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1.
三种蜱媒传染病在媒介蜱和鼠类中复合感染的研究   总被引:8,自引:0,他引:8  
目的 了解中国部分地区埃立克体病和其他蜱媒传染病病原体的复合感染情况。方法 运用聚合酶链反应方法对内蒙古自治区、黑龙江省、北京市的蜱和鼠类标本粒细胞埃立克体、莱姆病螺旋体、斑点热群立克次体的感染进行检测,对福建省的蜱和鼠脾脏标本中查菲埃立克体、斑点热立克次体及莱姆病螺旋体的感染情况进行检测。结果 内蒙古采集的全沟硬蜱408只,人粒细胞埃立克体感染率68%,莱姆病螺旋体感染率7.8%,斑点热群立克次体感染率45.6%,其中粒细胞埃立克体与莱姆病螺旋体复合感染标本5份(5/408),粒细胞埃立克体与斑点热群立克次体复合感染标本1份(1/408)。黑龙江采集的46只全沟硬蜱中人粒细胞埃立克体感染率6.5%,莱姆病螺旋体感染率10.8%,斑点热群立克次体感染率34.8%,其中粒细胞埃立克体与莱姆病螺旋体复合感染标本1份(1/46)。北京市采集的蜱类标本922只,有2只检测到莱姆病螺旋体的DNA片段。福建省宁化地区采集的优势蜱种越原血蜱283组(659只)中,查菲埃立克体最小阳性率为3.8%(25组阳性),38份野鼠脾脏的阳性率为564%(21只阳性),检测出有2只(2/659)蜱同时感染了粒细胞埃立克体和斑点热群立克次体,野鼠的脾脏标本中,也发现有一只同时感染了查菲埃立克体和斑点热立克次体。结论 人粒细胞埃立克体和莱姆病螺旋体复合感染以及查菲埃立克体和斑点热群立克次体复合感染可能在特定地区发生;其地区的蜱类、鼠类存在着极低的复合感染。  相似文献
2.
东北部分地区蜱传埃立克体DNA的检测   总被引:8,自引:0,他引:8  
目的 了解东北部分地区一些蜱种中是否携带埃立克体。方法 应用16SrRNA基因构建的特异性引物进行半套式PCR,检测蜱标本中埃立克体DNA,并对扩增产物进行克隆和序列测定,与基因库中已知序列进行同源性比较。结果从辽宁清原和吉林抚松、和龙、敦化、珲春的全沟硬蜱(Lpersuleatus)和森林革蜱(D.silvarum)中均扩增出了查菲埃立克体DNA,扩增片段与美国分离株(基因库M73222)相对应片段完全一致,同源性为100%;另外从吉林抚松、珲春的全沟硬蜱中扩增出了粒细胞埃立克体DNA,扩增片段与美国分离株(基因库U02521)相对应片段相差2个碱基,同源性为99.7%。结论 发现东北部分地区存在埃立克体病的病原学迹象,提示该地区可能存在埃立克体病的自然疫源地。  相似文献
3.
应用半巢式PCR检测我国北方一些蜱种中的查菲埃立克体   总被引:1,自引:0,他引:1  
目的 :了解我国北方一些蜱种中是否携带查菲埃立克体 (Ehrlichiachaffeensis,简写为EC)。方法 :应用半巢式PCR检测蜱的带菌率 ,利用 16SrRNA基因测序方法鉴定所测序列。结果 :从内蒙古莫尔道嘎林业局采集的全沟硬蜱和森林革蜱中扩增出查菲埃立克体的 16SrRNA基因 ,阳性率分别是 39.0 6 %和 10 .0 0 % ;并从新疆精河采集的全沟硬蜱和草原革蜱中也测到相同的序列 ,最小阳性率分别为 5.79%和 1.6 7%。对莫尔道嘎采集的蜱标本进行 12 2 0bp的EC16SrRNA基因分析 ,结果与美国 1株查菲埃立克体 (GenBankU2 350 3)的相应序列只差 1个碱基。结论 :所使用的两套半巢式PCR扩增引物 ,可以简便快速地检测和分析蜱标本中可能存在的EC  相似文献
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「目的」建立一个快速、简便的检测埃立克体感染的实验方法,用于人类埃立克体病的临床诊断及分子流行病学调查。「方法」用查菲埃立克体分离株(91HE17)感染犬巨噬细胞(DH82细胞),感染40天后收集DH82细胞。根据查菲埃立克体16SrRNA基因序列设计引物,特生扩增查菲埃立克体DNA。运用限制性片段长度多态性分析对PCR扩增产物进行鉴定。「结果」扩增产物经过1.5%琼脂糖凝胶电泳和5%聚丙烯酰胺凝  相似文献
6.
