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排序方式: 共有2262条查询结果,搜索用时 0 毫秒
1.
目的 探讨肺炎衣原体(Chlamydia pneumoniae)感染在多发性硬化(MS)发病和进展中的作用和致病机制。方法 选取急性期MS患者31例,缓解期MS患者28例及其他神经系统疾病患者30例,健康对照者30名,应用酶联免疫吸附试验测定患者和对照者血清及脑脊液中肺炎衣原体IgG和IgM抗体水平。结果 急性期MS组、缓解期MS组、其他神经系统疾病组和健康对照组的肺炎衣原体血清IgG分别为48.4%、35.7%、30.0%、23.3%;4组IgM抗体效价分别为12.9%、14.3%、20.0%、10.0%,总体比较差异无统计学意义(P〉0.05);急性期MS组与其他神经系统疾病组的脑脊液IgG和IgM抗体效价分别为0、6.7%和0、0,差异无统计学意义(P〉0.05)。结论 肺炎衣原体的感染或重复感染与MS发病相关不紧密,可能仅为MS的伴随感染。 相似文献
2.
本文用免疫荧光法与 Giemsa 氏法检测明显沙眼患者,两法阳性率相差无几。但在临床肉眼观察认为正常时,其阳性率分别为24.77%及11.58%(P<0.05),差异有显著意义,表明免疫荧光法检测早期沙眼较 Giemsa 氏法敏感。临床认为正常眼,经实验室检查25.56%为阳性,说明开展沙眼的实验室诊断是必要的。 相似文献
3.
目的 探讨在泌尿生殖道有炎症表现的情况下沙眼衣原体 (CT)感染的状况。方法 采用C -C快速法。结果 CT在泌尿生殖道炎症时的阳性率显著高于无炎症表现者 (P <0 .0 5) ;男性在有无炎症表现情况下的CT阳性率差异有显著性 (P <0 .0 5) ,而女性差异无显著性 (P >0 .0 5) ;2 1~ 50岁为高发年龄段。结论 CT感染症状隐匿 ,临床无症状者也应注意CT的检测 ,尤其是女性和高危人群。 相似文献
4.
肺炎衣原体感染小鼠肺组织免疫组化表现 总被引:5,自引:2,他引:3
目的 :通过研究小鼠肺组织免疫组化 ,对肺炎衣原体肺炎的发病机制进行初步的探讨。 方法 :以肺炎衣原体鼻内或静脉接种Icr小鼠 ,在不同时间点处死动物 ,用免疫组化的方法检测小鼠肺炎衣原体肺炎急性期肺组织的病理改变。 结果 :小鼠吸入肺炎衣原体后第 3、7、14天 ,肺组织中肺炎衣原体的免疫过氧化酶染色呈阳性。炎性肺组织阳性染色呈不均一性 ,为局限性分布。肺炎衣原体抗原阳性表达主要在肺泡巨噬细胞、间质细胞以及支气管周围淋巴组织等部位。静脉接种组引起上述类似改变 ,但程度轻 ,肺炎衣原体抗原阳性表达主要集中在肺泡巨噬细胞及间质细胞中。 结论 :免疫组化法检测小鼠肺炎衣原体肺炎急性期肺组织的病理改变 ,有助于肺炎衣原体肺炎急性期的诊断。肺炎衣原体呼吸道局部感染比血行感染的病理改变更为严重 相似文献
5.
微量沙眼衣原体组织培养法的建立及临床应用 总被引:8,自引:0,他引:8
建立了微量沙眼衣原体组织培养法,并应用于不同人群的生殖道标本的沙眼衣原体培养。结果生殖道沙眼衣原体培养的阳性率:男性尿道炎患者147%(9/61);正常孕妇33%(7/210);急性和亚急性盆腔炎女性患者67%(7/104);性病门诊患者182%(49/269)。由于96孔培养板及倒置荧光显微镜的应用,微量培养法试剂用量少、过程简化;自制的抗沙眼衣原体单克隆抗体大大降低了培养后的染色成本,适用于大宗标本。 相似文献
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8.
