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目的 探讨雌激素药物治疗对慢性间歇性低氧(chronic intermittent hypoxia,CIH)大鼠颏舌肌肌型磷酸果糖激酶(phosphofructokinase muscle-specific isoform,PFK-M)表达的影响,以期从能量代谢的角度探讨雌激素用于治疗阻塞性睡眠呼吸暂停低通气综合征(OSAHS)的可能机制.方法 选择2个月龄健康雄性SD大鼠50只,用随机排列表分为5组:正常对照组(NC)、慢性间歇性低氧组(CIH)、低剂量雌激素治疗组(LE)、中剂量雌激素治疗组(ME)、高剂量雌激素治疗组(HE),每组10只.后4组建立CIH模型(8 h/d,5周);后3组分别给予0.1、0.2、0.3 mg/kg的苯甲酸雌二醇肌肉注射,NC、CIH组给予无菌橄榄油肌肉注射.实时反转录聚合酶链反应(real-time RT-PCR)检测大鼠颏舌肌PFK-M mRNA表达;蛋白质印迹法分析PFK-M蛋白表达.结果 CIH组PFK-M mRNA和蛋白的表达量分别为(2.144±0.260)和(0.875±0.025),与NC组相比(1.000±0.259,0.413±0.013)均显著升高(P<0.05).LE、ME、HE组PFK-M mRNA的表达量分别为(1.424±0.193)、(1.395±0.251)和(1.310±0.094),PFK-M蛋白的表达量分别为(0.638±0.015)、(0.576±0.017)和(0.505±0.021),与CIH组相比,3个雌激素治疗组的PFK-M mRNA和蛋白表达均显著降低(P<0.05).3个雌激素治疗组间两两比较显示,HE组PFK-M蛋白表达量比LE组明显降低(P<0.05),而PFK-M mRNA表达量的差异无统计学意义(P>0.05).结论 CIH可导致大鼠颏舌肌内PFK-M转录和翻译水平的升高,而雌激素药物治疗可显著抑制PFK-M的过度表达,并呈现一定的剂量依赖趋势.  相似文献   
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目的 探讨开胸手术患者红细胞内糖代谢限速酶活性的变化及不同麻醉方法对其的影响。方法 48例ASAI~Ⅱ级择期开胸手术患者,按麻醉方式随机分成三组,每组16例。Ⅰ组采用地氟醚吸入为主的全身麻醉;Ⅱ组采用异氟醚吸入为主的全身麻醉;Ⅲ组采用异氟醚吸入联合连续硬膜外阻滞。于麻醉前、手术90min、术后60min及术后第1、2天共五个时点分别测定血糖浓度及红细胞6-磷酸葡萄糖脱氢酶(G-6PD)、磷酸果糖激酶(PFK)和醛糖还原酶(AR)活性。结果 与麻醉前比较,三组患者血糖浓度自术中90min开始,至术后第2天升高显著(P<0.05);Ⅰ、Ⅱ两组术后第1天PFK活性显著下降(P<0.05),G-6PD、AR活性显著升高(P<0.05),而Ⅲ组各时点红细胞内糖代谢限速酶活性的变化与麻醉前比较均无统计学差异(P>0.05),且Ⅲ组术后第1天PFK值远低于Ⅰ组相应值。结论 开胸手术中、手术后存在明显的高血糖反应。术后第1天,红细胞内会出现糖酵解途径受抑制,磷酸戊糖途径、多元醇通路相应活跃现象。采用异氟醚吸入联合硬膜外阻滞可在一定程度上调控手术创伤对红细胞糖代谢的影响。  相似文献   
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Objective

To investigate the effect of Ruyanneixiao cream (RYNX) on the expression of hypoxia inducible factor-1α (HIF-1α), hexokinase 2 (HK2), phosphofructokinase (PFK), and pyruvate kinase M2 (PKM2) mRNA and protein in MCF-10AT cells and in an animal model of precancerous mammary lesions.

Methods

Following treatment of MCF-10AT cells with RYNX, tamoxifen (TAM) and YC-1 for 48 h, HIF-1α, HK2, PFK, PKM2 mRNA and protein expression was analyzed. Fifty female SD rats were randomly divided into control, model, TAM, and high- and low-dose RYNX groups, with 10 rats in each group. A precancerous mammary lesion model was established for all groups except the control group. High- and low-dose RYNX cream containing TAM was coated on the breasts of animals in the corresponding groups. The rat mammary tissue was removed in the 10th week and HIF-1α, HK2, PFK, PKM2 mRNA and protein was analyzed.

Results

In vitro analyses demonstrated that, compared with the matrix group, HIF-1α, HK2, PFK, PKM2 mRNA and protein expression was significantly decreased in the RYNX group (P < 0.05). Compared with the YC-1 + RYNX group, HK2, PFK, and PKM2 protein expression was significantly reduced in the RYNX group. HIF-1α, HK2, PFK, and PKM2 protein expression was increased significantly in the model group (P < 0.05) compared with the control group, while HIF-1α, HK2, PFK, and PKM2 mRNA and protein expression was significantly decreased in both the high- and low-dose RYNX groups (P < 0.05), with the effect being greater in the high-dose group.

