排序方式: 共有46条查询结果,搜索用时 15 毫秒
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摘 要 目的:建立HPLC色谱法测定甘草流浸膏中甘草酸铵的含量。方法: 采用HPLC色谱法在Daminsil C18(250 mm×4.6 mm,5 μm) 色谱柱上梯度洗脱分离,乙腈-0.1%磷酸为流动相,梯度洗脱,检测波长为250 nm,流速为1.0 ml·min-1,柱温为30℃。结果:甘草酸铵在0.046 8~0.234 0 mg·mL-1范围内线性关系良好,平均回收率为97.83%,RSD为0.39%(n=9)。结论: 该方法准确、灵敏度高、重现性好、简便可行,可作为含有甘草流浸膏制剂的质量控制方法。 相似文献
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目的研究甘草黄柏洗剂的提取工艺。方法采用高效液相色谱(HPLC)法,以小檗碱的提取率为评价指标,分别对甘草黄柏合煎液及合并液进行测定。结果小檗碱含量在甘草黄柏合煎液与合并液之间存在明显差异。结论在制定甘草黄柏洗剂的提取工艺时,以黄柏与甘草分煎后再合并,有利于黄柏中小檗碱的充分利用。 相似文献
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甘草活性成分抗呼吸道合胞病毒作用 总被引:5,自引:0,他引:5
目的研究甘草抗病毒活性成分(GX)体外抗呼吸道合胞病毒(RSV)的作用。方法采用中性红实验和观察细胞病变(CPE)法,以利巴韦林为阳性药,测定GX对RSV的抑制作用。结果GX的半数中毒浓度(TC50)为460.00μg/ml,半数有效抑制浓度(EC50)为23.53μg/ml,治疗指数(TI)为19.55;GX在RSV感染Hela细胞2,4,6,8,10 h后给药,对RSV复制均有明显的抑制作用(P〈0.01);且GX对RSV有中和作用。结论GX在体外对RSV复制有明显的抑制作用。 相似文献
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抗感冒颗粒的薄层色谱和含量测定研究 总被引:1,自引:0,他引:1
目的建立抗感冒颗粒的质量标准。方法采用薄层色谱法对处方中黄芩、甘草、桔梗进行鉴别;用高效液相色谱法测定制剂中黄芩苷的含量。采用AichromBond-AQ-C18色谱柱(4.6mm×150mm,5μm);流动相:甲醇-水-磷酸(43∶57∶0.2);流速:1.0mL/min;柱温:30℃;检测波长:280nm;进样量:10μL。结果薄层色谱专属性强;黄芩苷的线性范围为0.0496~0.2976μg,r=0.9997,平均加样回收率为101.80%,RSD=1.86%(n=6)。结论该方法简便、准确,重现性好,可作为抗感冒颗粒的质量控制标准。 相似文献
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目的完善藿香祛暑软胶囊的质量标准。方法采用薄层色谱法分别对广藿香、白芷、紫苏叶、苍术、法半夏、茯苓和甘草进行鉴别。结果薄层色谱上均检出有关药材的特征斑点,薄层色谱斑点显色清晰,且阴性对照无干扰。结论本方法专属性强,操作简单,重现性好,可作为藿香祛暑软胶囊的质量控制。 相似文献
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目的研究甘草水煎剂对体外培养的变异链球菌的抗菌活性。方法取粒径为0.2~3.2 mm的甘草颗粒加去离子水煎煮后过滤,滤液为实验用甘草水煎剂。采用液体稀释法测定甘草水煎剂对变异链球菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。在不同浓度甘草水煎剂中培养变异链球菌,分别于培养0﹑3﹑7﹑12﹑23、40 h时测定细菌悬液光密度值和培养液pH值,绘制变异链球菌的生长曲线和产酸曲线。结果甘草水煎剂对变异链球菌的MIC为50 mg·mL-1,浓度小于等于100 mg·mL-1的甘草水煎剂对变异链球菌无杀菌作用。甘草水煎剂对变异链球菌生长的抑制作用随浓度的增加而增强。甘草水煎剂对变异链球菌的产酸有一定的抑制作用,在培养12 h时抑制作用最强。结论甘草水煎剂对体外培养的变异链球菌生长和产酸均有抑制作用。 相似文献
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Xuehong Nöst Eva-Maria Pferschy-Wenzig Xiao-Tong Yu Min Li Xiao-Lin Tong Rudolf Bauer 《World Journal of Traditional Chinese Medicine》2021,(1)
Objective: Gegen Qinlian decoction(GQD) is a classical traditional Chinese medicine formulation which has been used for almost 2000years. At Guang’anmen
Hospital,Beijing, a modified GQD version (mGQD) with seven instead offour herbal ingredients has been applied to treatType 2 diabetes. Quality control
is a crucial prerequisite for the therapeutic application of herbal medicines. For the identification of products derived from classical GQD, the Chinese
Pharmacopeia requiresthe analysis of only three marker compounds. Because mGQD is a more complex mixture containing seven herbs and hundreds of
constituents,thepharmacopoeiamethodforGQDisinadequate.Materials and Methods: Amore comprehensive characterization of the formula''s constituents
has been developed using ultra?high?performance liquid chromatography?diode array detection (UHPLC?DAD)?Q?Exactive?mass spectrometry (MS)
in electrospray ionization positive and negative mode. Moreover, a new method for the fingerprint analysis of mGQD via high-performance thin-layer
chromatography (HPTLC) has been established. Results: Altogether, 91 compounds have been assigned to their originating plants and 84 substances were
identified either by comparison with authentic references or with data from the literature. The HPTLC method is based on the application of two different
mobile phases and is able to detect both lipophilic and hydrophilic constituents of mGQD. Conclusions:ThemodifiedGQDwasextensivelycharacterizedby
UHPLCcombined with DAD and Q?Exactive Orbitrap high?resolution MS detection, leading to the assignment and identification of compounds present in
the decoction.In addition, a newmethod forthefingerprint analysis ofthemGQDusingHPTLCwas established,which allowsfast and simple identification
of the herbal ingredients in the mixture. 相似文献