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1.
參附注射液對敗血性休克大鼠心肌損傷的影響   总被引:12,自引:1,他引:11  
目的:觀察參附注射液對敗血性休克大鼠心肌損傷的保護作用并探討其可能機制。方法:Wistar大鼠隨機分為假手術組(C)、休克組(S)、參附治療組(SF),各組均為 16 只。采用改良的盲腸結扎穿孔(CLP)方法復制敗血性休克模型,觀察術后6 h和18 h的心肌組織病理改變;免疫組織化學技術檢測心肌組織中核轉錄因子 κB(NF κB)、細胞間粘附分子 1(ICAM 1)的表達和分布;用ELISA法檢測血漿中腫瘤壞死因子 α(TNF α)、白細胞介素 10(IL 10)的含量,連續監測動脈壓及心率變化。結果:較之C組, 6 h時S組大鼠動脈壓即明顯下降,心率增快(P<0.01),到18 h心率、動脈壓均極度降低(P<0.01),而SF組18 h才出現 MAP下降(P<0.05)。與同時點 S組比較,SF組心肌組織病理損害明顯減輕;SF組CLP后心肌組織 NF κB及 ICAM 1 的陽性表達比 S組明顯降低(P<0.01);SF組血漿中TNF α含量較S組明顯降低(P<0.01),18 h時 IL 10 含量較 S組明顯降低(P<0.01)。結論:參附注射液能抑制敗血性休克后心肌組織NF κB的活化及ICAM 1、TNF α、IL 10的表達從而起到心肌保護的作用。  相似文献
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Objective To study the effects of vitamin E on the proliferation and collagen synthesis of rat hepatic stellate cells treated with interleukin-2 (IL-2 ) or tumor necrosis factor-α (TNF-α). Methods Hepatic stellate cells were isolated from male Sprague-Dawley rats by using modified Friedman’s method. Using the isolated cells cultured and treated with IL-2 or TNF-α, we studied the effects of vitamin E on their proliferation and collagen synthesis through an (3)H-thymidine and (3)H-proline incorporation assay, as well as through observation of these cells under a contrary phase microscope. Results Adding IL-2 increased the both proliferation and collagen synthesis of hepatic stellate cells. Their proliferation was also increased by the addition of TNF-α, although it decreased collagen synthesis. Vitamin E had marked inhibitory effects on the ability of cells treated with IL-2 or TNF-α to reproduce or synthesize collagen. Conclusion Vitamin E can inhibit the proliferation and collagen synthesis of hepatic stellate cells. It is possible that vitamin E affects liver fibrosis through these activities.  相似文献
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Background Many cytokines have been found to increase the insulin resistance during pregnancy complicated by glucose metabolism disorder. This study aimed to investigate which comes first, the changes of some cytokines or the abnormal glucose metabolism.
Methods This nested case-control study was undertaken from January 2004 to March 2005. Twenty-two women with gestational diabetes mellitus (GDM), 10 with gestational impaired glucose tolerance (GIGT), and 20 healthy pregnant women were chosen from the women who had visited the antenatal clinics and had blood samples prospectively taken and kept during their visit. The levels of tumor necrosis factor-α (TNF-α), leptin and adiponectin were determined. One-way ANOVA analysis and bivariate correlation analysis were used to assess the laboratory results and their relationship with body mass index (BMI). Results Women with GDM have the highest values of TNF-α and leptin and the lowest value of adiponectin compared with those with GIGT and the healthy controls (P 〈0.01) at 14-20 weeks of gestation. This was also found when these women progressed to 24-32 weeks. The significantly increased levels of TNF-α and leptin and the decreased level of adiponectin were found at the different periods of gestation within the same group. Positive correlation was shown between the levels of TNF-α and leptin at the two periods of gestation with the BMI at 14-20 weeks, while adiponectin was negatively correlated (P 〈0.05).
