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1.
目的 探讨溴结构域蛋白4(bromodomain protein 4,BRD4)、线粒体单链DNA结合蛋白(single-stranded DNA binding protein,SSBP1)与原钙黏附蛋白17( proto-cadherin 17,PCDH17)诊断早期胃癌的临床价值。 方法 收集胃癌组织50例,癌前病变组织50例、正常胃黏膜组织50例,应用免疫组织化学染色法分别测定组织标本中BRD4、SSBP1及PCDH17蛋白的表达,分析不同组织样本中BRD4、SSBP1及PCDH17的表达情况,分别采集3组血液标本,检测3组相关血清指标,应用ROC曲线分析BRD4、SSBP1及PCDH17诊断胃癌的价值。 结果 胃癌组织中BRD4和SSBP1呈高表达,而PCDH17呈低表达。3组组织样本中BRD4、SSBP1及PCDH17的阳性率比较差异有统计学意义(P<0.05);3组血清癌抗原199(carbohydrate antigen,CA199)、血清胃蛋白酶原(pepsinogen,PG)Ⅰ、PGⅡ、胃泌素17(gastrin-17,G-17)水平及幽门螺杆菌(helicobacter pylori,HP)阳性率差异有统计学意义(P<0.05);胃癌组织组患者血清CA199、PGⅠ、G-17水平及HP阳性率明显高于癌前病变组织和正常胃黏膜组织组,而PGⅡ水平低于癌前病变组织和正常胃黏膜组织组,差异有统计学意义(P<0.05);临床分期为Ⅲ期~Ⅳ期、发生淋巴结转移的胃癌组织中BRD4、SSBP1、PCDH17的阳性率比较差异有统计学意义(P<0.05);BRD4+SSBP1+PCDH17联合诊断的敏感度和特异度均高于单一指标。 结论 联合检测BRD4、SSBP1与PCDH17有利于胃癌的鉴别诊断。  相似文献   
2.
目的研究免疫学检验联合检测诊断类风湿关节炎(RA)的临床价值。方法40例进行体检的健康人员作为参照组,同期40例类风湿关节炎患者作为研究组。两组受检者均接受免疫学检验联合检测,分析检验结果。比较两组类风湿因子(RF)、抗环瓜氨酸多肽抗体(抗CCP)、抗角蛋白抗体(AKA)阳性检出率,免疫球蛋白G(IgG)、补体C3、补体C4水平。结果研究组的RF、抗CCP、AKA阳性检出率分别为85.00%、62.50%、85.00%,均高于参照组的2.50%、5.00%、0,差异具有统计学意义(P<0.05)。研究组的IgG为(15.64±4.71)g/L、补体C3为(0.68±0.25)g/L、补体C4为(0.14±0.05)g/L,参照组的IgG为(10.47±3.08)g/L、补体C3为(1.23±0.31)g/L、补体C4为(0.29±0.17)g/L。研究组的IgG高于参照组,补体C3、补体C4低于参照组,差异具有统计学意义(P<0.05)。结论采用免疫学检验联合检测诊断类风湿关节炎的准确率较高,临床可以将其作为类风湿关节炎诊断的有效方案,为患者的疾病治疗提供参考。  相似文献   
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ObjectiveAeromonas has recently been recognized as an emerging human pathogen. Aeromonas-associated diarrhea is a phenomenon occurring worldwide. This study was designed to determine the prevalence, genetic diversity, antibiotic resistance, and pathogenicity of Aeromonas strains isolated from food products in Shanghai.MethodsAeromonas isolates (n = 79) collected from food samples were analyzed using concatenated gyrB-cpn60 sequencing. The antibiotic resistance of these isolates was determined using antimicrobial susceptibility testing. Pathogenicity was assessed using β-hemolytic, extracellular protease, virulence gene detection, C. elegans liquid toxicity (LT), and cytotoxicity assays.ResultsEight different species were identified among the 79 isolates. The most prevalent Aeromonas species were A. veronii [62 (78.5%)], A. caviae [6 (7.6%)], A. dhakensis [3 (3.8%)], and A. salmonicida [3 (3.8%)]. The Aeromonas isolates were divided into 73 sequence types (STs), of which 65 were novel. The isolates were hemolytic (45.6%) and protease-positive (81.0%). The most prevalent virulence genes were act (73.4%), fla (69.6%), aexT (36.7%), and ascV (30.4%). The results of C. elegans LT and cytotoxicity assays revealed that A. dhakensis and A. hydrophila were more virulent than A. veronii, A. caviae, and A. bivalvium. Antibiotic resistance genes [tetE, blaTEM, tetA, qnrS, aac(6)-Ib, mcr-1, and mcr-3] were detected in the isolates. The multidrug-resistance rate of the Aeromonas isolates was 11.4%, and 93.7% of the Aeromonas isolates were resistant to cefazolin.ConclusionThe taxonomy, antibiotic resistance, and pathogenicity of different Aeromonas species varied. The Aeromonas isolates A. dhakensis and A. hydrophila were highly pathogenic, indicating that food-derived Aeromonas isolates are potential risks for public health and food safety. The monitoring of food quality and safety will result in better prevention and treatment strategies to control diarrhea illnesses in China.  相似文献   
5.
