缅甸人类免疫缺陷病毒-1的二重感染

Objective To investigate the occurrence and the characteristics of human immunodeficiency virus (HIV)-1 co/super-infection among high-risk populations in Myanmar.Methods Forty-six HIV-1 positive plasma in Myanmar were collected. Possible cases with HIV-1 co/super infection were identified by discordant sequence results obtained with different polymerase chain reaction (PCR)/sequencing primers or by ambiguous readings in direct sequencing. HIV-1 quasispecies in plasma were then characterized by clonal sequence analysis of independent PCR-clones generated by TA cloning method. Thereafter, their phylogeny and recombinant structure were investigated. Results Co/super infection was identified in 3 (6.5 %) cases among the 46 screened HIV-1 positive patients.All of these three patients were heterosexuals and were co/super infected with CRF01_AE/subtype B′recombinants. Conclusions HIV-1 co/super infections are relatively common and provide a prerequisite for rapid generation of new recombinant forms in Myanmar.     

昆明地区吸毒人群和异性性传播HIV-1的分子流行病学

目的探讨昆明地区吸毒人群和异性性传播感染者HIV-1的分子流行病学特点。方法收集1994~2002年26例昆明地区吸毒人群和异性性传播HIV-1阳性者的血浆,采用BigDye链终止反应试剂盒测定2.6-kb gag-RT区的核苷酸序列,确定HIV-1的基因型分布。结果CRF07_BC和CRF08_BC是吸毒人群中HIV-1的主要基因型;1例吸毒者感染了亚型B′。同样,大多数异性性传播HIV-1感染者的基因型也以CRF07_BC,CRF08_BC和亚型B'为主。结论昆明地区异性性传播HIV-1感染者中流行的病毒基因型来自当地吸毒人群。     

缅甸人类免疫缺陷病毒-1的二重感染

目的 了解缅甸高危人群HIV-1二重感染的发生情况与特征.方法 收集缅甸HIV-1阳性血浆46份,通过不同PCR测序引物得到不同测序结果或直接测序时碱基模糊,从而确定可能为二重感染病例,然后采用TA克隆的方法对单个PCR克隆进行测序,找到血浆标本中的HIV-1准种,并分析其系统发生和重组结构.结果 46例HIV-1阳性患者中,3例为HIV-1二重感染,发生率为6.5%.此3例患者均通过异性性接触感染,均为CRF01_AE和B′以及两者的重组病毒准种构成的混合感染.结论 缅甸高危人群HIV-1的二重感染较为常见,为快速产生新的重组病毒株提供了基础.

Abstract：

Objective To investigate the occurrence and the characteristics of human immunodeficiency virus (HIV)-1 co/super-infection among high-risk populations in Myanmar.Methods Forty-six HIV-1 positive plasma in Myanmar were collected. Possible cases with HIV-1 co/super infection were identified by discordant sequence results obtained with different polymerase chain reaction (PCR)/sequencing primers or by ambiguous readings in direct sequencing. HIV-1 quasispecies in plasma were then characterized by clonal sequence analysis of independent PCR-clones generated by TA cloning method. Thereafter, their phylogeny and recombinant structure were investigated. Results Co/super infection was identified in 3 (6.5 %) cases among the 46 screened HIV-1 positive patients.All of these three patients were heterosexuals and were co/super infected with CRF01_AE/subtype B′recombinants. Conclusions HIV-1 co/super infections are relatively common and provide a prerequisite for rapid generation of new recombinant forms in Myanmar.     

云南省昆明地区高危人群HIV-1二重感染的研究

目的了解云南省昆明地区高危人群艾滋病病毒I型（HIV-1）二重感染的发生情况与特征。方法筛选昆明地区高危人群出现HIV-1二重感染的病例。通过不同的PCR／测序引物得到不同的测序结果,或直接测序时碱基模糊不清而确定的可疑二重感染病例,然后采用TA克隆的方法对单个PCR克隆进行测序,找到血浆标本中的HIV-1准种,并分析其系统发生和重组结构。结果昆明地区的高危人群中,HIV-1二重感染的发生率为7．7％（2／26）。2名具有高危异性性接触史的HIV-1感染者,分别为CRF01_AE和CRF08-BC或CRF07-BC的二重感染。结论昆明地区高危人群HIV-1的二重感染较为常见,这为快速产生新的重组病毒株提供了基础。该研究提示,该地区高危人群经常暴露于新的病毒株,将加速基因型内重组的发生。     

缅甸人类免疫缺陷病毒-1的二重感染

Objective To investigate the occurrence and the characteristics of human immunodeficiency virus (HIV)-1 co/super-infection among high-risk populations in Myanmar.Methods Forty-six HIV-1 positive plasma in Myanmar were collected. Possible cases with HIV-1 co/super infection were identified by discordant sequence results obtained with different polymerase chain reaction (PCR)/sequencing primers or by ambiguous readings in direct sequencing. HIV-1 quasispecies in plasma were then characterized by clonal sequence analysis of independent PCR-clones generated by TA cloning method. Thereafter, their phylogeny and recombinant structure were investigated. Results Co/super infection was identified in 3 (6.5 %) cases among the 46 screened HIV-1 positive patients.All of these three patients were heterosexuals and were co/super infected with CRF01_AE/subtype B′recombinants. Conclusions HIV-1 co/super infections are relatively common and provide a prerequisite for rapid generation of new recombinant forms in Myanmar.