通塞益脑液治疗急性缺血性中风及其微循环作用

通过临床研究观察了通塞益脑液治疗急性缺血性中风的临床疗效及作用机理.结果表明,通塞益脑液与有效对照组药物低分子右旋糖酐加维脑路通片疗效相似,以及通塞益脑液具有改善微循环的作用.     

通塞益脑液治疗急性脑梗塞的机理探讨

本文以结扎左侧颈总动脉造成实验性急性脑梗塞的沙土鼠模型,探讨通塞益脑液治疗急性脑梗塞的机理。发现结扎术前胃饲通塞益脑液者,可降低急性脑梗塞后脑组织中过氧化脂质的含量;结扎术后给药者,可降低脑组织中钙的含量。初步认为通塞益脑液对急性脑梗塞的脑组织有保护作用。     

Ultracryotomy: development and application (with examples from the yeast cytology) (author's transl)

The preparation steps usually necessary for obtaining ultrathin frozen sections of biological material (chemical prefixation, enclosing, cryoprotective treatment, freezing, sectioning, and post-staining the sections for transmission electron microscopy) are submitted to a critical analysis. The application of cryo-ultramicrotomy, in particularly for cytochemical purposes, is reviewed. Fundamental considerations of chemical prefixation and poststaining are supported by examples from yeast cytology. Furthermore, the efficiency of the cryo-ultramicrotomy (electron optical resolution of ultrastructural details) is demonstrated on yeast cells and protoplasts.     

醒脑静治疗安定中毒的临床观察

目的:探讨醒脑静注射液联合纳络酮注射液在急性安定中毒治疗中的疗效。方法:本研究采用醒脑静注射液联合纳络酮注射液治疗急性安定中毒55例并与单纯纳络酮治疗的51例进行对照。106例患者均经静脉血毒物测定为安定中毒;来诊时均有意识障碍,平均年龄(30.35±7.95)合并酒精中毒及其他疾病20例,常规给予洗胃、补液、利尿促排泄、纠正电解质失衡、吸氧及抗感染等对症处理。结果:醒脑静注射液治疗组与对照组患者比较,神志恢复时间(h),治疗组(1.73±0.98)显著短于对照组(3.22±1.38)(P<0.01);同组患者就诊时与清醒后的神志、呼吸、血压、心率及瞳孔等临床指标比较差异有显著性(P<0.05)。结论:醒脑静注射液与纳络酮联合应用,治疗急性安定中毒与对照组比较起效快、疗效确定,临床应用安全可靠。     

~(60)Co辐照灭菌对NAO-PLA微球理化特性及体外释放的影响

以 DL-聚乳酸(PLA)为囊材,醋炔诺酮肟(NAO)为主药的微球,可望作为长效避孕注射剂。由于 PLA 的玻璃化温度(T_g)较低,该微球不能采用一般的加热灭菌方法。本文采用10和25kGy 的两种~(60)Co辐照剂量对微球灭菌。对灭菌效果、NAO 和 PLA 以及微球灭菌前后的理化特性和体外释药速率等的比较,表明辐照剂量以10kGy 为宜。     

脑瘤消胶囊对荷S180肉瘤小鼠的抗肿瘤作用

目的　观察脑瘤消胶囊对实验性荷S180肉瘤小鼠的抗肿瘤作用 ,探讨脑瘤消胶囊治疗颅内肿瘤的作用机制。方法　随机将小鼠分为 6组 :正常对照组、荷S180肉瘤模型组、模型 +环己亚硝脲组和模型 +脑瘤消小、中、大剂量组。小鼠皮下接种S180肉瘤后开始灌胃给药 ,每天一次 ,连续 8天。停药后次日处死小鼠 ,称瘤重后计算瘤重抑制百分率 ,并对瘤组织进行病理学检查 ,实验共重复三次。结果　与模型组 (ig生理盐水 )比较 ,脑瘤消胶囊各剂量组小鼠肿瘤明显缩小 (P <0 .0 1)。结论　 0 .5～ 2 .0g/kg脑瘤消胶囊灌胃给药可明显抑制小鼠荷S180实体瘤的生长和浸润。     

Vitamin E protects against the mitochondrial damage caused by cyclosporin A in LLC-PK1 cells

