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1.
Huang H  Chen L  Wang H  Xiu B  Li B  Wang R  Zhang J  Zhang F  Gu Z  Li Y  Song Y  Hao W  Pang S  Sun J 《中华医学杂志(英文版)》2003,116(10):1488-1491
Objective To evaluate the restoration of function after spinal cord injury (SCI) in patients of different ages who have underwent intraspinal transplantation of olfactory ensheathing cells (OECs). Methods One hundred and seventy-one SCI patients were included in this study. Of them, 139 were male and 32 were female, with age ranging from 2 to 64 years (mean, 34.9 years). In all SCI patients the lesions were injected at the time of operation with OECs. According to their ages, the patients were divided into 5 groups: ≤20 years group (n=9), 21-30 years group (n=54), 31-40 years group (n=60), 41-50 years group (n=34) and>51 years group (n=14). The spinal cord function was assessed based on the American Spinal Injury Association (ASIA) Classification System before and 2-8 weeks after OECs transplantation. One-way ANOVA and q test were used for statistical analysis, and the data were expressed as mean±SD. Results After surgery, the motor scores increased by 5.2±4.8, 8.6±8.0, 8.3±8.8, 5.7±7.3 and 8.2±7.6 in 5 age groups respectively (F=1.009, P=0.404); light touch scores increased by 13.9±8.1, 15.5±14.3, 12.0±14.4, 14.1±18.5 and 24.8±25.3 respectively (F=1.837, P=0.124); and pin prick scores increased by 11.1±7.9, 17.2±14.3, 13.2±11.8, 13.6±13.9 and 25.4±24.3 respectively (F=2.651, P=0.035). Restoration of pin prick in >51 years group was better than other age groups except 21-30 years group. Conclusion OECs transplantation can improve the neurological function of spinal cord of SCI patients regardless of their ages. Further research into the long-term outcomes of the treatment will be required.  相似文献
2.
建立人工膀胱反射弧恢复脊髓损伤患者排尿功能的初步报告   总被引:30,自引:0,他引:30  
目的:探索建立人工膀胱反射弧恢复脊髓损伤患者排尿功能的治疗途径。方法:对3例圆锥上脊髓损伤(SCI)患者行两侧L5-S2或S1-S3前根吻合,经10-12个月轴索再生后,检测膀胱排尿功能。结果:建立人工膀胱反射弧手术后,通过刺激躯体传入神经,可经内脏传出神经引发膀胱内压升高,在膀胱充盈较满时能引起排尿反应。结论:建立人工膀胱反射弧对SCI患者排尿有一定作用,但尚需进一步研究完善。  相似文献
3.
建立人工膀胱反射弧的实验研究   总被引:29,自引:2,他引:27  
目的:建立犬人工膀胱反射通路,以恢复脊髓损伤后的膀胱功能。方法:将右L5前根近端与右S2前根远端在硬膜囊内吻合,经轴突再生后,建立“膝腱-脊髓中枢-膀胱”这一新的人工膀胱反射通路,通过刺激膝腱激发排尿。结果:T10平面截瘫前后电刺激右L5后根,均可在吻合口远端记录到动作电位,其形态和波幅与对照组相似;截瘫后48h,通过电刺激传入神经,经新建的反射弧引出的膀胱收缩平均可达正常的65%,敲击膝腱引出的  相似文献
4.
大鼠脊髓压迫性损伤后神经细胞的死亡方式   总被引:18,自引:2,他引:16  
目的:观察实验性脊髓损伤后神经细胞死亡方式。方法:应用凋亡细胞原位末端标记法,HE染色,对脊髓组织进行定量形态学分析,结果;在脊髓损伤区及相邻区域中发现少量神经元以大量胶质细胞凋亡,结论:脊髓损伤后的神经细胞的凋亡是继发损伤期的重要病理变化。  相似文献
5.
复方丹参对大鼠实验性脊髓损伤的保护作用   总被引:17,自引:0,他引:17  
目的 :探讨复方丹参对脊髓继发性损伤的保护作用 ,并与甲基强的松龙 (MP)进行对照。方法 :制备鼠脊髓腰膨大半切模型 ,随机分为单纯损伤组、损伤加复方丹参治疗组、损伤加 MP治疗组。测定用药后不同时间脊髓组织线粒体 SOD活性和 MDA浓度以及血液流变学改变 ,同时进行运动诱发电位 (MEP)检测 ;斜板实验评估用药后 2 ,4周复方丹参对运动功能的影响。结果 :复方丹参处理后脊髓组织 MDA浓度明显低于各时相点对照组 ,SOD活性显著升高 (P<0 .0 1) ,与 MP治疗组无明显差异 (P>0 .0 5 ) ;损伤节段血流量及 MEP、斜板实验也有所改善。结论 :复方丹参可以缓解脊髓损伤后的脂质过氧化反应 ,改善微循环 ,从而发挥脊髓保护作用  相似文献
6.
人脐血干细胞移植促进大鼠脊髓损伤神经恢复   总被引:16,自引:0,他引:16  
目的观察移植人脐血CD34 干细胞是否可以存活、分化、促进脊髓损伤的行为和功能恢复.方法26只Wister大鼠随机分成移植组和对照组.移植组行脊髓半切术并移植5-溴脱氧尿核苷(Brdu)标记的脐血CD34 细胞,对照组行脊髓半切术后注射PBS液.采用改良Tarlov评分标准对移植组和对照组所有大鼠在术前和术后24 h、1、2、3、4周进行运动功能评价.组织学和免疫组化分析移植细胞的定位和分化情况.结果移植后在脊髓病理切片中出现Brdu标记人脐血细胞,激光扫描共聚焦显微镜通过免疫荧光双标检测到7%Brdu阳性细胞表达神经胶质元纤维酸性蛋白(GFAP),2%Brdu阳性细胞表达神经元核抗原(NeuN).神经功能检测发现1周后移植组功能恢复较对照组具有显著性差异(P<0.05).结论移植人脐血干细胞可以促进脊髓损伤的行为和功能恢复,为神经损伤治疗提供了有用的细胞源.  相似文献
7.
