Hydrocellular foam dressing increases the leptin level in wound fluid

Hydrocellular foam dressing (HCF) absorbs excessive wound fluid, which contains various cytokines and growth factors, and ensures a moist environment to promote wound healing. However, the molecular mechanisms underlying the wound fluid component changes induced by HCF are poorly understood. In the present study, we examined the effect of HCF on wound healing and the associated regulatory mechanisms in relation to variations in cytokine levels in the wound fluid. We created full‐thickness wounds on the dorsolateral skin of rats and collected the resulting wound fluid samples. HCF was immersed in a plate containing the wound fluids. HCF was then removed and the excess wound fluid remaining in the plate was examined by cytokine array and enzyme‐linked immunosorbent assay. We also used a rat model and human dermal fibroblast cultures to examine the effect of wound fluid component changes during the wound healing process. Upon treatment with HCF, leptin levels were upregulated in the wound fluid. Fibroblast proliferation was enhanced and the effect was suppressed in the presence of leptin antagonist. In our in vivo model, HCF increased wound contraction compared with film dressings and this positive effect of HCF was suppressed by addition of leptin antagonist. Our results suggest that dermal fibroblast proliferation is upregulated by HCF due to increased leptin level at the wound surface, and these effects promote wound healing. We believe that the present study contributes to furthering the understanding of the mechanisms underlying the effects of HCF‐induced wound healing.     

子痫前期血清脂联素反常升高与瘦素水平改变的相关性

目的探讨血清脂联素、瘦素和可溶性瘦素受体水平在子痫前期患者中的变化及其意义。方法采用酶联免疫吸附试验方法检测38例子痫前期患者和42例正常妊娠（对照组）血清脂联素、瘦素和可溶性瘦素受体水平，并计算游离瘦素指数值（瘦素／可溶性瘦素受体）。结果子痫前期患者血清脂联素、瘦素和游离瘦素指数分别为1691，7μg／ml、37．5ng／ml和0．95，明显高于对照组（分别为689，4μg／ml、19．3ng／ml和0．49），但两组别间可溶性瘦素受体水平则未见明显差异（分别为35．0ng／ml和42，2ng／ml）。在脂联素和瘦素、可溶性瘦素受体及游离瘦素水平间并未见明显相关，子痫前期患者脂联素、瘦素和游离瘦素的ROC曲线下面积均低于0.5。结论子痫前期患者血清脂联素水平存在明显的反常升高，瘦素的生物利用度也明显提高，可能与子痫前期的发生发展有关。