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排序方式: 共有619条查询结果,搜索用时 15 毫秒
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目的 探讨负荷渐增式训练对老年小鼠骨骼肌卫星细胞腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)磷酸化的影响。方法 实验小鼠分为 3 组:青年对照组(YC组,n=12)、老年对照组(OC组,n=12)与老年运动组(OT组,n=12)。OT组进行负荷渐增式训练,流式细胞分选技术分离CD45-/CD31-/Sca1-/VCAM(CD106)+细胞群体,分选细胞通过desmin、Myod肌原性染色以及成肌分化诱导培养进行肌卫星细胞鉴定,免疫组化结合Western blotting方法检测肌卫星细胞p-AMPK水平。结果 YC组骨骼肌卫星细胞AMPK及p-AMPK表达水平显著高于OC组(P<0.05);OT组与OC组AMPK表达无明显变化(P>0.05),而OT组p-AMPK表达水平显著高于OC组(P<0.05)。结论 负荷渐增式训练可促进老年小鼠骨骼肌卫星细胞AMPK磷酸化,改善老年小鼠骨骼肌能量代谢。 相似文献
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BMP2诱导骨骼肌成骨的研究进展 总被引:4,自引:1,他引:4
20世纪90年代以来,组织工程技术不断发展,在组织工程化骨领域取得了较大进展,然而,迄今为止,组织工程化骨修复缺损,离临床的要求还有较大距离。目前认为,主要问题是种子细胞在一定传代后发生衰老而不能继续增殖。骨组织工程种子细胞多集中在骨髓基质干细胞的研究上,实际上机体骨髓中所含的基质干细胞数量非常有限^[1], 相似文献
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Michael R. Davies Steven Garcia Stanley Tamaki Xuhui Liu Solomon Lee Anthony Jose Jason H. Pomerantz Brian T. Feeley 《Journal of orthopaedic research》2018,36(5):1370-1376
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E. Martha Pérez Armendariz Monica Norcini Beatriz Hernández-Tellez Andrés Castell-Rodríguez Cristina Coronel-Cruz Raquel Guerrero Alquicira Alexandra Sideris Esperanza Recio-Pinto 《Acta histochemica》2018,120(3):168-178
Previous studies have shown that following peripheral nerve injury there was a downregulation of the gap junction protein connexin 36 (Cx36) in the spinal cord; however, it is not known whether Cx36 protein is expressed in the dorsal root ganglia (DRGs), nor if its levels are altered following peripheral nerve injuries. Here we address these aspects in the adult rat lumbar DRG. Cx36 mRNA was detected using qRT-PCR, and Cx36 protein was identified in DRG sections using immunohistochemistry (IHC) and immunofluorescence (IF). Double staining revealed that Cx36 co-localizes with both anti-β-III tubulin, a neuronal marker, and anti-glutamine synthetase, a satellite glial cell (SGC) marker. In neurons, Cx36 staining was mostly uniform in somata and fibers of all sizes and its intensity increased at the cell membranes. This labeling pattern was in contrast with Cx36 IF dots mainly found at junctional membranes in islet beta cells used as a control tissue. Co-staining with anti-Cx43 and anti-Cx36 showed that whereas mostly uniform staining of Cx36 was found throughout neurons and SGCs, Cx43 IF puncta were localized to SGCs. Cx36 mRNA was expressed in normal lumbar DRG, and it was significantly down-regulated in L4 DRG of rats that underwent sciatic nerve injury resulting in persistent hypersensitivity. Collectively, these findings demonstrated that neurons and SGCs express Cx36 protein in normal DRG, and suggested that perturbation of Cx36 levels may contribute to chronic neuropathic pain resulting from a peripheral nerve injury. 相似文献
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Satellite and Mobile Wireless Transmission of Focused Assessment with Sonography in Trauma 总被引:1,自引:0,他引:1
Christofer A. Strode MD Bernard J. Rubal PhD Robert T. Gerhardt MD MPH Frank L. Christopher MD James R. Bulgrin BS-EE E. Sterling Kinkler Jr. PE MS Terry D. Bauch MD Sheri Y. N. Boyd MD 《Academic emergency medicine》2003,10(12):1411-1414
Objectives: Focused assessment with sonography in trauma (FAST) can define life‐threatening injuries in austere settings with remote real‐time review by experienced physicians. This study evaluates vest‐mounted microwave, satellite, and LifeLink communications technology for image clarity and diagnostic accuracy during remote transmission of FAST examinations. Methods: Using a SonoSite, FAST was obtained on three patients with pericardial and intraperitoneal effusions and two control subjects in a remotely located U.S. Army Combat Support Hospital. A miniature vest‐mounted video transmitter attached to the SonoSite sent wireless ultrasound video 20 m to a receiving antenna. The signal was then