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1.
目的:探讨藏药湿生扁蕾提取物通过 NF -κB 信号通路诱导结肠癌 SW480细胞凋亡的作用及其机制。方法本研究用不同浓度的藏药湿生扁蕾提取物处理结肠癌 SW480细胞,通过 JC -1染色检测 SW480细胞线粒体膜电位的变化,通过 Hoechst 染色以判断藏药湿生扁蕾提取物是否具有诱导结肠癌细胞 SW480细胞凋亡效应。同时检测 NF -κB 蛋白的表达,用以明确 NF -κB 信号通路在结肠癌发生中的作用机制。结果经一定浓度的湿生扁蕾提取物处理后的 SW480细胞内,NF -κB 的表达明显下调,染色现象揭示湿生扁蕾提取物具有促进肿瘤细胞凋亡的作用。结论湿生扁蕾提取物可以在体外诱导肿瘤细胞凋亡,其抗肿瘤机制是通过 NF -κB 信号通路介导的。 相似文献
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目的选择肉苁蓉最佳提取工艺参数,研究不同干燥方式对5种苯乙醇苷类成分的影响。方法采用单因素筛选结合Box-Behnken响应面法优化提取工艺。最优条件提取后,利用HPLC法检测不同干燥方式肉苁蓉的松果菊苷、肉苁蓉苷A、毛蕊花糖苷、异毛蕊花糖苷、2′-乙酰基毛蕊花糖苷成分含量并采用单因素方差分析、聚类分析、主成分分析、密切值法分析5种苯乙醇苷类成分含量,选择最佳干燥方式。结果最佳提取工艺:甲醇体积分数55.14%,液料比46.39,提取时间38.50 min。聚类分析、主成分分析、密切值法分析表明,采用冷冻干燥法所得到的肉苁蓉品质最好,80℃烘干次之,40℃烘干最低。结论使用该工艺提取肉苁蓉,其5种苯乙醇苷类成分提取完全、充分。虽然冷冻干燥法炮制肉苁蓉所保留有效成分最高,但从生产角度考虑,采用80℃烘干法炮制能达到成本和功效的平衡。 相似文献
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Determination of Clinical Cut-Off Values for Serum Cystatin C Levels to Predict Ischemic Stroke Risk
Youyi Wang Ying Zhang Qinghua Ma Congju Wang Yong Xu Hongpeng Sun Yana Ma 《Journal of stroke and cerebrovascular diseases》2019,28(11):104345
Background: The association between cystatin C and risk of ischemic stroke is inconsistent and the cut-off values of cystatin C are diverse in different articles. We aimed to investigate the association between cystatin C levels and the development of ischemic stroke and to explore the clinical cut-off values of serum cystatin C levels for ischemic stroke. Methods: This prospective cohort study included 7658 participants from the China Health and Retirement Longitudinal Study who were free of cardiovascular diseases and cancer at baseline. A decision-tree model was used to find reasonable cut-off values for cystatin C levels. Logistic regression models were used to analyze the association between different levels of cystatin C and the risk of ischemic stroke. Results: The whole cohort was divided into the following 3 groups according to the decision tree: group-low (<.901 mg/L), group-moderate (.901~1.235 mg/L), and group-high (>1.235 mg/L). After 4 years of follow-up, we identified 156 cases of ischemic stroke. After adjusting for potential confounding factors, the odds ratios (95% confidence intervals) of ischemic stroke were 1.637 (1.048-2.556) for group-moderate and 2.326 (1.285-4.210) for group-high) compared with the low group of cystatin C. Subgroup analyses showed that the association between cystatin C levels and the incidence of ischemic stroke was more pronounced in males or old people than in females or young people. Conclusions: We found 2 suitable cut-off values for serum cystatin C levels and found that high levels of cystatin C were associated with an increased risk of ischemic stroke. 相似文献
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[目的]探讨马大正老师治疗各型经行头痛的经验。[方法]通过分析马大正老师对经行头痛病因病机的认识、总结马老的临证思路和典型案例,来阐述马大正老师治疗妇科经行头痛的临床经验。[结果]经过马大正老师的中医辨证方法拟方,分别以外感风邪、肝火上逆、血虚生风、痰浊内阻、肝胃不和、气滞血瘀等治疗各型经行头痛,临床取得良好的效果。[结论]引起经行头痛的病因很多,但马老根据患者的特点辨证论治选方用药精准能取得很好的疗效,对临床治疗经行头痛有很好的指导作用。 相似文献
6.
