Changes and significance of plasma fibrinogen gamma‐chain concentration in preeclampsia patients

ObjectiveTo investigate the plasma fibrinogen gamma‐chain concentration in preeclampsia patients and explore its value in preeclampsia prediction and auxiliary diagnosis.MethodsFollow‐up of pregnant women who regularly attended perinatal care at two hospitals in China was performed, and clinical data and plasma samples were collected at each examination until delivery. The gamma‐chain concentration was detected by Western blotting, and Quantity One Software was used for gamma‐chain grayscale value measurements.ResultsForty‐two patients with preeclampsia and 42 control patients completed the follow‐up. In the control group, the gamma‐chain concentration at 32 weeks of gestation was higher than that at 20 weeks of gestation, but the difference was not statistically significant (p > 0.05). In the experimental group, the gamma‐chain concentration at preeclampsia diagnosis was significantly higher than that at 20 weeks of gestation (p < 0.05). Compared with the control group, the gamma‐chain concentration was higher at 20 weeks of gestation in the experimental group, but the difference was not statistically significant. However, at 32 weeks of gestation and at the time of diagnosis, the gamma‐chain concentration in the experimental group was significantly higher than that in the control group (p < 0.05). At 32 weeks of gestation and at the time of diagnosis, the AUCs from ROC curve analysis of plasma fibrinogen gamma‐chain concentrations were 0.64 and 0.71, respectively.ConclusionPlasma fibrinogen synthesis and degradation were disrupted in preeclampsia patients before and after diagnosis, and gamma‐chain concentration was significantly increased. Plasma fibrinogen gamma chain may be of some value in preeclampsia prediction and auxiliary diagnosis.     

老年心血管病患者潜在性不适当用药及影响因素分析

目的:评价某院老年心血管病患者药物潜在不适当应用情况,并对其影响因素进行分析。方法:抽取某院2015年10月到2016年8月的老年心血管病例,以2014年版STOPP/START标准为依据,对年龄在65岁及以上的出院患者潜在不适当用药(PIM)进行评价,并采用多因素Logistic回归分析确定其影响因素。结果:通过病例筛选纳入514份病例,患者平均年龄(77.44±8.48)岁,平均罹患疾病数(5.93±2.34)种,联合用药总数(16.84±8.89)种;其中涉及2014年版STOPP标准不适当用药190例次,START标准处方遗漏419例次。单因素卡方分析提示,年龄(P=0.022)、罹患疾病数(P=0.041)、联合用药数(P=0.000)、心血管疾病史(P=0.001)及是否接受抗栓治疗(P=0.000)与PIM的发生具有相关性,且差异具有显著性(P<0.05);多因素Logistic回归分析提示稳定型心绞痛(OR=2.543,P=0.025),心房颤动(OR=2.304,P=0.024),冠状动脉粥样硬化(OR=2.659,P=0.005),联合用药数(≥10)(OR=1.040,P=0.026)及接受抗栓治疗(OR=3.037,P=0.007)的患者均会增加潜在不适当用药的风险,采用拟合的Logistic回归预测模型对入选病例预测总的预测正确率为74.5%。结论:老年心血管疾病患者发生潜在不适当用药是稳定型心绞痛、心房颤动、冠状动脉粥样硬化,联合用药数(≥10)及接受抗栓治疗多因素相互影响的结果。基于相关危险因素的PIM预防有助于减少不良事件的发生,促进临床合理用药。     

First trimester uterine artery Doppler,sFlt-1 and PlGF to predict preeclampsia in a high-risk population

Objective: The study aims to evaluate the accuracy of combining uterine artery Doppler (UAD), PlGF and sFlt-1 in the first trimester for preeclampsia screening.

Methods: Prospectively enrolled women at high risk of preeclampsia were included. Transabdominal UAD measurements and serum biomarkers were collected between 11 and 13 weeks of gestation in three university hospitals and in one general hospital. The main outcome was preeclampsia. UAD parameters and biomarker levels among women with preeclampsia were compared with those of women in the unaffected group in univariate and multivariate analyses.