IntroductionIdiopathic pulmonary fibrosis (IPF) is progressive and irreversible. Some discrepancies about IPF staging exists, especially in mild phases. Forced vital capacity (FVC) higher than 80% has been considered early or mild IPF even for the design of clinical trials.MethodsSpanish multicentre, observational, retrospective study of IPF patients diagnosed between 2012 and 2016, based on the ATS/ERS criteria, which presented FVC greater or equal 80% at diagnosis. Clinical and demographic characteristics, lung function, radiological pattern, treatment, and follow-up were analyzed.Results225 IPF patients were included, 72.9% were men. The mean age was 69.5 years. The predominant high-resolution computed tomography (HRCT) pattern was consistent usual interstitial pneumonia (UIP) (51.6%). 84.7% of patients presented respiratory symptoms (exertional dyspnea and/or cough) and 33.33% showed oxygen desaturation below 90% in the 6 min walking test (6MWT). Anti-fibrotic treatment was initiated at diagnosis in 55.11% of patients. Median FVC was 89.6% (IQR 17) and 58.7% of patients had a decrease of diffusion lung capacity for carbon monoxide (DLCO) below 60% of theoretical value; most of them presented functional progression (61.4%) and higher mortality at 3 years (20.45%). A statistically significant correlation with the 3-years mortality was observed between DLCO <60% and consistent UIP radiological pattern.ConclusionsPatients with preserved FVC but presenting UIP radiological pattern and moderate–severe DLCO decrease at diagnosis associate an increased risk of progression, death or lung transplantation. Therefore, in these cases, preserved FVC would not be representative of early or mild IPF.  相似文献   
6.
间隙连接蛋白(Cx)在保证细胞间物质信息传递及维持皮肤屏障稳态方面发挥着重要作用,其基因突变,甚至表达水平异常均会引起多种疾病,严重影响患者生活质量。在编码人类Cx家族的21个基因中,与Cx基因突变相关的临床伴随疾病至少有14种。其中,Cx43分布最广,不仅在大多数器官组织中均有报道,也是伤口愈合、皮肤角质化,以及皮肤肿瘤发展等重要生理病理过程中的关键调控节点。本文总结了近年来Cx43基因(GJA1)在皮肤屏障中的作用、GJA1基因突变相关皮肤疾病及其潜在致病机制这几个快速发展领域中的研究成果,以期为Cx43临床伴发疾病防治及相关研究提供参考。  相似文献   
7.
