斑蝥黄微胶囊在家兔体内的药代动力学

目的:研究斑蝥黄微胶囊在家兔体内的药代动力学。方法:家兔单剂量灌胃给予斑蝥黄微胶囊后,在不同时间点经后肢静脉采血,用LC-MS法测定血浆中斑蝥黄质量浓度。药代动力学参数运用DAS2.1软件处理。结果:家兔灌胃给予斑蝥黄微胶囊后斑蝥黄浓度-时间曲线符合二室模型,主要药代动学参数Tmax=300 min,Cmax=(75.761±0.739)μg·L-1,t1/2z=(527.408±208.401)min,AUC(0-t)=(23 057.221±1 582.299)μg·L-1·min-1,Vz/F=(1 569.453±258.575)L·kg-1。结论:家兔灌胃给予斑蝥黄微胶囊后,斑蝥黄在体内消化吸收缓慢、体内分布广泛,说明斑蝥黄微胶囊具有稳定和缓释的特点。     

Canthaxanthin protects human sperm parameters during cryopreservation

Different antioxidants have been introduced to reduce oxidative stress during the cryopreservation. The main goal of this study was to evaluate the effects of canthaxanthin on human sperm parameters during the freeze‐thaw process. This study was performed on 25 normozoospermic semen samples dividing into five groups including 0, 0.1, 1, 10, and 25 µM of canthaxanthin. The prepared spermatozoa were cryopreserved by rapid freezing technique. Sperm motility, viability (eosin‐nigrosin), morphology (Papanicolaou), acrosome reaction (double staining), DNA denaturation (acridine orange), chromatin packaging (aniline blue and toluidine blue), and DNA fragmentation (sperm chromatin dispersion test) were evaluated before freezing and after thawing. All sperm parameters after thawing significantly were decreased compared to before freezing. Twenty‐five micromolar canthaxanthin could significantly improve the progressive and total motility, viability, normal morphology, chromatin packaging, acrosome integrity and DNA denaturation and fragmentation. Ten micromolar canthaxanthin significantly improved total motility, viability, normal morphology, chromatin packaging, acrosome integrity and DNA denaturation and fragmentation. Whereas, in 1 µM group, there were significant differences only in improvement of acrosome integrity, chromatin packaging (toluidine blue) and DNA denaturation and fragmentation. But, in 0.1 µM group, there were no significant differences in any of measured parameters. It seems that canthaxanthin ameliorates detrimental effects of cryopreservation on human sperm parameters.     

角黄素改善H2O2对成骨细胞损伤的影响

目的:在H2O2胁迫下,研究角黄素增强成骨细胞抗自由基损伤的作用.方法:用H2O2作用M3T3-E1成骨细胞,建立自由基损伤细胞模型.培养的成骨细胞分为对照组、模型组、角黄素低剂量组(1×10-8 mol/L)、角黄素中剂量组(1×10-7 mol/L)和角黄素高剂量组(1×10-6 mol/L).测定不同处理组细胞活力、超氧化物歧化酶(superoxide dismutase,SOD)活性、活性自由氧(reactive oxygen species,ROS)含量、脂质过氧化物(lipid oxygen,LPO)含量,同时利用荧光偏振法测定细胞膜流动性.结果:模型组细胞活力、SOD活性、细胞膜流动性均比不同角黄素组有显著降低(P＜0.01),而ROS含量、LPO含量均比不同角黄素组有显著升高(P＜0.01).结论:H2O2摄入会导致大鼠成骨细胞的氧化损伤,而角黄素可以预防或降低此类损伤对细胞的影响.     

角黄素对D-半乳糖老龄大鼠骨质的影响

 目的研究角黄素对D-半乳糖致衰老大鼠骨质的影响。方法用D-半乳糖注射Wistar雄性大鼠5个月,建立衰老模型,对青年组、模型组、角黄素组和正常老龄对照组进行骨常规参数测定及比较。结果D-半乳糖组骨密度、骨结构力学参数、生物力学参数、骨钙、镁、锰及羟脯氨酸含量均比青年组有显著降低(P<0.01),而骨磷含量、骨和血清碱性磷酸转移酶均比青年组有显著升高(P<0.01),这些变化与正常老龄对照组的变化趋势一致,并比后者变化得更显著。相比之下,角黄素组上述参数指标均比模型组呈现显著差异(P<0.01)。结论长期大量摄入D-半乳糖导致大鼠衰老,同时可能诱发其发生骨质变化,而角黄素可以预防或降低此类致老骨质变化的发生。     

角黄素对D-半乳糖致衰老大鼠抗氧化功能影响

目的研究角黄素对D-半乳糖致衰老大鼠抗氧化能力的影响。方法用D-半乳糖注射Wistar雄性大鼠5个月,建立衰老模型。对致衰老模型组、角黄素组肝脏、肾脏、心脏、脑和前列腺等脏器丙二醛（MDA）含量、超氧化物歧化酶（SOD）、谷胱甘肽过氧化酶（GSH-Px）活性进行测定及比较。结果角黄素组各脏器MDA含量均比模型组降低,差异有统计学意义（P〈0.01）,而SOD、GSH-Px酶活性比模型组升高,差异有统计学意义（P〈0.01）。结论摄入适量角黄素可有效增强大鼠机体抗氧化能力,从而延缓D-半乳糖诱发的大鼠衰老。