续变化并计测表观扩散系数(ADC)值.结果:肝癌不同b值DWI均表现为高信号,随b值增加,病灶信号强度无显著性改变(P＞0.05);肝血管瘤DWI (b=600 s/mm2)表现为高信号,DWI (b=1000 s/mm2,2000 s/mm2)表现为高或等信号,随b值增加,病灶信号强度降低(P＜0.05);肝囊肿DWI (b=600)表现为高、等或低信号,DWI (b=1000 s/mm2,2000 s/mm2)表现为低信号,随b值增加,囊肿信号强度降低(P＜0.05).随b值增加,肝癌、肝血管瘤、肝囊肿的ADC值均逐渐降低(P＜0.05).结论:不同b值DWI系列分析可能鉴别诊断肝脏常见局灶性病变.     

中药“脑瘤消”治疗颅内恶性肿瘤疗效初步报道

神经胶质瘤扣脑转移瘤发病占颅内肿瘤一半以上。且恶性程度很高。本文初步报道采用中药脑瘤消治疗神经胶质瘤7例,脑转移瘤3例,其有效率为60%。     

Parkin基因启动子区-258T/G多态性与广西地区散发性帕金森病的相关性分析

Objective To investigate the association between the-258T/G polymorphism.in the pro-moter of parkin gene and the risk for sporadic parkinson's disease (SPD) in Guangxi Province, and in relationto the age of onset, of PD patients. Methods PCR-RFLP and sequence analysis were used to determine thegenotype of-258T/G polymorphism between all patients and healthy controls. Results The G allele was morecommon in patients than controls (55.20%:43.33% ,x2=6.898, P<0.05, OR=1.61, 95% CI: 1.13 ～2.30). The frequency of GG genotype was higher in patients than in controls (28.00 %: 18.33%, x2=7.159, P<0.05, OR=2.75, 95% CI : 1.31 ～ 5.77). The frequency of TG + GG genotype was higher in pa-tients than in controls (82.40%:68.33%, x2=6.551, P<0.05, OR=2.17.95%CI: 1.20 ～3.93). Afterbeing stratified by onset age, the frequencies of the G allele and GG genotype were significantly higher in pa-tients with onset age over 50 years than those in controls respectively. On the other hand, the frequency was notsignificantly different between the younger onset PD patients and the controls. Conclusion The parkin promot-er-258T/G polymorphism might be a risk factor for PD in Guangxi Province, and the G allele was increasedwith increasing age.     

199例头风病患者证候特点及与血小板5-羟色胺关系研究

目的：对头风病患者证候分布、临床特点及与血小板5-羟色胺关系进行分析。方法：199例头风病患者分为风火候、风痰候、风瘀候、正虚候,观察各证候间年龄、头痛病程、头痛指数差异及与血小板5-羟色胺关系,及头风病各症候与风、火、痰、瘀、郁、气虚、血虚各致病因素之间的相关性。结果：各证候间病程、头痛程度、持续时间比较,差异有非常显著性意义（P〈0.01）,各证候间血小板5-羟色胺浓度比较,差异也有显著性意义（P〈0.05）。风火候易于与致病因素郁相夹杂致病（P〈0.01）;风痰候易于与致病因素瘀和气虚相夹杂致病（P〈0.01）;正虚候易于与致病因素痰相夹杂致病（P〈0.05）;风瘀候易于与致病因素痰和血虚相夹杂致病（P〈0.05）。结论：风火候是头风病最常见的证候类型,头风病各证候间血小板5-羟色胺浓度有显著差异。     

七种常见疾病不同性别对血液流变性的影响

本文所观察的七种常见病,即冠心病、缺血性中风、高血压病、周围血管病、肺心病、慢性肾病及糖尿病。目前普遍认为这些疾病的病理变化与中医“瘀血”相关,大量文献报道。这些疾病都存在着不同程度的血液流变学指标的改变。我们从1989年—1991年共观察患上述疾病的190例患者及健康对照组60例,男女性别的血液流变学差异,发现其血液流变学指标有着各自的特征性表现,现报告如下: