臭氧联合治疗膝关节半月板损伤的临床研究

目的探讨臭氧冲洗联合手法整复治疗膝关节半月板损伤的临床效果。方法选择本院90例膝关节半月板损伤患者作为本次研究对象,病例收集时间为2017年5月-2018年5月。按照治疗分组,手法整复组采取手法整复治疗,臭氧治疗组采用臭氧冲洗治疗,手法整复联合臭氧组则采取手法整复治疗+臭氧冲洗治疗。比较三组疗效;膝关节疼痛缓解的时间、膝关节疼痛消失的时间;治疗前后患者膝关节疼痛VAS评分值以及生活质量QOL评分值;不良作用发生率。结果手法整复联合臭氧组疗效高于手法整复组与臭氧治疗组,P<0.05;手法整复联合臭氧组膝关节疼痛缓解的时间、膝关节疼痛消失的时间优于手法整复组与臭氧治疗组,P<0.05;治疗前三组膝关节疼痛VAS评分值以及生活质量QOL评分值接近,P>0.05;治疗后手法整复联合臭氧组膝关节疼痛VAS评分值以及生活质量QOL评分值优于手法整复组与臭氧治疗组,P<0.05。手法整复联合臭氧组不良作用发生率和手法整复组与臭氧治疗组接近,P>0.05。结论手法整复治疗+臭氧冲洗治疗膝关节半月板损伤的疗效好,可更好改善膝关节疼痛VAS评分值以及生活质量QOL评分值。     

微创臭氧治疗腰椎间盘突出症的临床疗效

目的评价微创臭氧治疗腰椎间盘突出症的临床疗效。方法选取2018年10月-2019年10月深圳市龙岗区第三人民医院收治的腰椎间盘突出症患者60例作为研究对象,按照抽签法分为对照组和观察组各30例。对照组给予传统治疗,观察组采用微创臭氧治疗,比较两组患者的视觉模拟评分量表(VAS)评分和日本骨科协会评分表(JOA)评分。结果两组VAS评分经治疗后均明显低于治疗前,且观察组治疗后的VAS评分明显低于对照组,差异均有统计学意义(P<0.05)。两组JOA评分经治疗后均明显高于治疗前,且观察组治疗后的JOA评分明显高于对照组,差异均有统计学意义(P<0.05)。结论微创臭氧治疗腰椎间盘突出症效果良好,不仅能缓解腰椎疼痛,还有助于腰椎功能的提升,值得临床推广应用。     

Efficacy of adjuvant ozone therapy in reducing postsurgical complications following impacted mandibular third-molar surgery: A systematic review and meta-analysis

BackgroundResults from several randomized controlled trials have shown a beneficial effect of ozone in reducing postsurgical complications after impacted mandibular third-molar surgery, but the literature is lacking a systematic review and meta-analysis.MethodsThe authors conducted this systematic review according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines after exclusion and inclusion criteria were applied and the following outcome parameters were evaluated: pain, swelling, trismus, quality of life, number of analgesics consumed, and adverse events. RevMan Cochrane Collaboration software, Version 5.3, was used to perform meta-analysis and the Grading of Recommendation Assessment, Development and Evaluation approach was used to rate the certainty of evidence.ResultsPatients who underwent adjuvant ozone application reported lower pain scores than patients in the control group at 24 hours after surgery (95% CI, –3.94 to –1.56) and at 7 days (95% CI, –1.67 to –0.78). Pooled analysis of all 4 included trials revealed a standardized mean difference (SMD) in swelling of –0.44 at 24 hours, 0.63 at 72 hours, and –0.87 at 7 days after surgery in the experimental group. Higher mean estimates in mouth opening were experienced by patients who received ozone at 24 hours (SMD, 2.74; 95% CI, –1.93 to 7.41; 4 studies, 133 patients), 72 hours (SMD, 2.77; 95% CI, –0.63 to 6.17; 4 studies, 133 patients), and 7 days after surgery (SMD, 1.42 SMD; 95% CI, –1.34 to 4.18; 4 studies, 133 patients).Practical ImplicationsEvidence suggests that adjuvant ozone application can offer some benefit for reducing pain, improving quality of life, and decreasing mean intake of analgesics after impacted mandibular third-molar surgery, but it is not effective in reducing facial swelling and trismus, which paves the way for future research.     