Pearl Ma Aaron Lloyd Morgan McGrath Ariel Shuchleib Cung Ikemefuna Akusoba Alice Jackson Daniel Swartz Keith Boone Kelvin Higa 《Surgery for obesity and related diseases》2019,15(9):1554-1562
BackgroundUse of liposomal bupivacaine (LB) in surgery is reported with decreased postoperative opioid requirements. The efficacy of LB versus standard bupivacaine injections at laparoscopic port sites during bariatric surgery is unknown.ObjectivesTo determine whether there was a difference in postoperative hospital opioid requirements after port site injections of LB versus standard bupivacaine during laparoscopic bariatric surgeries. Primary endpoint was total in hospital opioid use expressed as morphine-equivalent use. Secondary endpoints included home opioid use, pain scores, hospital length of stay, and adverse events.SettingAcademic-affiliated private practice.MethodsA 2-group randomized, double-blinded trial from November 2017 to August 2018 with patients randomly assigned to receive either LB or bupivacaine alone at trocar site injections during laparoscopic Roux-en-Y gastric bypass (LRYGB) or vertical sleeve gastrectomy (VSG). All patients underwent enhanced recovery after bariatric surgery protocols.ResultsAll patients undergoing LRYGB or VSG assessed for eligibility. Of 682 patients undergoing LRYGB or VSG, 231 met inclusion criteria, 52 patients excluded intraoperatively. Among 231 patients (mean age, 39.2 years; 79% women; mean body mass index 45.0), 179 patients (77%) completed the trial. Patients randomly assigned to receive either LB (n = 89) or bupivacaine alone (n = 90) at trocar site injection during LRYGB or VSG. Postoperative morphine-equivalent use were similar (LB 8.3 [standard deviation 4.0–13.9] versus bupivacaine group 7.5 [standard deviation 3.6–13.1] P = .94) with highest requirement in first 4 hours after surgery. There was no significant difference in length of stay, pain scores, or complications. There were more patients in the bupivacaine group that did not take pain medications on postoperative days 2 to 4 (P = .032, P = .23, P = .005, respectively). There were more patients in the bupivacaine group 48.1% (n = 39) compared with the LB group 34.2% (n = 27) that did not consume any narcotic tablets at home but this not found to be statistically significant (P value = .07).ConclusionsAmong patients undergoing primary bariatric surgery under enhanced recovery after bariatric surgery protocol, there was no significant difference in postoperative hospital opioid use in those receiving LB compared with standard bupivacaine. A greater percentage of patients in the standard bupivacaine group did not require any narcotics at home, which was significant on postoperative days 2 to 4. To become completely opioid free after bariatric surgery, resources should be focused on multimodal approaches instead of reliance on type of anesthetic medication used. 相似文献
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盂河医派是近代中医药最有影响的一大流派,以其和法缓治的医疗风格而著称。该文以孟河医家的传世著作中有关咳嗽的医论、医案为研究对象,从理、法、方、药等方面,分析孟河医家费伯雄、马培之、丁甘仁治疗咳嗽的主要经验。 相似文献
9.
