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排序方式: 共有233条查询结果,搜索用时 15 毫秒
1.
目的观察翼状胬肉切除联合自体角膜缘干细胞移植术治疗翼状胬肉的疗效。方法采用翼状胬肉切除联合自体角膜缘干细胞移植术方法治疗翼状胬肉【】50例(52只眼)观察术后角膜情况进行术前术后对比,并随访半年。结果 50例(52只眼)手术中、术后无严重并发症,随访半年,只有1例复发。结论翼状胬肉联合自体角膜缘干细胞移植术,疗效满意,大大降低术后复发率。  相似文献   
2.
目的:探讨角巩膜缘切口在小切口向超声乳化白内障手术转型中的应用.方法:选取我院在2012-02/2013-02接受手术的200例200眼白内障患者,行角巩膜缘切口超声乳化手术,术中如遇不安全因素即改为SICS+IOL术,手术均顺利完成.结果:顺利实施超声乳化白内障手术者共172眼(86%),28眼(14%)改为角巩膜缘切口SICS+IOL术,术后视力≥0.5者176眼(88%),0.1~0.4者22眼(11%),0.1以下者2眼(1%).术后均无严重并发症.结论:在小切口转型超声乳化白内障手术初期时,运用角巩膜缘切口能提高手术的可操作性和安全性,是具有小切口白内障手术经验眼科医生很好的选择方式.  相似文献   
3.
PurposeLimbal niche cells (LNCs) play a vital role in the maintenance of limbal epithelial stem/progenitor cells (LESCs). Four methods have been reported to isolate and expand LNCs: digestion by collagenase alone (C-LNC), collagenase following dispase removal of the limbal epithelium (DC-LNC), dissection of dispase-isolated limbal epithelial sheets (D-LNC), and explant cultures of limbal stromal tissues (Ex-LNC). This study aimed to isolate LNCs using those four methods and to compare their capacity to maintain LESCs.MethodsLNCs were isolated from the rat corneal limbus by the following methods: C-LNC, DC-LNC, D-LNC, and Ex-LNC. Quantitative real-time PCR and immunofluorescence staining were used to analyze the expression of embryonic stem cell (ESC) markers. The ability to maintain LESCs was assessed on the basis of colony-forming capacity and the expression of progenitor, proliferation, and differentiation markers in three-dimensional (3D) Matrigel and Transwell systems. Notch signaling of LESCs supported by different LNCs in Transwell inserts was analyzed by quantitative real-time PCR.ResultsDC-LNCs exhibited lower expression of CK12 during isolation and expansion. Among P4-expanded LNCs, DC-LNCs expressed significantly higher levels of Sox2, Oct4, Nanog, and N-cadherin than C-LNCs, D-LNCs, and Ex-LNCs. Compared with other LNCs, DC-LNCs were more effective in maintaining LESCs with higher holoclone-forming efficiency, greater expression of ΔNp63α and Ki67, and lower expression of CK12. DC-LNCs were also more capable of downregulating Notch signaling of LESCs.ConclusionsDC-LNCs were more effective in expressing ESC markers and maintaining LESCs compared to other LNCs. This study identifies an optimal method for the isolation of LNCs in tissue engineering and ocular surface reconstruction.  相似文献   
4.
Purpose: Storage time for donor corneas in Optisol GS is limited compared to Eye Bank Organ Culture (EBOC). We here examine the epithelium on donor corneoscleral rims after primary storage in Optisol GS and subsequent incubation in EBOC. Methods: Morphology was monitored by light and electron microscopy, expression of phenotypic and genotypic markers by immunohistochemistry and RT‐PCR and changes in oxidative lipid and DNA damage by ELISA and COMET assay. Results: A prominent loss of cells was observed after storage in Optisol GS. After maintenance in EBOC, spreading apical cells were Occludin+, while the staining for E‐cadherin and Connexin‐43 was less intense. There were an upregulation of Occludin and a downregulation of E‐cadherin and Connexin‐43. Eye Bank Organ Culture was associated with an ongoing proliferative activity and a downregulation of putative progenitor/stem cell marker ABCG2 and p63. Staining for 8‐OHdG and Caspase‐3 did not increase, while levels of malondialdehyde and number of DNA strand breaks and oxidized bases increased. Conclusions: This dual procedure should be pursued as an option to increase the storage time and the pool of available donor corneas. The observed downregulation of markers associated with stemness during EBOC is relevant considering the potential use of donor epithelium in the treatment of ocular surface disorders.  相似文献   
5.
目的 比较翼状胬肉单纯切除术、翼状胬肉切除术联合游离结膜瓣移植术及翼状胬肉切除术联合角膜缘干细胞移植术对翼状胬肉的治疗效果.方法 将171例(186眼)翼状胬肉患者随机分为A、B、C3组,A组采用翼状胬肉单纯切除术,B组采用翼状胬肉切除联合游离结膜瓣移植术,C组采用翼状胬肉切除联合自体角膜缘干细胞移植术.比较各组间术后复发率、泪膜功能变化及术后并发症的发生率.结果 A组的治愈率明显低于B、C组,且并发症发生率、修复时间及复发率均明显高于B、C组,差异具有统计学意义(P<0.05).C组并发症发生率、复发率低于B组,修复时间和治愈率优于B组,具有显著性差异(P<0.05).B、C组术后早期的泪膜稳定性优于A组,差异具有统计学意义(P<0.05).结论 与单纯翼状胬肉切除术和翼状胬肉切除联合游离结膜瓣移植术比较,翼状胬肉切除联合自体角膜缘干细胞移植术治疗翼状胬肉并发症少、复发率低,而且能够改善泪膜功能,是治疗翼状胬肉有效的手术方式.  相似文献   
6.

