排序方式: 共有4条查询结果,搜索用时 15 毫秒
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Thomas R. Porter Sharon L. Mulvagh Sahar S. Abdelmoneim Harald Becher J. Todd Belcik Michelle Bierig Jonathan Choy Nicola Gaibazzi Linda D. Gillam Rajesh Janardhanan Shelby Kutty Howard Leong-Poi Jonathan R. Lindner Michael L. Main Wilson Mathias Margaret M. Park Roxy Senior Flordeliza Villanueva 《Journal of the American Society of Echocardiography》2018,31(3):241-274
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Min Bai Ming Shen Yanwei Teng Ying Sun Fan Li Xiangyu Zhang Yuanyuan Xu Yourong Duan Lianfang Du 《Oncotarget》2015,6(41):43779-43790
The overexpression of the breast cancer resistance protein (ABCG2) confers resistance to Adriamycin (ADR) in breast cancer. The silencing of ABCG2 using small interfering RNA (siRNA) could be a promising approach to overcome multidrug resistance (MDR) in cancer cells. To deliver ABCG2-siRNA effectively into breast cancer cells, we used mPEG-PLGA-PLL (PEAL) nanoparticles (NPs) with ultrasound-targeted microbubble destruction (UTMD). PEAL NPs were prepared with an emulsion-solvent evaporation method. The NPs size was about 131.5 ± 6.5 nm. The siRNA stability in serum was enhanced. The intracellular ADR concentration increased after the introduction of siRNA-loaded NPs. After intravenous injection of PEAL NPs in tumor-bearing mice, the ABCG2-siRNA-loaded NPs with UTMD efficiently silenced the ABCG2 gene and enhanced the ADR susceptibility of MCF-7/ADR (ADR resistant human breast cancer cells). The siRNA-loaded NPs with UTMD + ADR showed better tumor inhibition effect and good safety in vivo. These results indicate that ADR-chemotherapy in combination with ABCG2-siRNA is an attractive strategy to treat breast cancer. 相似文献
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目的探讨超声靶向微泡破碎(UTMD)联合半乳糖聚乙烯亚胺(PEI-Gal)介导凋亡素基因对小鼠肝癌移植瘤的抑制作用。方法建立c57BL/6小鼠皮下肝癌移植瘤模型,随机分为4组:对照组;PEI-Gal+质粒组;PEI-Gal+超声+质粒组;PEI-Gal+UTMD+质粒组。对组织行冰冻切片采用免疫荧光法检测转染率、Tunel检测凋亡率。观察肿瘤的体积和组织学变化,同时计算抑瘤率。采用免疫组织化学法检测移植瘤Bcl-2及Caspase-3蛋白在各组肿瘤标本中的表达。结果PEI-Gal+UTMD+pEGFP-N3组基因转染效率明显高于对照组、PEI-Gal+质粒组与PEI-Gal+超声+质粒组(P均<0.01)且对组织无明显损伤。治疗12天后PEI-Gal+UTMD+pCDNA-VP3组肿瘤凋亡率(34.57%±3.56%)、抑瘤率(56.6%)均显著高于对照组和PEI-Gal+质粒组(P均<0.01)。肿瘤组织中Bcl-2蛋白表达明显下降,Caspase-3蛋白表达增加与其他各组比较差异均有统计学意义(P<0.01)。结论 超声靶向微泡破碎联合半乳糖聚乙烯亚胺能显著增强基因转染效率,且对组织无明显影响。联合凋亡素基因能通过诱导凋亡抑制肿瘤生长发挥抗瘤作用。 相似文献
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