Results: Out of 226 women included from May 2007 to January 2011, 27 (11.9%) women developed preeclampsia. Among women affected by preeclampsia, the lowest pulsatility index was higher (p?=?0.02), bilateral notching was more frequent (p?=?0.01), and PlGF was lower (p?p?=?0.7).

Conclusion: In a high-risk population, PlGF in the first trimester is useful for predicting preeclampsia, but neither sFlt-1 nor any UAD indices improved the prediction of preeclampsia.     

The prognostic role of serum uric acid levels in preeclampsia: A meta‐analysis

This meta‐analysis aims to compare serum uric acid levels among preeclamptic and healthy pregnant women across the various trimesters and provide a summary of the effect size of this biomarker in predicting adverse pregnancy outcomes. MEDLINE, Scopus, CENTRAL, Clinicaltrials.gov, and Google Scholar databases were systematically searched from inception. Observational studies were held eligible if they reported serum uric acid among preeclamptic and healthy pregnant women. Meta‐analysis was conducted regarding uric acid concentration, diagnostic accuracy, and association with perinatal outcomes. The credibility of evidence was appraised using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) framework. The analysis included 196 studies, comprising 39 540 women. Preeclampsia was associated with significantly elevated uric acid levels during the 1st (mean difference [MD]: 0.21 mg/dL, 95% confidence intervals [CI]: 0.06‐0.35) trimester, 2nd (MD: 1.41 mg/dL, 95% CI: 0.78‐2.05) trimester, and 3rd (MD: 2.26 mg/dL, 95% CI: 2.12‐2.40) trimester. Higher uric acid was estimated for severe preeclampsia, eclampsia, and hemolysis, elevated liver enzymes, low platelet syndrome. The sensitivity for adverse perinatal outcome prediction ranged from 67.3% to 82.7% and the specificity from 47.7% to 70.7%. In conclusion, it is suggested that serum uric acid levels are increased in preeclampsia and can be used to predict disease severity and pregnancy complications. Future prospective studies should verify these outcomes, assess the optimal cutoffs, and incorporate uric acid to combined predicting models.     

蛋白质-蛋白质相互作用数据库的研究进展

随着人类蛋白质组学的飞速发展,出现了大量的蛋白质相互作用及其网络数据,因此寻找和预测蛋白质相互作用的生物信息学方法成了研究蛋白质互作必不可少的工具之一,本文主要从生物信息学角度对蛋白质相互作用数据库做一下简单的介绍,包括各数据库的来源、主要功能、应用范围、目前的发展状况及将来的发展方向等方面.     

早期急性重症胰腺炎预后因素分析

Objective Analysis of early risk predict markers within 24h after admission of prognosis in severe acute pancreatitis (SAP). Methods Medical records of 127 patients with sever acute pancreatitis admitted to our hospital within 72h after onset from February 2006 to July 2009 were retrospectively analyzed. These inpatients were grouped into survival group (102 cases) and death group (25 cases). The clinical and laboratory data within 24h after admission were compared between two groups. Results Compared with survival group, patients in death group had significantly difference in heart rate, respiratory, PaO2, pH value, serum calcium, serum kalium, serum creatine, BUN, BE, LDH, serum albumin and APCHE Ⅱscores (P<0.05). Higher APCHE Ⅱ scores after admission was an independent early high risk predicator of death. Conclusions Death group was characterized as severe multiple organ dysfunction and severe internal disturbance. Heart rate, respiratory, PaO2, pH value, serum calcium, serum potassium, serum creatine, BUN, BE, LDH, serum albumin and APCHE Ⅱ scores were early risk predict markers of death in patients with sever acute pancreatitis.     

尿胆红素检测对妊娠肝内胆汁淤积症的预测价值

目的探讨尿胆红素检测对妊娠肝内胆汁淤积症的预测价值。方法 2008年1～6月对成都市妇产科医院门诊133例临床怀疑为ICP的孕妇进行尿胆红素检测和血胆汁酸检测。以血胆汁酸结果为金标准,对两种检测方法的一致性进行评价。结果尿胆红素阳性者其血胆汁酸水平亦高,两者有一致性。结论尿胆红素检测可随机进行,有很高的特异性,可用于ICP的预测。