Cyclosporin A (CsA) has nephrotoxic effects known to involve reactive oxygen species (ROS), since antioxidants prevent the kidney damage induced by this drug. Given that mitochondria are among the main sources of intracellular ROS, the aims of our study were to examine the mitochondrial effects of CsA in the porcine renal endothelial cell line LLC-PK1 and the influence of the antioxidant Vitamin E (Vit E).Following the treatment of LLC-PK1 cells with CsA, we assessed the mitochondrial synthesis of superoxide anion, permeability transition pore opening, mitochondrial membrane potential, cardiolipin peroxidation, cytochrome c release and cellular apoptosis, using flow cytometry and confocal microscopy procedures. Similar experiments were done after Vit E preincubation of cells.CsA treatment increased superoxide anion in a dose-dependent way. CsA opened the permeability transition pores, caused Bax migration to mitochondria, and decreased mitochondrial membrane potential and cardiolipin content. Also CsA released cytochrome c into cytosol and provoked cellular apoptosis. Vit E pretreatment inhibited the effects that CsA induced on mitochondrial structure and function in LLC-PK1 cells and avoided apoptosis.CsA modifies mitochondrial LLC-PK1 cell physiology with loss of negative electrochemical gradient across the inner mitochondrial membrane and increased lipid peroxidation. These features are related to apoptosis and can explain the cellular damage that CsA induces. As Vit E inhibited these effects, our results suggest that they were mediated by an increase in ROS production by mitochondria.     

脑平衡仿生仪对肾型加压素性高血压大鼠单胺神经递质的影响

目的：进一步证实脑平衡仿生仪（简称NTJ仪）调节中枢治疗高血压的机制。方法：采用夹肾动脉并静脉加压素的方法制备大鼠肾型加压素性高血压模型，观察NTJ（Nao-Tai-Ji)仪对模型大鼠血压及下丘脑单胺类神经递质的影响。结果：NTJ仪对大鼠急性高血压模型有明显的急性降压作用（P＜0．05），能使血压降至接近正常水平，但对正常的大鼠血压无明显影响。NTJ仪治疗后能使模型动物下丘脑内的去甲肾上腺素（NE）含量下降（P＜0．05）。但对5－羟色胺（5－HT）和多巴胺（DA）水平无明显影响。结论：NTJ仪降压可能机理是通过生物信息反馈方式，改善大脑皮层兴奋与抑制的平衡，激发大脑神经递质的潜能，降低下丘脑的中枢神经递质NE含量，影响下丘脑血管舒缩中枢的功能，从中枢向外周释放抑制信息，降低交感神经兴奋度，减少外周血管阻力，达到快速降低血压的效果。     

抗脑瘤口服液治疗脑瘤前后脑电地形图研究

目的：探讨脑电地形图在脑瘤疗效判断上的应用价值。方法：通过对30例脑瘤治疗前后脑电地形图改变进行对比评价。结果：脑电地形图在治疗前后有明显改变。结论：脑电地形图可以作为脑瘤疗效观察的一个有效指标。     

Central role of mitochondria and p53 in PUVA-induced apoptosis in human keratinocytes cell line NCTC-2544

Despite strong evidence concerning the high efficiency of PUVA therapy (psoralen plus UVA light), its mechanism of action has not yet been fully elucidated. In this study, we have evaluated in a cell line of human keratinocytes (NCTC-2544) the effects of two linear psoralen derivatives, 8-methoxypsoralen (8-MOP) and 5-methoxypsoralen (5-MOP), that are widely used in PUVA therapy and two angular derivatives, Angelicin (ANG) and 4,6,4'-trymetyl angelicin (TMA). All derivatives photoinduce cellular death, TMA being the most active compound. The cell cycle analysis showed that the four derivatives induce, 24 h after irradiation, a cell cycle arrest in G1 phase later followed by massive apoptosis. The G1 arrest is correlated to an increase in the expression of p21(Waf1/Cip1), a protein associated with the cell cycle block and apoptosis. Furthermore, treatment of NCTC-2544 resulted in p53 activation by 5-MOP, 8-MOP, and ANG but not TMA and its phosphorylation at serine-15. The levels of p21(Waf1/Cip1) paralleled p53 protein staining pattern suggesting that p53 activation correlated with p21(Waf1/Cip1) induction. Simultaneous to p53 activation, psoralens induced mitochondrial depolarization, cytochrome c release, mitochondrial production of reactive oxygen species, as well as caspase-3 and -9 activation. Thus these results strongly indicate the necessity of p53 activation and the induction of the apoptotic machinery downstream of mitochondria.