石蒜碱诱导肺癌NCI-H460细胞凋亡作用及其机制

目的 通过研究石蒜碱在体外对人肺癌NCI-H460细胞增殖抑制和诱导凋亡的作用，探讨石蒜碱抗肿瘤机制。方法 MTT法检测石蒜碱对NCI-H460细胞增殖的抑制作用，流式细胞术检测NCI-H460细胞的凋亡率，比色法检测凋亡因子相关因子Caspase 3活性。结果 石蒜碱能够有效抑制肺癌NCI-H460细胞增殖，IC₅₀为（5.79±0.11）μmol/L，对NCI-H460细胞的凋亡具有诱导效应，可增强肺癌Caspase 3的活性。结论 石蒜碱在体外能有效地抑制NCI-H460细胞增殖并促进其凋亡，其机制可能与激活Caspase 3的表达从而诱导肿瘤细胞凋亡有关。     

Renal Kallikrein-Kinin System and the Depressor Effect of Angiotensin Converting Enzyme Inhibitors MK 421, SA 446, And Captopril In Rats

Responses in urinary kallikrein and kinin excretion and systolic blood pressure to MK 421, SA 446 or captopril were studied in normotensive rats fed on a regular or a low sodium diet to assess the role of renal kallikrein-kinin system in their hypotensive effect. MK 421, SA 446 or captopril were infused at a rate of 6 mg/kg/day by osmotic minipump implanted intraperito-neally for up to 6 days. The magnitude of fall in systolic blood pressure was greater on a low sodium diet when compared to on a regular diet, whereas the pattern of the fall was similar on both diets. The magnitude of falls in plasma angiotensin II and aldosterone concentration induced by MK 421, SA 446 and captopril was not significantly different between both regular and low sodium diets. Urinary kallikrein and kinin excretion and sodium excretion were increased during infusion of MK 421, SA 446 or captopril on a low sodium diet, however any significant changes were not found in each of them on a regular diet

The present results suggest that on a low sodium diet the augmented hypotensive response to angiotensin converting enzyme inhibitors in the rats might be due to the enhanced renal kallikrein-kinin system in addition to suppressed renin-angiotensin system     

低剂量X射线照射对荷人小细胞肺癌(NCI-H446)裸小鼠移植瘤细胞凋亡相关基因mRNA表达的影响

目的 研究低剂量照射（LDR）对荷人小细胞肺癌（NCI-H446）裸小鼠移植瘤细胞凋亡相关基因mRNA表达的影响。方法 对荷人小细胞肺癌裸小鼠进行全身深部X射线照射，采用原位杂交技术检测荷人小细胞肺癌裸小鼠移植瘤组织的p53、Bcl-2、Bax的mRNA表达水平。结果 D1（75mGy）、D2（4Gy）照射后，小细胞肺癌细胞的p53、Bax的mRNA表达呈上升趋势，Bcl-2的mRNA表达呈下降趋势，但与假照组（0 mGy）比较，无明显差异；D1＋D2组的p53、Bax的mRNA表达明显增多，Bcl-2mRNA表达显著下降，与假照组（0mGy）比较有统计学差异（P〈0．05）。结论 LDR在一定程度上可能上调小细胞肺癌细胞的p53、Bax的mRNA表达，下调了Bcl-2mRNA的表达，同时对其后的大剂量照射有协同作用。     

丙戊酸联合顺铂对体外人非小细胞肺癌细胞的增殖抑制作用

目的探讨丙戊酸（VPA）联合顺铂（DDP）对人非小细胞肺癌细胞A549、NCI．H460增殖抑制及细胞凋亡的影响,以及两药联合影响肺癌细胞增殖的机制。方法3组不同质量浓度的VPA与DDP单药及联合作用于人非小细胞肺癌细胞A549、NCI—H460,培养24、48、72h后,观察细胞数量和形态的变化,用MTF法分析药物对细胞增殖的抑制率（IR）及联合用药对细胞增殖抑制率q值,Hoechst／PI双染检测细胞凋亡的数量变化,Westernblot观察联合用药对Bcl．2、Caspase．3、Caspase．8蛋白的表达变化。结果培养48h后,各用药组细胞数目减少十分明显,其中VPA＋DDP组较单独用药组减少更为显著,其细胞形态发生较大改变;MTF法及q值计算结果显示,对A549细胞,VPA的增殖抑制作用呈现明显的时间与浓度依赖性,在高质量浓度时（300mg·L-1）与DDP具有协同作用;对NCI—H460细胞,当VPA的质量浓度≤100mg·L-1时,未显示出明显的时间与浓度依赖性,所有浓度均未显示出VPA与DDP的协同作用。VPA联合DDP可以进一步促进A549与NCI—H460的细胞凋亡,导致两细胞中凋亡因子Bcl-2水平的明显下调。联合作用对A549细胞更为明显,可能与该细胞中抑凋亡因子Bcl-2低表达及促凋亡因子Caspase-3、Caspasel8高表达有关。结论VPA能增强DDP对人非小细胞肺癌细胞的增殖抑制作用,联合作用的协同性与药物浓度及细胞类型有关,不同类型细胞对药物敏感性的差异可能与细胞自身表达的凋亡因子的含量有关。     