transferred over VSAT satellite systems at 512 kilobaud per second (kbps), INMARSAT satellite systems at 64 kbps, and over LifeLink on a moving ambulance through a metropolitan wireless traffic–management network. Clarity and absence or presence of effusions were recorded by 15 staff emergency physicians. Results: Average sensitivity, specificity, and accuracy were 87% (95% confidence interval [CI] = 79% to 95%), 85% (95% CI = 81% to 89%), and 86% (95% CI = 82% to 90%) for the Premier Wireless Vest; 98% (95% CI = 97% to 99%), 83% (95% CI = 75% to 91%), and 86% (95% CI = 82% to 90%) for VSAT; 95% (95% CI = 94% to 96%), 70% (95% CI = 58% to 82%), and 75% (95% CI = 70% to 80%) for INMARSAT; and 82% (95% CI = 73% to 91%), 83% (95% CI = 74% to 92%), and 82% (95% CI = 78% to 86%) for LifeLink with clarity of 3.0 (95% CI = 2.7 to 3.3), 2.9 (95% CI = 2.6 to 3.2), 1.3 (95% CI = 1.2 to 1.4), and 2.1 (95% CI = 1.8 to 2.4), respectively. Conclusions: Accuracy correlated with clarity. Roaming vest transmission of FAST provides interpretable, diagnostic imagery at the distances used in this study. VSAT provided the best clarity and diagnostic value with the lighter, more portable INMARSAT serving a lesser role for remote clinical interpretation. LifeLink performed well, and further infrastructure improvements may increase clarity and accuracy. 相似文献
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Elisa Negroni Teresa Gidaro Anne Bigot Gillian S. Butler‐Browne Vincent Mouly Capucine Trollet 《Neuropathology and applied neurobiology》2015,41(3):270-287
Despite considerable progress to increase our understanding of muscle genetics, pathophysiology, molecular and cellular partners involved in muscular dystrophies and muscle ageing, there is still a crucial need for effective treatments to counteract muscle degeneration and muscle wasting in such conditions. This review focuses on cell‐based therapy for muscle diseases. We give an overview of the different parameters that have to be taken into account in such a therapeutic strategy, including the influence of muscle ageing, cell proliferation and migration capacities, as well as the translation of preclinical results in rodent into human clinical approaches. We describe recent advances in different types of human myogenic stem cells, with a particular emphasis on myoblasts but also on other candidate cells described so far [CD133+ cells, aldehyde dehydrogenase‐positive cells (ALDH+), muscle‐derived stem cells (MuStem), embryonic stem cells (ES) and induced pluripotent stem cells (iPS)]. Finally, we provide an update of ongoing clinical trials using cell therapy strategies. 相似文献
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Heat‐Stress effects on the myosin heavy chain phenotype of rat soleus fibers during the early stages of regeneration 下载免费PDF全文
Yasuharu Oishi PhD Roland R. Roy PhD Tomonori Ogata PhD Yoshinobu Ohira PhD 《Muscle & nerve》2015,52(6):1047-1056
Introduction: We investigated heat‐stress effects on the adult myosin heavy chain (MyHC) profile of soleus muscle fibers at an early stage of regeneration. Methods: Regenerating fibers in adult rats were analyzed 2, 4, or 6 days after bupivacaine injection. Rats were heat stressed by immersion in water (42 ± 1°C) for 30 minutes 24 hours after bupivacaine injection and every other day thereafter. Results: No adult MyHC isoforms were observed after 2 days, whereas some fibers expressed only fast MyHC after 4 days. Heat stress increased fast and slow MyHC in regenerating fibers after 6 days. Regenerating fibers expressing only slow MyHC were observed only in heat‐stressed muscles. Bupivacaine injection increased the number of Pax7+ and MyoD+ satellite cells in regenerating fibers, more so in heat‐stressed rats. Conclusion: The results indicate that heat stress accelerates fast‐to‐slow MyHC phenotype conversion in regenerating fibers via activation of satellite cells. Muscle Nerve 52 : 1047–1056, 2015 相似文献