全文获取类型
收费全文 | 856篇 |
免费 | 38篇 |
国内免费 | 15篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 13篇 |
妇产科学 | 9篇 |
基础医学 | 135篇 |
口腔科学 | 6篇 |
临床医学 | 108篇 |
内科学 | 107篇 |
皮肤病学 | 10篇 |
神经病学 | 47篇 |
特种医学 | 77篇 |
外国民族医学 | 2篇 |
外科学 | 31篇 |
综合类 | 110篇 |
预防医学 | 82篇 |
眼科学 | 14篇 |
药学 | 116篇 |
中国医学 | 27篇 |
肿瘤学 | 12篇 |
出版年
2023年 | 8篇 |
2022年 | 8篇 |
2021年 | 19篇 |
2020年 | 28篇 |
2019年 | 19篇 |
2018年 | 25篇 |
2017年 | 31篇 |
2016年 | 17篇 |
2015年 | 30篇 |
2014年 | 72篇 |
2013年 | 43篇 |
2012年 | 40篇 |
2011年 | 56篇 |
2010年 | 36篇 |
2009年 | 39篇 |
2008年 | 60篇 |
2007年 | 42篇 |
2006年 | 27篇 |
2005年 | 32篇 |
2004年 | 25篇 |
2003年 | 25篇 |
2002年 | 20篇 |
2001年 | 19篇 |
2000年 | 11篇 |
1999年 | 11篇 |
1998年 | 8篇 |
1997年 | 8篇 |
1996年 | 9篇 |
1995年 | 5篇 |
1994年 | 8篇 |
1993年 | 11篇 |
1992年 | 4篇 |
1991年 | 7篇 |
1990年 | 6篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 13篇 |
1984年 | 12篇 |
1983年 | 9篇 |
1982年 | 10篇 |
1981年 | 18篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1972年 | 1篇 |
1969年 | 1篇 |
排序方式: 共有909条查询结果,搜索用时 15 毫秒
1.
目的对当归不同炮制品中大肠埃希菌变化进行定量分析。方法建立荧光定量PCR方法,对当归不同炮制品、不同产地、不同收集企业、不同储藏时间的大肠埃希菌进行定量分析。结果不同炮制品中大肠埃希菌数量变化为生当归土炒当归酒当归;在不同产地的当归中以甘肃岷县地区所含的大肠埃希菌的数量最少;与零售企业相比,生产销售企业的当归和酒当归中大肠埃希菌的数量较少;不同储藏时间对当归和酒当归中大肠埃希菌数量有一定影响,随储藏时间的增加,大肠埃希菌的数量也在增加。对部分代表性样品进行平板计数法与荧光定量PCR法比较时发现,平板计数法结果多呈阴性,荧光定量PCR结果均呈阳性。结论所建立的荧光定量PCR法特异性、灵敏度、可靠性以及报告周期均优于平板计数法,可为当归不同炮制品中大肠埃希菌的快速、准确定量检测提供有效手段。 相似文献
2.
The metastasis of cervical cancer has always been a clinical challenge. We investigated the effects of low-dose naltrexone (LDN) on the epithelial mesenchymal transition of cervical cancer cells in vitro as well as its influence on macrophage polarization and associated cytokines in vivo. The results suggested that LDN supressed the proliferation, migration and invasion abilities and promote their apoptosis in Hela cells, whereas the opioid growth factor receptor (OGFr) silenced significantly reversed these effects in vitro. Knockdown the expression of OGFr, the inhibitory of LDN on EMT was weakened. LDN could inhibit cervical cancer progression in nude mice. In additon, LDN indirectly reduced the number of tumor-associated macrophages (TAMs), mainly M2 macrophages, and decreased expression of anti-inflammatory factor IL-10 in the serum of nude mice. These findings demonstrate that LDN could be a potential treatment for cervical cancer. 相似文献
3.
Notch1 has been implicated in asthma pathogenesis. However, the function of Notch1 in regulating airway smooth muscle (ASM) cell proliferation and migration during airway remodeling of asthma remains unknown. Using an in vitro model induced by tumor necrosis factor (TNF)-α, we reported in this study that Notch1 participated in TNF-α-induced proliferation and migration of ASM cells. Our results demonstrated that Notch1 expression was significantly upregulated in ASM cells exposed to TNF-α. Notch1 inhibition significantly repressed TNF-α-induced ASM cell proliferation and migration, while Notch1 overexpression promoted the opposite effect. Moreover, Notch1 inhibition downregulated the expression of Notch-1 intracellular domain (NICD) and Hes1, while upregulated PTEN expression in TNF-α-exposed cells. Notably, Hes1 overexpression partially reversed the Notch1-inhibition-mediated inhibitory effect on TNF-α-induced ASM cell proliferation and migration. In addition, the promoting effect of Notch1 inhibition on PTEN expression was markedly abrogated by Hes1 overexpression. Overall, these findings demonstrated that Notch1 inhibition repressed TNF-α-induced ASM cell proliferation and migration by modulating the Hes1/PTEN signaling axis, a finding that highlights the involvement of Notch1/Hes1/PTEN in regulating airway remodeling of asthma. 相似文献
4.