Objective:

To describe the diagnosis and management of a competitive male basketball player with discogenic low back pain and presence of an old posterior ring apophyseal fracture (PRAF). This case will highlight the importance of early recognition and considerations regarding patient management for this differential of radiating low back pain.

Clinical Features:

A 21-year-old provincial basketball player presented with recurrent radiating low back pain into the left groin and lower limb. After several weeks of persistent symptoms including pain, muscle weakness, and changes in the Achilles deep tendon reflex, imaging was obtained that revealed a large disc extrusion with an old posterior ring apophyseal fracture. In collaboration with a spine surgeon and family physician, the patient was treated using a conservative, multimodal approach. Treatment consisted of graded mobilizations, spinal manipulative therapy, interferential current, and soft tissue therapy to the lumbar spine. Rehabilitation exercises focused on centralizing symptoms and improving strength, proprioception and function of the lower limb. After a period of 8 weeks, the patient was able to complete all activities of daily living without pain in addition to returning to basketball practice.

Summary:

PRAF is a unique condition in the immature spine and recent evidence suggests that those involved in sports requiring repetitive motion of the lumbar spine may be at increased risk. The astute clinician must consider this differential in young populations presenting with discogenic low back pain, as a timely diagnosis and necessary referral may allow for effective conservative management to reduce symptoms. Equally as important, one must be aware of the complications from PRAF as a contributing source of low back pain and dysfunction into adulthood. Knowing when to refer for advanced imaging and/or a surgical consult given the variable clinical presentation and prognosis is an essential component to care.  相似文献   
7.
目的:评价两种不同手术方法治疗翼状胬肉对泪膜稳定性的影响。方法选取翼状胬肉患者60例60眼随机分为A、B两组,各30例30眼,A组行翼状胬肉切除联合羊膜移植术,B组行翼状胬肉切除联合角膜缘干细胞移植术,比较两组间术前、术后泪液分泌试验(Schirmerl试验)、泪膜破裂时间(BUT)、泪液羊齿状物试验(TFT),评估手术对泪膜稳定性的影响。结果 Schirmerl试验:A组和B组术后与术前相比差异均无显著性(P>0.05),A组与B组比较差异无显著性(P>0.05)。BUT:A组和B组术后与术前相比差异均有显著性(P<0.05),术前1d、术后2周、术后4周数值A组与B组比较差异无显著性(P>0.05);术后12周B组BUT较A组改善明显,差异有显著性(P<0.05)。 TFT:A组和B组术后与术前相比差异均有显著性(P<0.05),A组与B组比较差异无显著性(P>0.05)。结论翼状胬肉切除联合角膜缘干细胞移植术与翼状胬肉切除联合羊膜移植术,两种手术方式术后泪膜稳定性较术前均有改善;两者相互比较,术后早期泪膜稳定性改善无明显差异,但3个月后前者对泪膜改善更佳。  相似文献   
