小包装全氟丙烷气体动力学实验研究

为检验塑料小包装全氟丙烷气体（C3F8）在不同包装和储存方法时浓度变化，将装有5～7mlC3F8的聚氯乙烯小袋，根据不同储藏温度和外包装方法随机分成四组：（1）22℃聚乙烯外包装，（2）36℃聚乙烯外包装，（3）22℃铝箔真空外包装，（4）-29℃聚乙烯外包装；每一组C3F8小袋气体存放一定时间后，应用气相色谱分析方法进行浓度测量。结果：第3组C3F8浓度最高和稳定，第2组浓度随放置时间降低最明显，第4组是临床应用气体的储藏和包装方法，其30天样本浓度和第3组相等，但放置一年时浓度降低。结果显示：塑料小包装C3F8予以铝箔真空外包装是一种可行的方法，利于C3F8运输和普及；聚乙烯外包装的C3F8，应放在-29℃保存，时间不超过一年。     

血清保存方法对促甲状腺激素结果的影响观察

目的 :观察血清保存方法对促甲状腺激素 (TSH)结果的影响。方法 :将甲状腺功能正常、异常患者共 10份血清 ,采用不同血清量、不同条件分组保存后测定 TSH。结果 :保存方法对 TSH结果有一定的影响。结论 :用于 TSH血清的保存量要多、要密封管口、低温保存。     

The adult and a new late adult forms of neuronal ceroid lipofuscinosis

Summary Three cases of the late adult form of neuronal ceroid lipofuscinosis (NCL) are reported. Two of these are siblings with a late clinical onset at ages 26 and 44 years. The third case, sporadic, has the oldest reported age for the onset of NCL, at 63 years and may be regarded as the first example of the presenile form of NCL. The clinical, morphological, histochemical, ultrastructural and genetic features of these three cases are discussed. The literature of the clinicopathological NCL cases with an onset at age of 25 and older is reviewed. The clinical and morphological differences between the late adult form and the presenile form of NCL as well as the difficulties in making the diagnosis are discussed.Supported by NIH Grant NS23717     

Synergistic activity of vitamin-C and vitamin-E to ameliorate the efficacy of stored erythrocytes

ObjectivesErythrocytes are exposed to oxidative stress during storage and can be stored for up to 42 days (in AS-7) under blood bank conditions for transfusion. Vitamin-C and Vitamin-E have proved beneficial in diminishing oxidative stress. Therefore, this study aims to investigate the combined effects of Vitamin-C and Vitamin-E on erythrocytes during storage.Materials and methodsBlood was collected from male Wistar rats and erythrocytes were isolated and stored in AS-7 (Additive Solution) at 4 °C for 35 days. Erythrocytes were grouped into i) Controls and ii) Experimentals [Vitamin-C (10 mM) and Vitamin-E (2 mM)]. Antioxidant and oxidative stress markers were assessed at weekly intervals. Statistical analyses were performed by using GraphPad Prism software.ResultsHemoglobin increased on days 7 and 14 in the Experimentals. Superoxide dismutase activity elevated on days 7 & 14 in Controls and on day 7 in Experimentals. Catalase activity increased on day 21 in both groups. Protein carbonyls decreased on days 21 and 28 in Experimentals. Thiobarbituric acid reactive substances decreased from day 14 in both groups. Conjugate dienes decreased on days 21 & 35 in the Experimentals. Glutathione increased from day 14 in both groups. Superoxides decreased on days 14, 28 & 35 in Controls and from day 14 in Experimentals.ConclusionVitamin-C and Vitamin-E have been beneficial in terms of hemoglobin, antioxidants, protein & lipid oxidations and superoxides in stored erythrocytes. Therefore, this study provides new avenues for the development of effective storage solutions which will have a clinical impact in erythrocyte transfusions.     

Effects of scopolamine on variable intertrial interval spatial alternation and memory in the rat

A repeated measures procedure, variable intertrial interval (ITI) spatial alternation, was used to assess scopolamine effects on memory, and to compare effects of the drug on discrimination processes with effects on storage. Rats learned in two stages to press left and right levers in alternation on discrete trials separated by 5 different ITI's ranging from 2.5 to 40 s and presented in random order during the experimental session. In the first stage, alternating discrimination, alternation was controlled by a light on over the correct lever at the time of the trial; in the second stage, variable ITI spatial alternation, a centrally located panel light signalled all trials and alternation was controlled by stimuli from prior trials (memory). Alternation response occurrence declined moderately (but significantly) with increasing ITI duration in both the alternating discrimination and variable ITI spatial alternation stages; response occurrence was also significantly decreased by scopolamine treatment in both stages. Accuracy of alternating discrimination performance was not significantly altered by either ITI duration or scopolamine treatment. Accuracy of variable ITI spatial alternation performance on a trial varied inversely with the duration of the ITI that preceded the trial. Scopolamine treatment significantly reduced accuracy of lever pressing in variable ITI spatial alternation but did not alter the slope of the curves relating accuracy to ITI duration. These effects indicate that the drug impaired discrimination processes but did not alter memory storage.     

基于IP的医院信息系统存储策略

分析了医院存储系统特点，在比较现有各种存储技术的基础上，提出了基于IP的网络存储策略。     

Aging of platelets stored for transfusion

A goal of platelet storage is to maintain the quality of platelets from the point of donation to the point of transfusion – to suspend the aging process. This effort is judged by clinical and laboratory measures with varying degrees of success. Recent work gives encouragement that platelets can be maintained ex vivo beyond the current 5 -7 day shelf life whilst maintaining their quality, as measured by posttransfusion recovery and survival. However, additional measures are needed to validate the development of technologies that may further reduce the aging of stored platelets, or enhance their hemostatic properties.     

Physical and chemical changes in Alhydrogel™ damaged by freezing

Accidental freezing of aluminum-based vaccines occurs during their storage and transportation, in both developed and developing countries. Freezing damages the freeze-sensitive aluminum adjuvanted vaccines, through separation of lattice between aluminum adjuvant and antigen, leading to formation of aluminum aggregates, and loss of potency. In this study, we examined Alhydrogel™ ([AlO(OH)]xnH₂O, aluminum hydroxide, hydrated for adsorption) stored under recommended conditions, and exposed to freezing temperature until solid-frozen. The main purpose of our research was to determine the destruction areas of the solid-frozen Alhydrogel™ using selected methods of scanning electron microscopy, energy dispersive X-ray spectroscopy, Raman spectroscopy, Fourier-transform infrared spectroscopy and transmission electron microscopy working in diffraction mode. The Zeta potential evaluation, measurements of albumin adsorption power, thermogravimetric analysis and estimation of the mass loss after drying indicated significant structural (physical) and chemical differences between the freeze-damaged and non-frozen vaccine adjuvant. The presented results are important to better understand the type and nature of damages occurring in freeze-damaged aluminum-based vaccines. These results can be used in future studies to improve the temperature stability of aluminum adjuvanted vaccines.