抗地高辛人源小分子抗体的获取

目的 从大容量噬菌体抗体库中筛选人源性抗地高辛抗体,并构建双体抗体.方法 以固相化的地高辛对构建的大容量噬菌体抗体库进行筛选,3～4轮后挑取克隆用酶联免疫吸附测定方法鉴定其特异性,对抗地高辛阳性抗体克隆进行DNA指纹分析及测序分析.选取活性好的克隆进行改造,构建双体抗体.结果 在抗体库的筛选过程中可见到明显的富集现象,获得了4株可与地高辛特异性结合的人源抗体,经DNA指纹分析及测序分析证明为不同克隆基因,阳性克隆的可变区基因轻链均属于λ第一亚群,重链分别属于第三和第四亚群.选取4号克隆进行基因改造,构建双体抗体表达载体,获得了活性较好的双体抗体.结论 利用噬菌体抗体技术获得了人源性抗地高辛抗体,并改造成应用前景较好的双体抗体,可能为临床诊断及治疗洋地黄类药物中毒提供具有实用价值的人源抗体.     

信息时代高校图书馆信息服务的拓展

论述了信息时代高校图书馆信息服务的优势、模式、特点，探讨了高校图书馆拓展信息服务的策略。     

医院图书馆过刊剔旧工作的思考

分析了医院图书馆过刊剔旧的必要性与可行性,提出了剔旧原则与建立全国性文献剔除网络中心的设想.     

Antigenic and immunogenic mimicry of the HER2/neu oncoprotein by phage-displayed peptides

To recover peptides that antigenically and immunogenically mimic the p185^HER2 oncoprotein, we selected the phage-peptide libraries pVIII-9aa and pVIII-9aa. Cys using murine monoclonal antibodies (mAb) MGr2 and MGr6, directed against two distinct epitopes of the p185^HER2 extracellular domain. Phagedisplayed peptides containing consensus amino acid motifs were recovered and shown to compete specifically for mAb binding on tumor cells that overexpress p185^HER2. The deduced amino acid sequence of the peptides suggests that both epitopes defined by the mAb on p185^HER2 are discontinuous and that hydrophobic interactions are involved in binding with the mAb. A phage clone displaying the GPLDSLFAQ peptide elicited a specific immune response against the p185^HER2 in BALB/c mice, demonstrating that this phage-displayed peptide represents an immunological equivalent of the MGr2 epitope on p185^HER2 and might be used as a substitute for this oncoprotein in in vitro and in vivo immunological studies.     

Time-resolved fluorometric assay for the detection of endostatin in chromatographically separated extracts of natural peptides

We present a heterogeneous non-competitive immunological detection assay for peptide and protein antigens from crude extracts of biological sources. This time-resolved fluoroimmunoassay (TR-FIA) has been designed in a solid-phase mode using 96-well microtiter plates. Using the rare-earth metal europium as a fluorescent marker, a highly sensitive, selective and efficient procedure was developed. This technique prevents from interferences of intrinsic protein fluorescence which is highly important for antigen measurement in complex matrices. The TR-FIA has been applied for the detection of circulating forms of the potential anti-tumor agent endostatin, a C-terminal fragment of collagen XVIII, and its close homolog collagen XV (restin) from hemofiltrate. Endostatin was detected with a limit of detection of 3 ng (150 fmol/well) and a broad dynamic range from 10-1000 ng/well.     

A versatile shuttle cosmid vector for the efficient construction of genomic libraries and for the cloning of fungal genes

A shuttle cosmid vector, pANsCos1, has been constructed for Escherichia coli and filamentous fungi. This vector contains two cos sequences separated by a single XbaI restriction site. pANsCos1 allows the efficient construction of representative genomic libraries from as little as 15–20 g of genomic DNA. Due to the presence of a functional hygromycin B phosphotransferase gene (hph) transformation of fungal protoplasts with pANsCos1, or derivatives of it, results in the formation of hygromycin B-resistant transformants. The T7 and T3 RNA polymerase promoter sequences flanking the cloning site, in combination with two adjacent NotI sites facilitate genomic walking and the rapid construction of restriction maps of cloned inserts.Dedicated to Professor Dr. K. Esser on the occasion of his 70th birthday     

中医院校图书馆参考咨询员知识结构分析

从医、教、研人员对中医古籍图书的需求、对中医古代专题资料的需求、对现代中医药期刊文献的需求、对特种中医药文献的需求、对中医药数据信息的需求、对中医药课题研究的定性需求等实例出发，对中医院校图书馆参考咨询员的知识结构进行了分析。     

学习型图书馆的理论与实践

以彼得.圣吉博士的学习型组织作为学习型图书馆的理论基础,探讨了学习型社会对图书馆和图书馆员的要求和建立学习型图书馆的必要性,阐述了学习型图书馆的特征,以及如何建立学习型图书馆。     

以馆藏资源助力地域文化符号搭建文旅融合底蕴平台

地域文化符号是地方历史文化发展的载体,也是地域文明的象征。图书馆作为地域信息及文献资源的收藏地,在地域文化发展中起着至关重要的作用,在当前经济社会发展的背景下,也是文旅融合发展的重要组成部分。本文以图书馆助力地域文化符号搭建文旅融合底蕴平台为出发点,通过分析找准地域文化符号的意义和方法,探究图书馆在充分利用馆藏资源的基础上,找准文旅融合的契合点,厚积地域文化底蕴的方式和路径,通过进一步融合现代发展元素,打造文化旅游特色品牌项目,使文旅产业成为带动地域经济发展的"动力产业"。