Acute inhalation of ozone induces DNA methylation of apelin in lungs of Long-Evans rats

Apelin has cardiopulmonary protective properties that promote vasodilation and maintenance of the endothelial barrier. While reductions in apelin have been identified as a contributor to various lung diseases, including pulmonary edema, its role in the effect of air pollutants has not been examined. Thus, in the current study, we sought to investigate if apelin is a downstream target of inhaled ozone and if such change in expression is related to altered DNA methylation in the lung. Male, Long-Evans rats were exposed to filtered air or 1.0?ppm ozone for 4?h. Ventilation changes were assessed using whole-body plethysmography immediately following exposure, and markers of pulmonary edema and inflammation were assessed in the bronchoaveolar lavage (BAL) fluid. The enzymatic regulators of DNA methylation were measured in the lung, along with methylation and hydroxymethylation of the apelin promoter. Data showed that ozone exposure was associated with increased enhanced pause and protein leakage in the BAL fluid. Ozone exposure reduced DNA cytosine-5-methyltransferase (DNMT) activity and Dnmt3a/b gene expression. Exposure-induced upregulation of proliferating cell nuclear antigen, indicative of DNA damage, repair, and maintenance methylation. Increased methylation and reduced hydroxymethylation were measured on the apelin promoter. These epigenetic modifications accompanied ozone-induced reduction of apelin expression and development of pulmonary edema. In conclusion, epigenetic regulation, specifically increased methylation of the apelin promoter downstream of DNA damage, may lead to reductions in protective signaling of the apelinergic system, contributing to the pulmonary edema observed following the exposure to oxidant air pollution.     

臭氧老化作用对SY-28硅橡胶机械性能的影响

目的：评估臭氧老化作用对SY-28硅橡胶机械性能的影响。方法：按照国家标准，对SY-28和MDx-4-4210硅橡胶臭氧老化前后的扯断强度、扯断伸长率、永久变形率（3min后）、撕裂强度、硬度（邵氏A）进行测试，并对其老化性能加以评价。结果：经臭氧老化作用处理，SY-28硅橡胶的永久变形率和邵氏硬度优于MDX-4-4210硅橡胶：扯断强度、扯断伸长率和撕裂强度与MDX-4-4210硅橡胶相似。结论：SY-28硅橡胶机械性能优良，同时具有良好的耐臭氧老化性能。     

The inability of Streptococcus mutans and Lactobacillus acidophilus to form a biofilm in vitro on dentine pretreated with ozone

Background: The use of ozone therapy in the treatment of dental caries is equivocal. The aim of this study was to use an in vitro model to determine the effects of prior ozone application to dentine on biofilm formation and to measure any associated reduction in bacteria viability. Methods: Twenty dentine discs were bonded to the bases of 5 mL polycarbonate screw top vials. Ten dentine discs were infused with ozone for 40 seconds, 10 samples remained untreated as a control. The vials were filled with nutrient medium, sterilized and placed into the outflow from a continuous chemostat culture of Streptococcus mutans and Lactobacillus acidophilus for four weeks. At the conclusion of the experiment bacterial growth was monitored by taking optical density readings of the growth medium in each vial and the outer surface of the dentine specimens were examined by scanning electron microscopy as shown by SEM analysis. Results: Ozone infusion prevented biofilm formation on all the treated samples while there was substantial biofilm present on the control specimens. While the average optical density of the control specimens was almost twice that of the ozone infused dentine (0.710 for the control with a SD of 0.288 and 0.446 for the ozonated samples with a SD of 0.371), the results were not significant (p > 0.05). Conclusions: This preliminary study has shown that the infusion of ozone into non‐carious dentine prevented biofilm formation in vitro from S. mutans and L. acidophilus over a four‐week period. The possibility exists that ozone treatment may alter the surface wettability of dentine through reaction with organic constituents.     