The prevalence of antibodies reactive withRickettsia conorii, Rickettsia typhi, Coxiella burnetii andEhrlichia chaffeensis was investigated using indirect immunofluorescence (IFA) test on human sera obtained from 300 blood donors in Casablanca and 126 sera obtained from clinical laboratories in Fez. In sera from Casablanca, antibodies reactive at titers >=1: 32 were found againstR. conorii (7%), andR. typhi (1.7%), but not againstE. chaffeensis. In the sera from Fez, antibodies were also detected againstR. conorii (5.6%),R. typhi (4%), but not againstE. chaffeensis. By Western immunoblotting, seroprevalence forR. conorii was in Casablanca and 4.8% in Fez. Antibodies reactive at titers >=1:50 againstC. burnetii (phase II) were present in sera from Casablanca (1%) and Fez (18.3%).Abbreviations IFA Immunofluorescence assay - MSF Mediterranean spotted fever - PBS Phosphate-buffered saline  相似文献
7.
Human ehrlichiosis is a recently recognized rickettsial disease. It is caused byEhrlichia chaffeensis, an intraleucocytic Gram-negative, obligate intracellular bacterium, grouped within the genusEhrlichiae. Most human cases of ehrlichiosis have been diagnosed in the USA. Two cases have been reported outside of the USA, one in Europe and one in Africa. From 1 January to 30 June 1992, 765 sera from blood donors or other asymptomatic subjects in 8 African countries, including Ivory Coast, Burkina Faso, Mali, Central African Republic, Angola, Zimbabwe, Mozambique and Commores Islands, were tested by indirect immunofluorescence for the presence ofE. chaffeensis antibodies. Positive sera were confirmed by Western immunoblotting. Only two of 765 sera tested were positive. One serum obtained from Burkina Faso had an IgG titer of 1:200 and one from Mozambique had an IgG titer of 1:80. Human ehrlichiosis seems to occur infrequently in Africa, although many more sera from additional African countries need to be evaluated.  相似文献
8.
目的了解安徽省农村地区人群和家畜蜱源立克次体病感染状况和分布特点,为科学防治蜱源立克次体病提供依据。方法 2009年4~5月在安徽省广德县、明光市和怀远县采集农村人群血清613份,家畜血清150份,使用试剂盒,采用间接免疫荧光法(IFA)检测斑点热(SGF)、人单核细胞埃立克体病(HME)和人粒细胞无形体病(HGA)抗体,并进行不同地区、年龄、性别比较。结果 613份人血清中,SGF阳性血清250份,阳性率40.78%;HME阳性血清98份,阳性率15.99%;HGA阳性血清17份,阳性率2.77%。SGF血清抗体存在年龄、性别和地区差异,HME血清抗体存在地区差异;150份家畜血清中,SGF阳性血清91份,阳性率60.67%,HME阳性血清33份,阳性率22%;HGA阳性血清4份,阳性率2.67%;家狗血清HGA阳性率显著高于羊和牛,家畜SGF和HME血清抗体存在地区差异。结论安徽省农村地区存在蜱源立克次体病自然疫源地。  相似文献
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10.
Manifestations of human monocytic ehrlichiosis (HME), a tick-borne infection caused by Ehrlichia chaffeensis, range from asymptomatic disease to fulminant infection and may be particularly severe in persons infected with HIV. We conducted a serologic study to determine the epidemiology of HME in HIV-positive patients residing in an HME-endemic area. We reviewed charts from a cohort of 133 HIV-positive patients who were seen during the 1999 tick season with symptoms compatible with HME (n=36) or who were asymptomatic (n=7). When available, paired plasma samples obtained before and after the tick season were tested by using an indirect immunofluorescence assay (IFA) to detect antibodies reactive to E. chaffeensis. Two symptomatic incident cases were identified by IFA, resulting in a seroincidence of 6.67% among symptomatic HIV-positive participants with paired samples available for testing and 1.64% overall. The baseline seroprevalence of HME was 0%. In contrast to infection in immunocompetent patients, E. chaffeensis infection in HIV-positive persons typically causes symptomatic disease.  相似文献
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