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排序方式: 共有832条查询结果,搜索用时 31 毫秒
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Milan Sýs Atripan Mukherjee Granit Jashari Vojtch Adam Amir M. Ashrafi Miroslav Novk Luk Richtera 《Materials》2021,14(1)
In this article, construction of amperometric sensor(s) based on screen-printed carbon electrodes covered by thin layers of two types of carbon nanomaterials serving as amplifiers, and containing [Cu(bipy)2Cl]Cl∙5H2O complex is reported. Their performance and biomimetic activity towards two selected neurotransmitters (dopamine and serotonin) was studied mainly using flow injection analysis (FIA). The important parameters of FIA such as working potential, flow rate, and pH were optimized. The mechanism of the catalytic activity is explained and experimentally confirmed. It reveals that presence of hydrogen peroxide plays a crucial role which leads to answer the title question: can presented complex really be considered as a tyrosinase biomimetic catalyst or only as a redox mediator? 相似文献
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A particularly facile synthetic route to mussel‐inspired oligo(ethylene glycol) catecholates is described, yielding high purity materials with minimal purification. The oligocatecholates show remarkable thermal and rheological properties for their actual chain length, suggesting the operation of non‐covalent interactions between their l ‐DOPA‐bearing chain ends. This end‐group effect is more pronounced in the shorter oligomers (M n of ethylene glycol chain of 200 and 400 Da), where the end‐group density is higher. Various surfaces (glass, stainless steel) are modified with the oligocatecholates, using a dip‐coating approach from dilute aqueous solutions. Water contact angle measurements and X‐ray photoelectron spectroscopy confirm the presence of a hydrophilic surface coating layer. The potential of these materials for application as antifouling coatings and viscosity modifiers is highlighted. 相似文献
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聚酰胺—胺树枝状聚合物(PAMAM)作为一种新型超支化大分子聚合物,因其出色的化学和生物学特性,一直被众学者称之为“人工蛋白”。 PAMAM的特点是存在内部空腔,并含有大量的反应性末端基团,这些结构使得PAMAM可以被用作仿生大分子,模拟天然有机基质在牙体组织表面进行仿生矿化,即PAMAM作为有机模板调控矿物质成核以及晶体的生长,以此来建造比传统的异体材料更加理想的牙体修复材料。本文就 PAMAM 诱导牙体硬组织仿生矿化的研究进展作一综述。 相似文献
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Dentine sialoprotein (DSP) represents 5-8% of all non-collagenous proteins present in the tooth, but, together with dentine phosphoprotein, has been shown to be vital for correct tooth formation. Recently, the existence of a highly glycosylated form of porcine DSP has been reported and it was shown to possess glycosaminoglycan (GAG) chains. The current investigation confirms that this is also the case for bovine DSP and has further characterized these carbohydrates. Dentine sialoprotein was purified from bovine dentine extracts by anion exchange chromatography and identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), western blotting, and mass spectroscopy. An increase in molecular mass was observed, from 120 kDa to greater than 250 kDa, with a corresponding rise in anionic strength. Cellulose acetate electrophoresis and western blotting indicated the presence of chondroitin sulfate GAG chains within these dentine fractions. Further examination using sequential digestion with chondroitinase AC and N-glycosidase cleaved the samples first to 95 kDa and then to 80 kDa, respectively, confirming a high level of glycosylation. These results support the classification of bovine DSP as a proteoglycan, and that the carbohydrate substitutions may contribute to the functional properties of DSP. 相似文献
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目的以牙骨质附着蛋白为分化指标,分析牙周韧带细胞在纯钛表面的分化趋势,并探讨诱导矿化对牙周韧带细胞牙骨质附着蛋白表达的影响。方法第三代牙周韧带细胞在商用纯钛表面接种后,分别进行常规培养和矿化液诱导矿化培养,通过免疫荧光原位检测牙骨质附着蛋白的表达,并比较诱导矿化对牙骨质附着蛋白表达的影响。结果牙周韧带细胞在纯钛表面初期呈条纹状生长,常规培养和诱导矿化培养务件下均能表达牙骨质附着蛋白,诱导矿化对牙骨质蛋白的表达无明显影响。结论在常规培养务件和诱导矿化培养条件下,纯钛表面生长的牙周韧带细胞能高度表达牙骨质附着蛋白,提示纯钛表面体外培养的牙周韧带细胞具有形成牙骨质的分化趋势,从而可为种植体周构建牙周韧带结构提供在种植体侧的锚着点。 相似文献