The occurrence of Chlamydia trachomatis in the semen of men participating in an IVF programme 总被引:1,自引:0,他引:1
Nagy Balint; Corradi Gyula; Vajda Zoltan; Gimes RezsO; Csomor Sandor 《Human reproduction (Oxford, England)》1989,4(1):54-56
Chlamydia trachomatis is one of the most common causative agentsof sexually transmitted diseases. The authors studied the occurrenceof C.trachomatis in the semen of 184 asymptomatic men participatingin the IVF programme. Twenty-six (14.1%) of the 184 tested werepositive for C.trachomatis, these patients and their wives receivingdoxycycline capsules twice, 100 mg on the first day and 100mg/day for the following 13 days. This treatment was effectivein 88.5% of the cases and in the rest, treatment continued witherithromycin 250 mg four times/day for 2 weeks. The authorscompared the semen parameters (cell count, motility, morphology,bovine mucus penetration and hypo-osmotic swelling test) inthe infected and non-infected groups and observed no significantdifference between these two groups 相似文献
9.
A. Schumacher I. Seljeflot A. B. Lerkerød L. Sommervoll J. E. Otterstad H. Arnesen 《Clinical microbiology and infection》2002,8(10):654-661
Objective To investigate if Chlamydia pneumoniae and/or Helicobacter pylori seropositivity is associated with elevated levels of soluble endothelial cell adhesion molecules (sCAMs) as markers of atherosclerotic activity.
Methods Immunoglobulin A (IgA) and IgG antibodies to the two bacteria, soluble intercellular cell adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1 (sVCAM-1) and E-selectin were measured in coronary heart disease (CHD) patients ( n = 193) and age- and sex-matched controls ( n = 193). Two different serological methods were used for the detection of Chlamydia antibodies: Labsystems microimmunofluorescence to detect species-specific C. pneumoniae antibodies and Medac's recombinant enzyme-linked immunosorbent assay to detect genus-specific lipopolysaccharide antibodies.
Results The concentrations of sICAM-1 and E-selectin were higher in CHD patients with positive vs. negative Chlamydia lipopolysaccharide IgA ( P = 0.044 for both). H. pylori antibodies alone did not predict raised levels of sCAMs, but in CHD patients sICAM-1 was increased with IgA seropositivity to both bacteria compared to double seronegativity ( P = 0.034). Concentrations of sVCAM-1 were elevated in CHD patients with double IgA seropositivity compared to those with Chlamydia lipopolysaccharide IgA seropositivity alone ( P = 0.018).
Conclusion Our results may indicate that C. pneumoniae contributes to increased inflammation in CHD, and that this contribution is even more pronounced when present in combination with H. pylori IgA antibodies. 相似文献
Methods Immunoglobulin A (IgA) and IgG antibodies to the two bacteria, soluble intercellular cell adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1 (sVCAM-1) and E-selectin were measured in coronary heart disease (CHD) patients ( n = 193) and age- and sex-matched controls ( n = 193). Two different serological methods were used for the detection of Chlamydia antibodies: Labsystems microimmunofluorescence to detect species-specific C. pneumoniae antibodies and Medac's recombinant enzyme-linked immunosorbent assay to detect genus-specific lipopolysaccharide antibodies.
Results The concentrations of sICAM-1 and E-selectin were higher in CHD patients with positive vs. negative Chlamydia lipopolysaccharide IgA ( P = 0.044 for both). H. pylori antibodies alone did not predict raised levels of sCAMs, but in CHD patients sICAM-1 was increased with IgA seropositivity to both bacteria compared to double seronegativity ( P = 0.034). Concentrations of sVCAM-1 were elevated in CHD patients with double IgA seropositivity compared to those with Chlamydia lipopolysaccharide IgA seropositivity alone ( P = 0.018).
Conclusion Our results may indicate that C. pneumoniae contributes to increased inflammation in CHD, and that this contribution is even more pronounced when present in combination with H. pylori IgA antibodies. 相似文献
10.
A. Spiliopoulou V. Lakiotis A. Vittoraki D. Zavou D. Mauri 《Clinical microbiology and infection》2005,11(9):687-689
Genital Chlamydia trachomatis infection is the leading cause of bacterial sexually transmitted disease in industrialised countries, particularly among young people. The consequences of chlamydial infection may involve urethritis, cervicitis, pelvic inflammatory disease, ectopic pregnancy, tubal factor infertility, epididymitis and prostatitis. In addition, chlamydial infection increases the risk of acquisition of human immunodeficiency virus and has been associated with cervical cancer. Although screening programmes exist in a number of countries, the continuously increasing prevalence of chlamydial infections demonstrates the necessity for health authorities to establish effective screening policies, and the importance of defining a comprehensive European screening policy is emerging. 相似文献