不同IL-15基因转染对NCI-H446细胞诱导PBMC增殖和杀伤肿瘤细胞的影响

目的 研究不同IL-15基因转染对NCI-H446细胞诱导外周血单个核细胞(PBMC)增殖和杀伤肿瘤细胞的影响.方法 野生型NCI-H446细胞(Cw)和被3种IL-15基因分别转染的3种NCI-H446细胞(Cmp:被IL-15成熟肽基因转染;Cp:被原型IL-15基因转染;Csp:被信号肽换为IL-2信号肽的改型IL-15基因转染),用丝裂霉素(M)处理后(分别名为MCw、MCmp、MCp和MCsp)作为刺激细胞刺激健康志愿者的PBMC.对这些被刺激的PBMC,分别名为MCw-PBMC、MCmp-PBMC、MCp-PBMC和MC-sp-PBMC.台盼蓝拒染法计数细胞数,流式细胞术测定CD4 细胞和CD8 细胞百分率.在MCmp-PBMC,MCp-PBMC和MCsp-PBMC中,选择其细胞数和CD4 细胞和/或CD8 细胞百分率统计学上明显高于MCw-PBMC的作为效应细胞,MTT法测定它们对Cw的杀伤.结果 与MCw-PBMC相比,MCp-PBMC在细胞数、CD4 细胞和CD8 细胞百分率及对Cw的杀伤上,均显著提高(P＜0.05).结论 原型IL-15基因转染能提高NCI-H446细胞诱导PBMC增殖和杀伤野生型NCI-H446细胞的能力.     

HA14-1联合顺铂诱导人小细胞肺癌细胞凋亡的研究

目的:观察HA14-1及其联合顺铂对人小细胞肺癌NCI-H446细胞凋亡诱导作用并探讨其可能的作用机制,探讨其对Bcl-2、Bax表达的的影响。方法:选用人小细胞肺癌NCI-H446细胞株为研究对象,分为空白组、DDP组、HA14-1组及DDP+HA14-1组,分别作用24、48 h后,采用MTT法检测细胞抑制率,流式细胞仪检测细胞凋亡,Real time PCR法比较细胞内Bcl-2、Bax表达变化。结果:随着HA14-1及DDP浓度的增加,细胞抑制率逐渐增加,细胞凋亡率增高,联合用药组较单独用药组细胞的抑制率,凋亡率均增高,HA14-1作用细胞后,Bcl-2表达水平下降,Bax表达水平增高。结论:HA14-1可以诱导人小细胞肺癌凋亡,并增加肺癌细胞对DDP的化疗作用。     

Population study and evaluation of 20 Y-chromosome STR loci in Germans

The nine European minimal haplotype (EMH) loci, the two SWGDAM loci and five further single-copy Y-chromosomal short tandem repeats (Y-STRs) DYS446, DYS447, DYS448, DYS449, DYS463, and the multicopy loci DYS464 were evaluated in the German population groups Dresden, Hamburg, Rostock, Munich, and the Sorbs who are a Slavic-speaking minority in Lusatia. Highest gene diversities in all populations were shown for DYS464, DYS385, and DYS449 (D=0.8559–0.9486). The haplotype diversity for the European minimal haplotype loci ranged between 0.9852 for Sorbs and 0.9983 for the Hamburg population showing that there is a significant portion of haplotypes which could not be resolved. Advanced typing using DYS446, DYS447, DYS448, DYS449, DYS463, and DYS464 discriminated all non-related individuals of the Dresden, Hamburg, and Rostock populations. Evaluation of the Y-STRs was accomplished by sequence analysis of all allelic fragments of the allelic ladders and microvariant alleles of DYS385 and the determination of the amounts of stutter products of the loci. Electronic Supplementary Material Supplementary material is available for this article at