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Antimycin A (AMA) is an inhibitor of mitochondrial electron transport via its binding to complex III. In the present study, the mechanisms involved in AMA-induced cell damage were investigated. Treatment of osteoblastic MC3T3-E1 cells with AMA decreased adenosine 3′,5′-cyclic monophosphate (cAMP) level, activities of phosphoinositide 3-kinase (PI3K) and Akt (protein kinase B), and phosphorylated CREB (cAMP-response element-binding protein). To examine whether AMA-induced cell damage involves altered metabolism of pyridine nucleotides, the levels of NAD+, NADH, NADP+, and NADPH were measured. Treatment with AMA significantly decreased the levels of NAD+ and NADPH. Moreover, the activities of aconitase and thioredoxin reductase were decreased by AMA treatment. These results suggest that PI3K/Akt/CREB pathway and pyridine nucleotide (NAD+ and NADPH) are related to mitochondria function of osteoblasts.  相似文献   
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成都市新生儿葡萄糖-6-磷酸脱氢酶缺乏症筛查分析   总被引:1,自引:0,他引:1  
目的 了解成都地区新生儿葡萄糖-6-磷酸脱氢酶(G6PD)缺乏症的发病分布情况.方法 采用NADP+氧化还原酶法,对11 085例(男6 440例,女4 645例)就诊患儿进行红细胞中G6PD活性的定量检测.结果 在11 085例受检者中,检出G6PD缺乏者677例,总检出率6.1%.其中男婴545例,占男婴检查人数的8.46%(545/6 440);女婴132例,占女婴检查人数的2.84%(132/4 645).结论 成都地区G6PD缺乏症检出率较高,应在新生儿期常规开展G6PD检测,及早诊断,采取有效防范措施,预防由G6PD缺乏引起的新生儿高胆红素血症及核黄疸的发生.  相似文献   
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基于PNO基因序列分析隐孢子虫种系发育关系   总被引:1,自引:0,他引:1  
本文以核基因组的功能蛋白丙酮酸∶NADP^+氧化还原酶(pyruvate∶NADP^+ oxidoreductase,PNO)编码基因作为研究对象,对本实验室分离保存的隐孢子虫虫株进行扩增测序,用ClustalX 1.81对扩增序列与GenBank相关参考序列进行比对,用Paup4.0程序中邻接法(Neighbor-joining method,NJ)、最大简约法(Parsimony,MP)和最大似然法(Maximum Likelihood,ML)进行聚类分析,以确定不同隐孢子虫分离株之间的遗传进化关系,并以18S rRNA和HSP70基因构僵的基因树作参照,进而评价PNO这一基因座是否适合作为隐孢子虫基因分型和进化关系分析的基因座。结果表明通过PNO构建的进化树将隐孢子虫分为2大类:Cryptosporidium bailey和C.meleagridis处于一个分枝,C.hominis、C.parvum牛基因型和C.parvum鼠基因型处于另一个分枝上。不同隐孢子虫之间的同源性介于95.0%-100%,能有效区分隐孢子虫不同基因型。因此,PNO基因序列也适合作为隐孢子虫分离株种系发育的遗传标记。  相似文献   
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Propolis is a substance produced by honeybees (Apis mellifera L.). Its components are strong antioxidants and free radical scavengers. The aim of this study was to evaluate the protective effects of a water extract of Brazilian green propolis (WEP) combined with the antitumor agent doxorubicin (DXR) on Drosophila melanogaster wing cells through the somatic mutation and recombination test (SMART). Two different crosses were used: The standard (ST) cross and the high bioactivation (HB) cross. The HB cross is characterized by a constitutively enhanced level of cytochrome P450 which leads to an increased sensitivity to a number of promutagens and procarcinogens. Larvae obtained from these two crosses were chronically treated with different concentrations of WEP (12.5,25.0 and 50.0 mg/mL) alone or combined with DXR (0.125 mg/mL). The results obtained with the two different crosses were rather similar. Neither toxicity nor genotoxicity were observed in WEP treated series. Simultaneous treatment with different concentrations of WEP and DXR led to a reduction in the frequency of recombination compared to the treatment with DXR alone. This anti-recombinogenic effect was proportional to the concentrations applied, indicating a dose-response correlation and can be attributed to the powerful scavenger ability of WEP.  相似文献   
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The antioxidant activity of vegetables subjected to minimal processing (in MAP, and intended for cooking or for use in salads), dehydrated condiments and ready-to-eat vegetables such as soups and purees, was assessed by reference to their ability to scavenge lipoperoxyl and hydroxyl radicals and Trolox-equivalent antioxidant capacity. In the case, the MAP vegetables the measurements were repeated during eight days of storage in a domestic refrigerator and after cooking (boiling, microwaving, pressure cooking, griddling, frying and baking). MAP vegetables had a good or very good antioxidant capacity, and showed no significant loss of antioxidant activity or scavenging capacity compared with fresh vegetables. The cooking treatments that keep the antioxidant activity of MAP vegetables are microwaving, sautéing and baking. The most aggressive method of cooking were steaming, boiling and frying. The dehydrated condiments (tablets) showed higher antioxidant activity than the ready-to-eat soup. The enrichment of stews and casseroles, with dehydrated vegetable tablets, and the consumption of soup or vegetable purees represent an increased antioxidant intake in our diet. Also “ready-to-eat” vegetable soups show antioxidant activity after they have been submitted to heat treatment to increase their shelf-life. They can be recommended as alternatives in our non-stop “life style”.  相似文献   
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Alzheimer's disease (AD) is the most common form of dementia and the most common neurodegenerative disease, with a complex genetic background. Genome-wide association studies (GWAS) have yielded important new insights into genetic mechanisms of AD pathology. Current results unequivocally confirm apolipoprotein E (APOE) as a major genetic risk factor for development of late onset AD. Additional associations of more than twenty genes have also been identified and replicated in subsequent genetic studies. Despite the exciting new GWAS data which have emerged in the last few years, it has become clear that common variants within the genome cannot fully explain the underlying genetic risk for AD. Novel approaches such as genome-wide analysis of copy number variations (CNV) or low-frequency rare functional gene variants may provide additional insight into genetic basis of AD. In this review we summarize the findings of eighteen GWAS studies in AD performed to date, with an emphasis on potential future developments in the quest for genetic risk factors of AD.  相似文献   
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