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Eduardo Moreira da Silva Alice Gonçalves PenelasMichele Silveira Simão Jaime Dutra Noronha FilhoLaiza Tatiana Poskus José Guilherme Antunes Guimarães 《Journal of dentistry》2010
Objectives
To analyse the influence of the degree of dentine mineralization on the pulp chamber temperature increase during composite light-activation.Methods
Dentine discs (2 mm thick) obtained from recently extracted teeth or those with extensive dentine sclerosis were analysed by FT-IR spectrometry in order to choose the two discs with the greatest difference in the degree of mineralization. A model tooth was set up with the dentine discs between a molar with the pulp chamber exposed and a crown with a standardized class II cavity. A K-type thermocouple was introduced into the molar root until it came into contact with the dentine discs and the cavity was filled with P60 resin composite. The temperature rise was measured for 120 s after light-activation began: Standard (S) 600 mW/cm2/40 s; Ramp (R) 0 → 800 mW/cm2/10 s + 800 mW/cm2/10 s; Boost (B) 850 mW/cm2/10 s and LED (L) 1.300 mW/cm2/40 s (n = 10). The same protocol was repeated after grinding the dentine discs to 1.0 and 0.5 mm thickness.Results
The temperature increase was significantly higher in dentine with high degree of mineralization (p < 0.05). With respect to the dentine thickness, the following result was found: 2 mm < 1 mm < 0.5 mm (p < 0.05). The light-activation mode also presented significant difference as follows: S > R = L > B (p < 0.05).Conclusions
The higher the degree of dentine mineralization the greater the increase in pulp chamber temperature. The temperature increase was influenced by the light-polymerization mode and dentine thickness. 相似文献9.
牙本质基质蛋白1基因反义核酸对MDPC-23细胞碱性磷酸酶活性和矿化能力的影响 总被引:1,自引:0,他引:1
目的 :观察牙本质基质蛋白 1(dentalmatrixprotein ,DMP1)基因反义寡核苷酸 (AS -ODN)对成牙本质细胞系MDPC -2 3细胞碱性磷酸酶 (ALPase)活性和矿化能力的影响 ,深入研究成牙本质细胞的分化和牙本质形成的机制。方法 :以稳定表达DMP1的MDPC -2 3细胞为靶细胞、不同浓度DMP1反义核酸为阻断剂 ,观察不同作用时间对MDPC -2 3细胞ALPase活性的影响。用Westernblot法检测 10 μmol/LAS -ODN不同作用时间细胞DMP1表达情况 ,并观察连续作用 10d对该细胞矿化能力的影响。结果 :与正常组和S -ODN组比较 ,不同浓度的AS -ODN能够降低MDPC -2 3细胞ALPase活性 ,其中 10 μmol/LAS -ODN降低ALPase活性最为显著 (作用 5d时OD值最低 ,为 0 .3 3 78± 2 .0E)。Westernblot法检测到DMP1在MDPC -2 3细胞的表达在 10 μmol/LAS -ODN加入 12h后减弱 ,2 4h后完全阻断。此浓度AS -ODN连续作用细胞 10d后 ,vonKossa染色显示细胞中出现明显的钙盐沉积 ,矿化结节的数量和大小明显少于对照组。结论 :DMP1反义核酸能够降低MDPC -2 3细胞AL Pase活性和矿化能力 ,提示DMP1参与调节成牙本质细胞矿化过程 ,可能具有启动牙本质矿化的作用。 相似文献
10.
Zizelmann C Schoen R Metzger MC Schmelzeisen R Schramm A Dott B Bormann KH Gellrich NC 《Clinical oral implants research》2007,18(1):69-73
Objectives: The aim of the following investigation was to quantify the resorption rate of tissue-engineered bone grafts in the maxillary sinus using volume measurements. MATERIAL AND METHODS: Sinus floor augmentation using autologous bone grafts from the iliac crest (n=17, group 1) was compared with commercially produced transplants of human cells seeded on polyglycolid-polylactid (PLGA) scaffolds (Oral Bone) (n=14, group 2). RESULTS: The total resorption rate for autologous transplants 3 months post operation was 29%, while the tissue-engineered bone showed a resorption rate of 90%. The autologous bone had a bone density of up to 266-551 Hounsfield units (HU), while sufficient mineralization of tissue-engineered bone was found in only one case (152 HU). CONCLUSION: In this clinical study, the use of autologous cancellous bone grafts in sinus augmentation was more reliable than scaffolds containing cultured osteoblasts. Further tissue-engineered bone transplants should be examined to draw general conclusions about the use of tissue-engineered grafts compared with autologous bone grafts for maxillary sinus augmentation. 相似文献