目的分析安徽马鞍山市辖区近年来血吸虫病疫情变化趋势,评估到2015年实现血吸虫病传播阻断目标风险。方法 2009~2013年期间、通过免疫学和病原学相结合的方法开展人畜病情监测;采用系统抽样结合环境抽查法开展螺情监测;同时,通过人畜化疗和钉螺控制措施开展血吸虫病防控工作。结果 2009~2013年,人群治疗及扩大化疗2 060人次,采用IHA法共筛查20 622人次,血检阳性率平均为3.75%,处于徘徊稳定状态;kato法粪检查病1 526人次,均未查出阳性。5年间家畜扩大化疗279头次,家畜查病231头次,均未查出阳性。2009~2013年,开展药物灭螺611.3 hm~2,环境改造灭螺10.7 hm~2,开展螺情监测3 152 hm~2,均未查获感染性钉螺;活螺密度从2009年0.205只/0.11m~2下降到2013年的0.0075只/0.11m~2,呈现逐年下降趋势,有螺面积比2009年下降44.1%。结论 2009年以来,马鞍山市辖区血吸虫病疫情呈现稳中有降的趋势,5年间均未查出病人、病畜和感染性钉螺,活螺密度和有螺面积逐年下降,距离血吸虫病传播阻断目标差距逐渐缩小。目前主要障碍仍然是辖区尚有较大面积的钉螺分布环境。 相似文献
10.
目的:评价麻杏石甘汤加减结合沙美特罗氟替卡松吸入治疗慢性阻塞性肺疾病(chronic obstructive pulmonary disease, COPD)急性加重的疗效。方法将86例符合入选标准的COPD急性加重患者按随机数字表法分为沙美特罗氟替卡松组(41例)和联合治疗组(45例)。沙美特罗氟替卡松组给予沙美特罗氟替卡松吸入治疗,联合治疗组在此基础上加用麻杏石甘汤加减治疗。采用免疫散射比浊法测定血清CRP水平,流式细胞术检测血细胞Toll样受体9(Toll-like receptor 9, TLR9)表达。评价咳嗽、痰量、喘息和气短的中医证候积分、肺功能和临床疗效。结果治疗后,联合治疗组血清CRP[(4.3±1.2)mg/L比(8.4±2.5)mg/L;t=5.417,P<0.01]显著低于沙美特罗氟替卡松组,血细胞 TLR9表达[(1.9±0.7)比(1.6±0.4);t=3.418,P<0.05]显著高于沙美特罗氟替卡松组;联合治疗组咳嗽[(1.7±0.6)分比(3.8±1.1)分;t=2.859,P<0.05]、痰量[(1.6±0.4)分比(3.9±1.2)分;t=3.027,P<0.05]、喘息[(1.2±0.5)分比(3.4±1.3)分;t=3.416,P<0.05]和气短[(1.5±0.7)分比(3.7±1.6)分;t=3.468,P<0.05]评分均显著低于沙美特罗氟替卡松组。联合治疗组第一秒用力呼气量[(75.4±5.8)L比(62.8±6.9)L;t=3.526,P<0.05]、第一秒用力呼气量占用力肺活量百分率[(85.7±10.3)%比(71.9±15.4)%;t=5.648,P<0.01]显著高于沙美特罗氟替卡松组。联合治疗组症状缓解时间[(3.4±0.7)d 比(5.6±1.2)d;t=3.256,P<0.05]显著短于沙美特罗氟替卡松组,应用沙美特罗氟替卡松的剂量[(1.8±0.2)×103μg比(5.3±0.4)×103μg;t=5.627, P<0.05]和次数[(7.4±1.3)次比(16.5±3.4)次;t=4.574,P<0.05]均较沙美特罗氟替卡松组显著缩短和降低。联合治疗组总有效率显著高于沙美特罗氟替卡松组[84.4%(38/45)比73.2%(30/41);χ2=4.519,P<0.05]。结论麻杏石甘汤加减结合沙美特罗氟替卡松吸入可改善COPD急性加重患者的肺功能,疗效优于单用沙美特罗氟替卡松吸入。 相似文献