Spherical V-Fe-MCM-48: The Synthesis,Characterization and Hydrothermal Stability

Spherical MCM-48 mesoporous sieve co-doped with vanadium and iron was successfully synthesized via one-step hydrothermal method. The material was characterized by X-ray diffraction (XRD), nitrogen adsorption-desorption isotherms, inductively coupled plasma (ICP), scanning electron microscopy (SEM), transmission electron microscopy (TEM), diffuse reflectance UV-vis spectra, and X-ray photoelectron spectra (XPS) techniques. Results indicated that the V-Fe-MCM-48 showed an ordered 3D cubic mesostructure with spherical morphology, narrow pore size distribution and high specific surface area. Most of vanadium and iron atoms existing as tetrahedral V⁴⁺ and Fe³⁺ species were co-doped into the silicate framework. The particle sizes of V-Fe-MCM-48 were smaller and the specific area was much higher than those of of V-MCM-48. Additionally, the synthesized V-Fe-MCM-48 exhibited improved hydrothermal stability compared with the pure MCM-48.     

Syngeneic B16-F1 cells are more efficient than allogeneic Cloudman cells as antigen source in DC-based vaccination in the B16-F1 murine melanoma model

A major obstacle to obtaining relevant results in cancer vaccination has been the lack of identification of immunogenic antigens. Dendritic cell (DC)-based cancer vaccines used preventively may afford protection against tumor inoculation, but the effect of antigen choice on anti-tumor protection is not clear. When using irradiated syngeneic tumor cells to load DCs, tumor self-antigens are provided, including tumor-associated antigens (TAAs) and neoantigens generated by tumor mutations. On the other hand, allogeneic tumor cells could only supply shared TAAs. To assess the advantages of each source in protective vaccination, we analyzed in C57BL/6 mice the effect of loading DCs with irradiated syngeneic B16-F1 or allogeneic Cloudman melanoma cells; both cell lines were characterized by whole exome sequencing and RNAseq. Tumor cell components from the two irradiated cell lines were efficiently internalized by DCs, and transported to MHC-class II positive tubulovesicular compartments (MIICs). DCs loaded with allogeneic irradiated Cloudman cells (DC-ApoNec_ALLO) induced a partially effective anti-melanoma protection, although Cloudman and B16-F1 cells share the expression of melanocyte differentiation antigens (MDAs), cancer-testis antigens (CTAs) and other TAAs. DCs loaded with syngeneic B16-F1 cells (DC-ApoNec_SYN) established a more potent and long-lasting protection and induced a humoral anti-B16F1 response, thus suggesting that neoepitopes are needed for inducing long-lasting protection.     

Corrigendum to: “Use of dynamically adaptive grid techniques for the solution of electrochemical kinetic equations. Part 13. Patch-adaptive simulation of wave propagation along ring electrodes: one-dimensional approximation” [J. Electroanal. Chem. 529 (2002) 51–58]

A new electroactive polynuclear inorganic compound of a rare earth metal hexacyanoferrate, samarium hexacyanoferrate (SmHCF), was prepared by electrochemical deposition on the surface of a glassy carbon electrode with a potential cycling procedure. The cyclic voltammogram of SmHCF exhibits a pair of well-defined redox peaks with a formal potential of 191 mV (vs. SCE) at a scan rate of 100 mV/s in 0.2 M NaCl solution and the redox peak currents increase linearly with the scan rate up to 100 mV/s. The effects of the concentration of supporting electrolyte on the electrochemical characteristics of SmHCF and the transport behavior of K⁺, Na⁺ and Li⁺ counter ions through the ion channels of SmHCF were studied by voltammetry. The different electrochemical behavior of SmHCF in various cation-containing supporting electrolytes was investigated by cyclic voltammetry and scanning electron microscopy. The SmHCF was also characterized by FTIR techniques.     

人工全膝关节置换术后膝关节屈曲位对术后失血及膝关节活动度影响的研究进展

目的总结人工全膝关节置换术（total knee arthroplasty，TKA）后保持膝关节屈曲位对术后失血及早期膝关节活动度影响的研究进展。方法查阅近年国内外有关文献，就 TKA 术后保持膝关节屈曲位减少失血及增加膝关节活动度的作用机制，膝关节屈曲及伸直位、不同屈曲角度及维持时间的疗效差异进行总结。结果TKA 术后膝关节保持屈曲位可通过血管成角变化等减少术后失血、提高屈肌肌力，进而增加膝关节活动度。膝关节屈曲角度较大、维持屈曲位时间较长时，可显著改善术后失血和膝关节活动度；但维持时间≥24 h 与<24 h 相比，不能进一步减少失血并改善膝关节活动度。结论TKA 术后保持膝关节屈曲位是减少术后失血，提高早期膝关节活动度的简单、有效方法，但膝关节最佳屈曲角度及维持时间有待进一步探究。