8.
改良角膜缘干细胞移植术治疗翼状胬肉临床疗效观察   总被引:1,自引:1,他引:0  
陈义  金海霞  赵艳  胡善萌 《安徽医药》2013,34(7):882-884
目的探讨改良自体角膜缘干细胞移植术治疗翼状胬肉的临床疗效。方法将2012年5月至2013年3月收治的26例(34眼)原发性翼状胬肉患者随机分为两组,分别采用传统自体角膜缘干细胞移植术和改良自体角膜缘干细胞移植术,观察两组患者术后疼痛不适感、角膜上皮愈合情况及泪膜破裂时间(breaking-up time,BUT)。结果采用改良自体角膜缘干细胞移植术患者术后疼痛不适感明显减轻、角膜上皮愈合时间缩短及术后早期泪膜破裂时间延长。结论改良自体角膜缘干细胞移植术是一种能够减轻患者术后疼痛,缩短术后恢复时间的改良术式。  相似文献   
9.
Fibromodulin regulates collagen fibrillogenesis, but its existence/role(s) in the cornea is controversial. We hypothesize that fibromodulin regulates fibrillogenesis during postnatal development of the anterior eye. Fibromodulin is weakly expressed in the limbus at post‐natal day (P) 4, increases and extends into the central cornea at P14, becomes restricted to the limbus at P30, and decreases at P60. This differential spatial and temporal expression of fibromodulin is coordinated with emmetropization; the developmental increase in axial length and globe size. Genetic analysis demonstrated that fibromodulin regulates fibrillogenesis in a region‐specific manner. At the limbus, fibromodulin is dominant in regulating fibril growth during postnatal development. In the posterior peripheral cornea, cooperative interactions of fibromodulin and lumican regulate fibrillogenesis. These data indicate that fibromodulin plays important roles in the regulation of region‐specific fibrillogenesis required for the integration of the corneal and scleral matrices and sulcus development required for establishment of the visual axis. Developmental Dynamics 239:844–854, 2010. © 2010 Wiley‐Liss, Inc.  相似文献   
10.
目的:探讨下方角巩膜缘牵引缝线在眼科手术中的应用价值和操作技巧。方法:应用下方角巩膜缘牵引缝线方法固定眼球,暴露手术野协助完成眼科手术163例181眼,包括小切口白内障囊外摘出术联合人工晶状体植入术86例98眼,青光眼小梁切除术48例54眼,青光眼白内障联合手术10例10眼,结膜瓣遮盖手术8例8眼,玻璃体腔注气术3例3眼,外伤性睫状体脱离缝合复位手术2例2眼,经睫状体平坦部三通道玻璃体切除手术6例6眼。用直尺测量12∶00位角巩膜缘至上睑缘的距离(mm)。结果:应用下方角巩膜缘牵引缝线协助完成眼科手术163例181眼,操作效果满意者160例178眼,占98.3%。不满意者3例3眼,占1.7%,暴露不满意者为青光眼伴小睑裂患者行外眦开大术后达到满意效果,无1例发生并发症。测量12∶00位角巩膜缘至上睑缘的距离为9.5±3.5mm。结论:下方角巩膜缘牵引缝线在固定眼球和暴露手术野方面能够顺利协助完成眼科手术,具有操作简单,安全性高,实用性强的特点,效果满意,可以代替上直肌牵引缝线,值得临床眼科医生在手术操作中应用。  相似文献   
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