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目的 研究有活性的激酶C受体(RACK1)在食管鳞癌组织中的表达水平及其临床意义.方法 Western印迹检测食管鳞癌细胞中RACKI的表达.免疫组织化学方法检测组织芯片中113例食管鳞癌组织及其配对的癌旁正常食管黏膜中RACKl和Ki67蛋白的表达水平.将病例按年龄、性别、肿瘤部位、吸烟与否、分化程度和TNM分期进行分组,比较不同组别中RACKl表达水平的差异.结果 RACKl在食管鳞癌组织中的表达低于癌旁正常食管黏膜,差异具有统计学意义(x2=63.363,P<0.01).不吸烟组的肿瘤组织中RACKl高表达率为72.5%(29/40),高于吸烟组的46.6%(34/73)(X2=7.040,P=0.008).TNM l期和Ⅱ期患者的肿瘤组织中RACKl高表达率为63.8%(44/69),高于TNMⅢ期的43.2%(19/44)(0=4.616,P=0.032).Ki67评分越高的肿瘤组织中RACKl的表达越低(x2=8.261,P=0.016).结论 RACKl在食管鳞癌中表达发生下调.食管鳞癌中RACKI的表达与吸烟、TNM分期及Ki67评分具有相关性.
Abstract:
Objective To explore the expression level and clinical significance of receptor for activated C kinase 1(RACKl)in esophageal squamous cell carcinoma(ESCC).Methods Western Blotting was performed to detect the RACKl expression in ESCC cell lines.Immunohistochemistry was conducted to assay the expression of RACKl and Ki67 in tumor tissues and adjacent normal epithelium from 113 ESCC patients in tissue microarray.The relationship between the RACKI level and such clinieopathologic profiles as age,gender,location,smoking,differentiation degree and TNM(tumor,node,metastasis)stage were analyzed.Results The expression of RACKl protein was significantly downregulated in ESCC tissues as compared with the normal adjacent epithelium(X2=63.363,P<0.01).An upregulated expression of RACKl was observed in 72.5%(29/40)ESCC tissues of patients without a smoking history.And it was significantly higher than that in 46.6%(34/73)of patients with a smoking history(x2=7.040,P=0.008).In addition,the rate of up-regulated of RACKl was significantly higher in stage I andⅡgroup(63.8%,44/69)than that in stage Ⅲ group(43.2%,19/44)(x2=4.616,P=0.032).Moreover.the ESCC tissues with a higher Ki67 score showed a lower level of RACKl than that with a lower Ki67 score(x2=8.261,P=0.016).Conclusions The expression of RACKl is down-regulated in ESCC tissues and it is associated with smoking,The expression of RACKl was associated with smoking,TNM staging and Ki67 score of ESCC.  相似文献
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目的:探讨胃癌及癌旁组织中活化的蛋白激酶C受体‐1(RACK1)、类固醇受体辅助活化因子(Src)和 B淋巴细胞瘤‐2(Bcl‐2)基因表达的变化。方法收集华北理工大学附属医院2011年8月1日至2014年2月1日手术切除胃癌及癌旁组织标本各80份,采用免疫组织化学法及蛋白质印迹法(Western blotting )检测RACK1、Src及Bcl‐2蛋白在胃癌及癌旁组织中的表达情况,分析其相关性并结合临床病理学资料进行统计学分析。结果免疫组织化学法与Western blotting检测显示,胃癌组织中RACK1表达阳性率及表达水平均低于癌旁组织,Src与Bcl‐2表达阳性率及表达水平均高于癌旁组织,差异均有统计学意义(P<0.05)。在胃癌组织中,RACK1表达分别与Src、Bcl‐2表达呈负相关(相关系数 r值分别为-0.632、-0.754,P<0.01),Src与Bcl‐2蛋白表达无相关性(r=0.217,P>0.05)。结论胃癌组织中RACK1表达明显降低,而Src与Bcl‐2表达升高。  相似文献
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