Background The treatment of spinal cord injury is still a challenge. This study aimed at evaluating the therapeutical effectiveness of neurons derived form mesenchymal stem cells (MSCs) for spinal cord injury.Methods In this study, rhesus MSCs were isolated and induced by cryptotanshinone in vitro and then a process of RT-PCR was used to detect the expression of glutamic acid decarboxylase (GAD) gene. The induced MSCs were tagged with Hoechst 33342 and injected into the injury site of rhesus spinal cord made by the modified Allen method. Following that, behavior analysis was made after 1 week, 1 month, 2 months and 3 months. After 3 months, true blue chloride retrograde tracing study was also used to evaluate the re-establishment of axons pathway and the hematoxylin-eosin (HE) staining and immunohistochemistry were performed after the animals had been killed.Results In this study, the expression of mRNA of GAD gene could be found in the induced MSCs but not in primitive MSCs and immunohistochemistry could also confirm that rhesus MSCs could be induced and differentiated into neurons. Behavior analysis showed that the experimental animals restored the function of spinal cord up to grade 2-3 of Tarlov classification. Retrograde tracing study showed that true blue chollide could be found in the rostral thoracic spinal cords, red nucleus and sensory-motor cortex.Conclusions These results suggest that the transplantation is safe and effective.  相似文献
8.
三七总皂甙对脊髓损伤后脊髓血流量和感觉诱发电位的影响   总被引:16,自引:0,他引:16  
目的 探讨脊髓损伤后三七总皂甙 (totalsaponinsofpanaxnotoginseseng ,PNS)对脊髓血流量及神经功能的影响。方法 Wistar大鼠 2 0只 ,随机分为生理盐水对照组 (NS组 )和PNS治疗组 (PNS组 )。Allen’s打击法 5 0gcm ( 5g× 10cm)致伤大鼠L1 L2 段脊髓 ,立即腹腔注射PNS(生理盐水配制 ,10ml kg ,2 0mmol L) ,于伤前、伤后即刻、1、3、6、2 4h分别记录T13段脊髓血流量、脊髓感觉诱发电位。结果 与盐水对照组比较 ,应用PNS后 ,大鼠SCBF、SEP明显优于生理盐水对照组 (P <0 0 5 )。结论 大剂量PNS能有效改善损伤早期脊髓微循环 ,保护脊髓神经功能  相似文献
9.
Background Axonal regeneration in lesioned mammalian central nervous system is abortive, and this causes permanent disabilities in individuals with spinal cord injuries. This paper studied the action of neural stem cell (NSC) in promoting corticospinal axons regeneration and synapse reformation in rats with injured spinal cord. Methods NSCs were isolated from the cortical tissue of spontaneous aborted human fetuses in accordance with the ethical request. The cells were discarded from the NSC culture to acquire NSC-conditioned medium. Sixty adult Wistar rats were randomly divided into four groups (n=15 in each): NSC graft, NSC medium, graft control and medium control groups. Microsurgical transection of the spinal cord was performed in all the rats at the T11. The NSC graft group received stereotaxic injections of NSCs suspension into both the spinal cord stumps immediately after transection; graft control group received DMEM injection. In NSC medium group, NSC-conditioned medium was administered into the spinal cord every week; NSC culture medium was administered to the medium control group. Hindlimb motor function was assessed using the BBB Locomotor Rating Scale. Regeneration of biotin dextran amine (BDA) labeled corticospinal tract was assessed. Differentiation of NSCs and the expression of synaptophysin at the distal end of the injured spinal cord were observed under a confocal microscope. Group comparisons of behavioral data were analyzed with ANOVA. Results NSCs transplantation resulted in extensive growth of corticospinal axons and locomotor recovery in adult rats after complete spinal cord transection, the mean BBB scores reached 12.5 in NSC graft group and 2.5 in graft control group (P〈 0.05). There was also significant difference in BBB score between the NSC medium (11.7) and medium control groups (3.7, P〈 0.05). BDA traces regenerated fibers sprouted across the lesion site and entered the caudal part of the spinal cord. Synaptophysin expression colocalized with BDA positive axons and neurons distal to the injury site. Transplanted cells were found to migrate into the lesion, but not scatter along the route of axon grows. The cells differentiated into astrocytes or oligodendrocytes, but not into the neurons after transplantation. Furthermore, NSC medium administration did not limit the degree of axon sprouting and functional recovery of the injured rats compared to the NSC graft group. Conclusions Human embryonic neural stem cells can promote functional corticospinal axons regeneration and synapse reformation in the injured spinal cord of rats. The action is mainly through the nutritional effect of the stem cells on the spinal cord.  相似文献
10.
无脊髓损伤颈椎骨折脱位的外科治疗   总被引:14,自引:0,他引:14  
目的:探讨无脊髓损伤的颈椎骨折脱位的手术治疗方法和技术改进。方法:对22例无脊髓损伤的颈椎骨折或骨折脱位,其中新鲜损伤8例,陈旧性损伤14例,采用经前路伤椎椎体次全切除减压,复位、自体髂骨植骨及AO纯钛带锁钢板固定的方法进行手术治疗。结果:术后全部病例经随访观察,症状获得改善,7例完全恢复。植骨在3个月内牢固融合,术后6个月,16例重返工作,3例残留手指麻木。颈椎椎间高度、生理曲度维持良好,无钢板  相似文献
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