Conclusions The concentrations of TNF-α, leptin and adiponectin may change before the appearance of the abnormal glucose level during pregnancy. Further studies are required to verify the mechanism of this alteration and whether the three cytokines can be predictors for GDM at an early staqe of preqnancy.  相似文献
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丁咯地尔与前列腺素E1联合治疗急性脑梗死的临床价值   总被引:7,自引:2,他引:5  
目的探讨丁咯地尔与前列腺素E1联合治疗急性脑梗死的临床价值。方法88例急性脑梗死患者分为观察组和对照组,对照组给予右旋糖苷加川芎嗪静脉滴注,观察组在对照组治疗基础上加用盐酸丁咯地尔氯化钠注射液和前列腺素E1静脉滴注,均治疗14d。随访3个月,检测血清高敏C反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)水平,作临床神经功能缺损程度评分判定疗效。结果观察组和对照组治疗后血清hs-CRP、TNF-α含量均较治疗前显著降低(P<0.05),观察组血清hs-CRP、TNF-α降低程度明显高于对照组(P<0.05)。观察组的显效率和总有效率均明显高于对照组(P<0.05)。结论丁咯地尔与前列腺素E1联合对控制急性脑梗死具有良好疗效。  相似文献
6.
人工晶体植入术后炎性并发症与TNF-α和IL-8的关系   总被引:6,自引:5,他引:1  
目的 :探讨血浆和房水肿瘤坏死因子 - α( TNF- α)、白细胞介素 - 8( IL- 8)与人工晶状体植入术后炎性并发症的关系。方法 :采用放射免疫法对 40例晶状体囊外摘除后房型人工晶状体植入术后 3种不同级别炎性并发症家兔进行血浆和房水 TNF-α和 IL- 8测定。结果 :40只家兔在血浆和房水内 TNF-α和 IL- 8术后较术前均升高 ,且与术后炎性并发症呈正相关。结论 :血浆和房水 TNF-α和 IL- 8水平在晶状体囊外摘除后房型人工晶状体植入后升高 ,且升高水平与炎性并发症呈正相关 ,TNF- α和 IL- 8可作为监测术后恢复情况、估计预后及转归的实验室指标  相似文献
7.
目的:探讨参附注射液(SF)对肾缺血再灌注损伤大鼠P38丝裂原活化蛋白激酶(MAPK)、肿瘤坏死因子(TNF-α)表达的影响。方法:采用切除右肾,无创动脉夹夹闭左肾肾蒂45 min,再灌注2 h制作在体肾缺血再灌注损伤模型。动物随机分为3组:预处理组、缺血再灌注组和对照组。免疫组织化学检测肾组织P38 MAPK蛋白含量,酶联免疫吸附实验(ELISA)检测肾组织TNF-α的表达。结果:预处理组与缺血再灌注组相比,肾脏病理损伤明显减轻,P38 MAPK、TNF-α蛋白含量显著降低(均为P<0.05)。结论:SF可能通过抑制P38 MAPK的表达,从而下调TNF-α的表达,发挥其肾脏保护作用。  相似文献
8.
枸杞多糖对2型糖尿病大鼠血糖、血脂及TNF-α水平的影响   总被引:6,自引:0,他引:6  
目的观察枸杞多糖对2型糖尿病大鼠血糖、胰岛素、血脂及血清TNF-α水平的影响。方法采用高脂高糖饲料喂养8周联合腹腔注射小剂量链脲佐菌素的方法,建立2型糖尿病大鼠模型。将未予上述处理的大鼠设为正常对照组,成模大鼠按血糖再随机分为糖尿病对照组、枸杞多糖(LBP)低、中、高剂量干预组和二甲双胍组,药物干预6周后,处死动物取血测定血脂、空腹血糖,应用放射免疫分析法测定空腹胰岛素及血清TNF-α水平。结果LBP中、高剂量干预组及二甲双胍组空腹血糖水平较糖尿病对照组降低(P〈0.05,P〈0.01),较正常对照组高(P〈0.05);LBP高剂量干预组及二甲双胍组ISI较糖尿病对照组升高(P〈0.05);LBP中、高剂量干预组血清TC水平较糖尿病对照组降低(P〈0.05),LBP各剂量组血清TNF-α水平较糖尿病对照组有所下降,但差异无统计学意义(P〉0.05)。二甲双胍组血清TNF-α水平较糖尿病对照组降低(P〈0.05)。结论LBP能够降低2型糖尿病大鼠血糖及血脂水平,增加组织的胰岛素敏感性,改善糖尿病大鼠糖脂代谢紊乱及胰岛素抵抗。  相似文献
9.