目的 运用标准化患者法评估四川农村地区基层医生不稳定型心绞痛和2型糖尿病两种慢性病诊断准确性现状,探讨基层医生两种慢性病诊断准确性的主要影响因素,为提升基层医生两种慢性病诊断准确性提供科学依据。方法 采用多阶段随机整群抽样方法,抽取四川省自贡市5个区/县50个乡镇100个村为研究现场,以调查当日在岗的全科及内科医生作为研究对象。共进行两轮数据采集,第1轮采集样本乡镇卫生院和村卫生室医生的基本信息;第1轮调查完成1个月后,运用标准化患者法开展第2轮调查,收集农村基层医生对不稳定型心绞痛和2型糖尿病诊断结果信息。运用Logistic回归分析农村基层医生不稳定型心绞痛和2型糖尿病诊断准确性的影响因素。结果 共纳入172名农村基层医生,完成186次标准化患者访问,正确诊断率为48.39%。其中不稳定型心绞痛的正确诊断率为18.68%(17/91),2型糖尿病的正确诊断率为76.84%(73/95)。Logistic回归分析显示,具有执业医师资质的农村基层医生更有可能做出正确诊断(OR=4.857,95%CI=1.076~21.933,P=0.040)。农村基层医生在诊断过程中涉及的必要问诊和检查条目越多,做出正确诊断的概率越高(OR=1.627,95%CI=1.065~2.485,P=0.024)。与不稳定型心绞痛相比,农村基层医生对2型糖尿病做出正确诊断的可能性更高(OR=6.306,95%CI=3.611~11.013,P<0.001)。结论 四川农村基层医生不稳定型心绞痛和2型糖尿病诊断准确性整体较差,建议以基层医生慢性病诊断过程质量改善为突破口,提升基层医生执业水平,进而提高慢性病诊断准确性。  相似文献   
8.
目的探讨朗格汉斯细胞组织细胞增生症的临床病理特征。 方法回顾性分析2016年1月至2021年9月泰安市中心医院病理科确诊的5例朗格汉斯细胞组织细胞增生症患者的临床资料,分析其病理形态学特点及免疫表型。 结果男性2例,女性3例;3例累及骨组织,1例累及腭部软组织,1例累及淋巴结;形态学均表现为肿瘤细胞呈圆形或卵圆形,有核沟,细胞质轻度嗜酸性,背景为数量不等的嗜酸性粒细胞、组织细胞、小淋巴细胞及中性粒细胞。免疫表型方面,肿瘤细胞一致性表达CD4、CD1α及S-100。 结论朗格汉斯细胞组织细胞增生症是一种少见的朗格汉斯细胞克隆性增生性病变,免疫组织化学表达稳定,选择合适的免疫组织化学抗体并结合病理形态学特征方能正确诊断。  相似文献   
9.
AIM: To investigate the clinical characteristics and genetic features of a Bietti crystalline dystrophy (BCD) proband in a Chinese family. METHODS: A Chinese female diagnosed with BCD complicated by bilateral choroidal neovascularization (CNV) and her parents underwent complete ophthalmic examinations, including fundus autofluorescence (AF), fundus photography (FP), fundus fluorescein angiography (FFA), visual field testing, full-field electroretinography (ERG), optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA). The sequencing of the CYP4V2 gene was performed to the whole family. RESULTS: Bilateral tiny glittering crystal-like deposits and differing extent of atrophy of the retinal pigment epithelium (RPE) were found in the posterior pole of her fundus. The diffuse hypo-fluorescence shown on AF images and window defects shown on FFA both indicated the atrophy of the RPE and choriocapillaris. OCT showed the thinning of the RPE and choriocapillaris layer, ellipsoid zone (EZ) band defect and CNV in both eyes. OCTA images proofed bilateral type 2 CNV. The visual field test showed central and paracentral scotoma. ERG showed a slightly decreased b-wave in scotopic ERG. Gene sequencing identified three mutations of the CYP4V2 gene, c.802_807del, c.810delT, and c.1388G>A. The mutation c.1388G>A was a novel substitution mutation. CONCLUSION: The novel mutation c.1388G>A may be a possible cause that could induce the clinical phenotype of BCD.  相似文献   
10.
Pancreatic cancer (PC) is a cancer of the digestive system, and pancreatic ductal adenocarcinoma (PDAC) accounts for approximately 90% of all PC cases. Exosomes derived from PDAC (PDAC-exosomes) promote PDAC development and metastasis. Exosomes are nanoscale vesicles secreted by most cells, which can carry biologically active molecules and mediate communication and cargo transportation among cells. Recent studies have focused on transforming exosomes into good drug delivery systems (DDSs) to improve the clinical treatment of PDAC. This review considers PDAC as the main research object to introduce the role of PDAC-exosomes in PDAC development and metastasis. This review focuses on the following two themes: (a) the great potential of PDAC-exosomes as new diagnostic markers for PDAC, and (b) the transformation of exosomes into potential DDSs.  相似文献   
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