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岑令平  梁嘉健  张铭志 《眼科》2012,21(4):256-260
目的研究基质细胞衍生因子-1(SDF-1)在视网膜培养中促神经节细胞轴突再生的作用。设计实验研究。研究对象Fischer 344大鼠视网膜。方法视网膜培养之前5天夹断大鼠视神经;培养前用多聚赖氨酸及层黏连蛋白包被培养板;培养时,把分离出来的视网膜放射状剪成8小片,每小片视网膜粘铺于已包被好的培养板孔中,每5-6块取自不同大鼠的视网膜块随机组成一组,共7组,分别置于7种不同的Neurobasal-A/B27培养基中(对照组、20 ng/ml、70 ng/ml、200 ng/ml、500ng/ml及1000 ng/ml5个SDF-1浓度梯度处理组及SDF-1联合AMD3100阻断组)培养7天,在倒置显微镜下计数再生神经轴突的数量及长度,并对各组进行比较。主要指标再生神经轴突的数量及长度。结果对照组平均每个视网膜培养块的再生神经突数量及长度分别为17根及约1 mm;SDF-1促进轴突再生的作用随其浓度的增高而增强,中高浓度SDF-1组可使轴突再生增强2-3倍,而应用受体阻断剂AMD3100可使SDF-1的促再生作用减半。结论 SDF-1具有促进视网膜神经节细胞轴突再生的作用且具有浓度依赖性。  相似文献
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Purpose  To investigate whether electrical stimulation promoted axonal regeneration of retinal ganglion cells (RGCs) after optic nerve (ON) crush in adult rats. Methods  Transcorneal electrical stimulation (TES), which stimulates the retina with current from a corneal contact lens electrode, was used to stimulate the eye. TES was applied for 1 h immediately after ON crush. Axonal regeneration was determined by anterograde labeling of RGC axons. To examine whether the axonal regeneration was mediated by insulin-like growth factor 1 (IGF-1) receptors, an IGF-1 receptor antagonist, JB3, was injected intraperitoneally before each TES application. Immunostaining for IGF-1 was performed to examine the effects of TES. To test the survival-promoting effects of TES applied daily, the mean density of retrogradely labeled RGCs was determined on day 12 after ON crush. Results  Compared with sham stimulation, the mean number of regenerating axons significantly increased at 250 μm distal from the lesion and increased IGF-1 immunoreactivity was observed in retinas treated daily with TES. Preinjection of an IGF-1 receptor antagonist significantly blocked axonal regeneration by TES applied daily. TES applied daily also markedly enhanced the survival of RGCs 12 days after ON crush. Conclusion  TES applied daily promotes both axonal regeneration and survival of RGCs after ON crush.  相似文献
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赵亮  许家军 《国际眼科杂志》2011,11(10):1737-1739
作为一种特殊的钙调蛋白,癌钙蛋白(oncomodulin,OncoM,OCM)一直被认为只在肿瘤的发生、生长中起调节作用。然而近年来研究发现,OCM对视神经损伤具有修复功能,为神经损伤的治疗提供了更为广阔的视野。本文就目前OCM在视神经损伤修复中的作用及研究进展作一综述。  相似文献
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Background Neuroprotection is essential for repair processes after a traumatic insult in the central nervous system. We have demonstrated previously significant neuroprotective properties of the anti-apoptotic drug aurintricarboxylic acid in the model of axotomised retinal ganglion cells. Glucocorticoids are widely used to treat injuries of the nervous system. Due to the anti-inflammatory and microglia-inhibiting properties of glucocorticoids, we studied the neuroprotective effects of intravitreally administered cortisol after an optic nerve cut. Methods Ninety-eight adult Sprague–Dawley rats were used in this study. The optic nerve was cut intra-orbitally. Either vehicle or compound solution was injected intravitreally. Fluorescent dye was put onto the optic nerve stump to label retinal ganglion cells retrogradely. Retinal whole mounts were prepared 2 weeks after axotomy, and surviving retinal ganglion cells were counted. Results Two weeks after axotomy, up to 50±7% of all retinal ganglion cells survived if cortisol was injected into the eye compared with 17±5% survival if only vehicle solution was injected. The neuroprotective effects of aurintricarboxylic acid (43±5% survival) could be further enhanced if combined with cortisol (up to 61±5% survival). Regeneration of cut retinal ganglion cell axons into a peripheral nerve graft could also be enhanced by an intravitreal injection of cortisol (169±42 regenerating retinal ganglion cells per mm2 vs. 73±12 cells per mm2 after vehicle injection). The increase was not as high as with aurintricarboxylic acid (192±40 cells per mm2), although more retinal ganglion cells survived with cortisol. This indicates that neuronal survival alone is not sufficient for subsequent axonal regeneration. Nevertheless, regeneration could be markedly increased if aurintricarboxylic acid and cortisol were combined (308±72 cells per mm2). Conclusions Whereas aurintricarboxylic acid seems to act directly on lesioned retinal ganglion cells, cortisol seems to act on the glial environment, as indicated by microglial cell morphology and enhanced glial fibrillary acidic protein expression. The results show that both neuroprotection and regeneration can be enhanced by the combination of two simple compounds acting on different sites.  相似文献
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目的:观察嗅鞘细胞在成年大鼠视神经眶内断端移植的冻融周围神经中存活、生长与分布随存活时间的变化及对视神经再生的影响。方法:取成年大鼠坐骨神经反复冻融后,自两端注入10μL嗅鞘细胞悬液(105/μL),再移植于同种异体动物经眶内切断的视神经眶内断端上,分别于术后2d;1,2,3及4wk取移植物,切片观察嗅鞘细胞在其中的存活、生长及分布,并计数术后3,4wk动物视网膜内经移植物远端以50g/L荧光金逆行标记的再生视网膜节细胞。结果:嗅鞘细胞自注射部位增殖并迁移至周围神经全段,2wk时数量达到高峰,3,4wk时仅见神经周边部位残存极少量嗅鞘细胞,且视网膜内未见任何轴突再生的视网膜节细胞。结论:嗅鞘细胞可在冻融周围神经移植物中存活2 ̄3wk,神经周边部位较实质内更有利于嗅鞘细胞生长,这种移植物不能发挥促进视神经再生的作用。  相似文献
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视神经属于中枢神经的一部分,损伤后难以再生。视神经损伤通常伴随视网膜神经节细胞(retinal ganglioncells,RGCs)的持续性凋亡及视神经变性坏死,引起视力损害甚至完全失明。目前针对视神经再生的基础研究主要集中于保护和维持视神经损伤后RGCs的存活、促进RGCs轴突再生及重建视神经功能。本文以RGCs保护、轴突再生及视神经功能重建等为关键词,查询国内外最新视神经再生研究类文献,并分析整理,从抗氧化应激、提供外源性细胞因子、炎症刺激、抗胶质瘢痕、基因调控等方面阐述近年的视神经再生研究进展,以期对后续的基础研究开展及临床转化有所帮助。  相似文献
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