低温等离子射频消融联合臭氧消融术治疗颈椎间盘突出症的临床研究

目的 探讨低温等离子射频消融联合臭氧消融治疗颈椎间盘突出症的临床效果以及对炎症反应的影响。方法 回顾性分析2018年3月-2021年3月我科收治的65例单节段颈椎间盘突出症手术患者的基本资料及相关数据。按照所采取手术方式将其分为复合组(36例)和单纯组(29例), 复合组患者采用低温等离子射频消融术联合臭氧治疗,单纯组患者仅采用低温等离子射频消融术治疗。分别采集术前、术后3天与术后1个月随访时的视觉模拟评分（visual analogue scale, VAS）,记录术前及术后72小时最常见伴随症状的变化以及抽取的静脉血中血沉、C反应蛋白和白细胞计数,对两组结果进行统计比较;并对两组的不良反应发生率进行比较。结果 两组患者术后3天及术后1个月疼痛评分较术前均显著降低(P<0.05),但两组间比较仅有术后第三天差异具有统计学意义(P＜0.05);复合组患者术后血沉、C反应蛋白及白细胞计数均低于单纯组(P<0.05);两组的不良反应发生率无统计学差异（P＞0.05）。结论 低温等离子射频消融联合臭氧消融术可有效缓解颈椎间盘突出症患者的疼痛症状,并能更好预防术后炎症的发生。     

菱形阻滞联合腔内臭氧注射和运动康复治疗膝关节骨性关节炎的疗效分析

目的 分析菱形阻滞联合腔内臭氧注射和运动康复治疗膝关节骨性关节炎的疗效。方法 选取 2018年1月—2018年7月于四川省骨科医院收治的膝关节骨性关节炎患者100例作为研究对象。根据随机数字表 法将患者分为对照组和观察组,各50例。对照组患者给予腔内臭氧注射和运动康复治疗,观察组患者在此基础 上采用菱形阻滞治疗。分别于治疗前及治疗6周后用数字评价量表（NRS）评价患者疼痛程度,西安大略省和 麦克马斯特大学骨关节炎指数（WOMAC）评价患者骨关节炎严重程度。分别于治疗前及治疗12个月后,利 用奎森功能演算指数（Lequesne）和国际膝部文件委员会（IKDC）膝部功能主观评估表评价患者远期疗效。 结果 观察组治疗后NRS评分、WOMAC评分较对照组低（P <0.05）。两组治疗后NRS评分、WOMAC评分 较治疗前低 （P <0.05）。观察组治疗后IKDC膝部功能主观评估表评分较对照组高,Lequesne评分较对照组 低（P <0.05）。两组治疗后IKDC膝部功能主观评估表评分较治疗前高,Lequesne评分较治疗前低（P <0.05）。 结论 菱形阻滞联合腔内臭氧注射和运动康复治疗膝关节骨性关节炎疗效显著,可在近期减轻患者疼痛,降低 患者骨关节炎严重程度,且在远期改善患者膝关节功能方面也具有一定的优势。     

试论医用臭氧治疗腰椎间盘突出症的中医学理论基础

詹红生教授认为“气虚痰瘀、络脉痹阻”是腰椎间盘突出的主要病机,治疗上应以补气为基本原则,并兼顾化痰散瘀。临床上,经皮椎间盘医用臭氧注射技术正是基于“补气理论”,通过局部“补气”以发挥气的推动和温煦作用,从而达到改善血运、促进炎性水肿和突出物吸收的目的。     

臭氧水对水泡性口炎病毒灭活效果的检测

经检测，在２０～２６℃室温下，浓度为５０±１ｍｇ／Ｌ的臭氧水溶液，作用３０ｓ，可灭活悬液中水泡性口炎病毒；用其冲洗、作用１ｍｉｎ，可灭活干燥于玻璃片上的水泡性口炎病毒。