冠心病患病与脉压关系的调查

冠心病 (CHD)最常见的重要原因之一是高血压引起的临床征候群。近来的前瞻性研究表明 ,收缩压 (SBP)升高比舒张压 (DBP)的改变更能显示CHD发病的危险性 ;也提出脉压 (PP)是CHD的独立危险因子。本文对一组中老年人群进行了调查 ,以探讨PP作为危险因素在CHD的患病危险。1.对象与方法 :2 0 0 1年 5～ 7月对驻北京部队干部 2 4 6 2名中老年人群进行了调查 ,年龄 4 0～ 91岁 ,平均 (6 2 .34±10 .1)岁 ,其中 4 0～ 5 9岁 937例 ,6 0～ 79岁 14 12例 ,≥ 80岁77例 ,男性 2 15 7例 ,占 87.6 1% ,女性 30 5例 ,占 12 .39%。CHD的诊断是根…     

Targeting fibroblast activation protein inhibits endothelial-mesenchymal transition by affecting cancer-associated fibroblasts derived exosomes北大核心CSCD

Aim To investigate whether targeted inhibition of fibroblast activation protein (FAP) can inhibit the endothelial-to-mesenchymal transition (EndMT) of vascular endothelial cells by affecting exosomes (Exo) of cancer-associated fibroblasts (CAFs) and explore the underlying mechanisms. Methods Primary CAFs and peri-tumor fibroblasts (PTFs) were obtained from lung cancer and peri-cancer tissues, and CAFs-exo and PTFs-exo were collected from culture medium, respectively. Exosomes from CAFs treated with specific FAP inhibitor (3.3 nmol • L-1 SP13786) for 24 h were named as Anti-FAP-exo. HMEC-1 cells were incubated in equal volumes of RPMI 1640, PTFs-exo, CAFs-exo and anti-FAP-exo respectively and named as control group, PTF group, C AF group and anti-FAP group. The scratch assay, Transwell invasion assay and angiogenesis assay were used to detect the migration ability, invasion ability and angiogenesis ability of HMEC-1 cells. Immunofluorescence, immunohistochemistry and Western blot were used to detect EndMT-associated protein expression. Results The migration ability, invasion ability and angiogenesis ability of HMEC-1 cells of CAF group were significantly higher than those of PTF group, whereas there was no significant difference between that of anti-FAP group and PTF group. HMEC-1 cells of CAF group had higher expression of α-SMA, SM22α, p-Stat3 and Snail, and lower expression of CD31 and VE-cadherin than that of PTF group. In addition, HMEC-1 cells of Anti-FAP group had lower expression of α-SMA, SM22α, pStat3 and Snail, and higher expression of CD31 and VE-cadherin than that of CAF group. Conclusions Specific inhibition of FAP could indirectly inhibit the migration ability, invasion ability and angiogenesis ability of vascular endothelial cells via affecting CAFs-exo and Stat3-snail-EndMT pathway may be the potential mechanism. © 2023 Publication Centre of Anhui Medical University. All rights reserved.