5.
6.
《药学学报(英文版)》2020,10(7):1294-1308
A great challenge in multi-targeting drug discovery is to identify drug-like lead compounds with therapeutic advantages over single target inhibitors and drug combinations. Inspired by our previous efforts in designing antitumor evodiamine derivatives, herein selective histone deacetylase 1 (HDAC1) and topoisomerase 2 (TOP2) dual inhibitors were successfully identified, which showed potent in vitro and in vivo antitumor potency. Particularly, compound 30a was orally active and possessed excellent in vivo antitumor activity in the HCT116 xenograft model (TGI = 75.2%, 150 mg/kg, p.o.) without significant toxicity, which was more potent than HDAC inhibitor vorinostat, TOP inhibitor evodiamine and their combination. Taken together, this study highlights the therapeutic advantages of evodiamine-based HDAC1/TOP2 dual inhibitors and provides valuable leads for the development of novel multi-targeting antitumor agents. 相似文献
7.
Bowen Qi Ayrianne J. Crawford Nicholas E. Wojtynek Megan B. Holmes Joshua J. Souchek Graca Almeida-Porada Quan P. Ly Samuel M. Cohen Michael A. Hollingsworth Aaron M. Mohs 《Nanomedicine : nanotechnology, biology, and medicine》2018,14(3):769-780
Pancreatic ductal adenocarcinoma is highly lethal and surgical resection is the only potential curative treatment for the disease. In this study, hyaluronic acid derived nanoparticles with physico-chemically entrapped indocyanine green, termed NanoICG, were utilized for intraoperative near infrared fluorescence detection of pancreatic cancer. NanoICG was not cytotoxic to healthy pancreatic epithelial cells and did not induce chemotaxis or phagocytosis, it accumulated significantly within the pancreas in an orthotopic pancreatic ductal adenocarcinoma model, and demonstrated contrast-enhancement for pancreatic lesions relative to non-diseased portions of the pancreas. Fluorescence microscopy showed higher fluorescence intensity in pancreatic lesions and splenic metastases due to NanoICG compared to ICG alone. The in vivo safety profile of NanoICG, including, biochemical, hematological, and pathological analysis of NanoICG-treated healthy mice, indicates negligible toxicity. These results suggest that NanoICG is a promising contrast agent for intraoperative detection of pancreatic tumors. 相似文献
8.
目的:观察安坤种子丸对卵巢储备功能下降致不孕症患者卵巢窦卵泡数目(AFC)和卵巢体积(OV)的影响。方法:卵巢储备功能下降致不孕患者32例,于月经干净后第3日口服安坤种子丸,10粒/次,3次/d,经前1周及经期、急性炎症期停药(闭经者除外);服用3个月经周期为1个疗程,连续治疗6月,采用自身对照法观察治疗后AFC和OV变化情况,血清卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)及月经、妊娠及中医症状积分等改善情况。结果:中医症状积分较治疗前明显下降,E2水平明显上升,FSH水平明显下降,差异有统计学意义(P0.05);LH无明显变化,差异无统计学意义(P0.05)。基础窦卵泡数目较治疗前增加(P0.05),差异有统计学意义(P0.05);卵巢体积(纵径、横径、前后径)较治疗前增大,差异有统计学意义(PO.05)。治疗后6月10例患者妊娠,且月经周期、经量恢复正常,2例患者月经及其兼证等无明显改善。结论:安坤种子丸能改善卵巢储备功能下降致不孕患者的卵巢功能,减轻临床症状,可能与促进AFC和OV正常反应有关。 相似文献
9.
5DF血球分析仪检测白细胞分类计数与瑞氏染色镜检结果对比研究 总被引:3,自引:2,他引:3
目的 探讨5DF血球分析仪白细胞分类计数与传统的瑞氏染色镜检计数结果之间的差异。方法 用5分类血球分析仪和瑞氏染色法同时对10 0份血样进行分类计数,并用统计学分析研究。结果 中性粒细胞、嗜酸、嗜碱性细胞和淋巴细胞分类计数两法无明显差异(P均>0 .0 5 ) ,但单核细胞分类计数仪器法比镜检法结果偏高(P <0 .0 0 1)。结论 对于一般血液常规检查可选用仪器法筛查,而血液系统疾病或某种原因引起的细胞结构改变应选用镜检法。 相似文献
10.
Yvonne P.J. Bosch Raed Al DieriHugo ten Cate Patty J. NelemansSaartje Bloemen Bas de LaatCoenraad Hemker Patrick W. WeerwindJos G. Maessen Baheramsjah Mochtar 《Thrombosis research》2014