Background Leptin is a protein mainly secreted by adipocytes, and the major function of leptin was its role in body weight regulation. It is suggested that increased levels of circulating leptin may contribute to anorexia in pathologic conditions including chronic obstructive pulmonary disease (COPD). Recent studies have provided evidence for a link between leptin and proinflammatory cytokines such as tumor necrosis factor-α (TNF-α). This study aimed to explore the role of serum leptin in the malnutrition of COPD patients, and to observe the changes of serum leptin levels during acute exacerbation, also to investigate relationship between leptin and TNF-α. Methods Seventy-two COPD patients and 34 control subjects participated in this study. Seventy-two COPD patients were divided into 3 groups: group COPD IA (patients without malnutrition during acute exacerbation, n=25), group COPD IB (patients without malnutrition during stable disease, n=29), group COPD II (patients with malnutrition during stable disease, n=18). To eliminate the effect of sex differences, all patients and controls were male. Body mass index (BMI), percent ideal body weight (IBW%), triceps skin-fold thickness (TSF), mid-upper arm circumference (MAC), mid-upper arm muscle circumference (MAMC), serum leptin and TNF-α levels, serum prealbumin (PA), serum transferrin (TF), serum albumin (Alb), total lymphocytes count (TLC), forced expiratory volume in one second (FEV(1)), maximal inspiration pressure (MIP) and maximal expiration pressure (MEP) were measured in all participants. Leptin levels were measured by radioimmunoassay. TNF-α levels were measured by ELISA. The between group difference and correlation of these parameters were analyzed. Results Serum leptin levels were significantly lower in group COPD II [(4.07±3.42) ng/ml] than in group COPD IB [(9.72±6.67) ng/ml] and controls [(8.21±5.41) ng/ml] (P&lt;0.05). There was no statistically significant difference in serum leptin levels between group COPD IA [(10.82±6.40) ng/ml], group COPD IB [(9.72±6.67) ng/ml] and controls [(8.21±5.41) ng/ml]. There was no statistically significant difference in serum TNF-α levels between group COPD II [(8.03±3.37) pg/ml], group COPD IA [(8.90±1.60) pg/ml], and group COPD IB [(7.25±2.08) pg/ml]. There was no significant correlation between leptin and TNF-α in any group. Conclusions Leptin was not involved in anorexia and weight loss of COPD patients. There was no statistically significant difference in serum leptin levels between COPD patients during stable stage and acute exacerbation, and there was no significant correlation between TNF-α and leptin during the regulation of the energy balance in COPD patients.  相似文献
10.
目的 :探讨急性脑梗塞患者血清中白细胞介素 6((IL 6)及肿瘤坏死因子 α(TNF α)在发病中的作用及临床意义。方法 :采用酶联免疫法测定 45例急性脑梗塞患者血清IL 6和TNF α含量 ,并与 3 6例正常对照组比较。结果 :急性脑梗塞患者血清IL 6和TNF α水平均显著高于正常组 ,恢复期两种细胞因子有下降的趋势 ,但仍高于正常。结论 :脑梗塞发病过程中存在IL 6和TNF α介导的炎症和免疫反应 ,IL 6和TNF α的测定对急性脑梗塞的治疗和预后判断有一定价值  相似文献
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