Conclusion

RYNX can block precancerous breast lesions by decreasing the expression of HK2, PFK, and PKM2 mRNA and protein via inhibition of HIF-1α mRNA and protein overexpression in a dose-dependent manner.  相似文献   
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目的 观察兔脑出血后血肿周围组织能量代谢产物的变化规律。方法 健康大耳白家兔采用随机数字法分成 3组 :正常组 ,脑出血组 ,假手术组。其中脑出血、假手术组又分成 1、6、12、2 4、4 8、72h组。采用二次注血法制备兔自体血脑出血模型。分离血肿周围组织 ,测定脑水含量、能量代谢产物三磷酸腺苷 (ATP)和乳酸的含量以及磷酸果糖激酶活性变化。结果 脑出血 1h血肿侧即有脑水肿 ,至 72h逐渐加重 ,血肿侧明显重于对侧。 1h后血肿周围组织ATP含量呈中度下降 ,但差异无显著意义。 1~ 12h间ATP含量略有波动 ,12h后再次下降 ,至 2 4h达最低点 [(13 2 9± 2 92 )μg/ g ,降至正常的 5 8% ],差异有显著意义。 2 4h至 72h间无明显变化。乳酸含量于 1h即有增高 ,12h达高峰 [(2 1 0 1± 0 18) μmol/g],直至 72h乳酸水平 [(12 89± 0 2 5 ) μmol/g]仍明显高于正常组及假手术组。磷酸果糖激酶 (PFK)活性 1h即有下降 [(3 98± 0 0 2 )U/mg],72h内持续下降。与正常组及假手术组比较差异有显著意义。结论 脑出血后血肿周围组织在 2 4h后才发生明显的能量代谢衰竭 ,滞后于脑水肿的发生 ,其发生机制可能与相关酶的活性减低有关  相似文献   
5.
Objective: To compare the effects of different methods of anesthesia and analgesia on the activities of phosphofructokinase(PFK), glucose-6-phosphate dehydrogenase(G-6PD) and aldose reductase(AR) in erythrocytes and levels of plasma glucose and stress hormones in patients undergoing esophagus surgery. Methods: Sixty-two patients scheduled for esophagus surgery were randomly divided into three groups: groupⅠ(n = 20) receiving only general anesthesia(GA) followed by intravenous patient controlled analgesia(PCA) with fentanyl 15μg/kg. The other two groups receiving both general anesthesia combined with thoracic epidural anesthesia (GEA) and either intravenous PCA with fentanyl 15μg/kg (groupⅡ, n = 21) or thoracic epidural analgesia(TEA) with 0.125% ropivacaine and 0.0002% fentanyl mixture (groupⅢ, n = 21) after the operation. Venous blood samples were collected for measurements of PFK, G-6PD and AR activities in erythrocytes and plasma glucose, cortisol, epinephrine and norepinephrine before induction (T1), 60 min following the incision (T2), 60 min(T3) after operation, on the 1st(T4) and 2nd postoperative day(T5). Results: The activities of PFK decreased(P < 0.01 or P = 0. 004) and the activities of G-6PD and AR increased(P < 0. 01) in groupsⅠand Ⅱ on T4 compared with those on T1. Between the two groups, the activities of these enzymes in groupⅡchanged less than those of groupⅠ(P < 0.01 or P < 0.05). These enzymes activities changed slightly in group Ⅲ on T4(P > 0.05). There were significant differences between group Ⅲ and the other two groups(P < 0.01 or P < 0. 05). The levels of plasma glucose increased significantly on T2(P < 0.01), reached peak values on T4(P < 0.01) and fell on T5 in the three groups. Compared to those of groupsⅠandⅡ, the values of plasma glucose in group Ⅲ were lower on T4 and T5(P < 0.05 or P < 0.01). The cortisol concentration in each group increased significantly at T2(P < 0.01 or P < 0.05), and remained elevated on T5(P < 0.01 or P < 0.05), while on T2 and T3 the cortisol levels of groupⅠwere higher than that of groupsⅡand Ⅲ(P < 0.05). The levels of group Ⅲwere lower than those of the other groups on T4 and T5(P < 0.01 or P < 0.05). The levels of epinephrine and norepinephrine were also significantly higher in group I than those of the other two groups on T2(P < 0.01 or P < 0.05), and their levels in group ⅠandⅡwere higher than that of group Ⅲ on T4. The patients of the three groups obtained satisfactory pain relief, with all Vidual Analogue Scale(VAS) scores less than 3. VAS scores of groupⅠwere much greater 4h after operation. Group Ⅲ VAS scores were the lowest 24h after operation. However, the number of times patients pressed the bolus switch was higher in groupⅡ(P < 0.01). Conclusion: Compared with GA and intravenous PCA, general anesthesia combined with thoracic epidural anesthesia and analgesia obtain better pain relief and could markedly alleviate the stress response and improve these erythrocyte glucose metabolism changes after esophagus surgery.  相似文献   
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