补肾益气活血法对应激、老年小鼠学习与记忆及老化相关酶活性的影响

目的:探讨补肾益气活血方-葆青康对应激、老年小鼠学习与记忆及相关酶活性的影响。方法:采用游泳实验、耐缺氧实验和耐高温实验观察葆青康对小鼠抗应激能力的提升作用;采用食饵法大迷宫法测定葆青康对中、老年小鼠学习与记忆的影响;采用McEwen法测定葆青康对中、老年小鼠脑中单胺氧化酶-B(MAO-B)活性的影响;采用TBA比色法、邻苯三酚自氧化法和DNBT法测定葆青康对老年小鼠肝丙二醛(MDA)含量,心、脑、肝和血中超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-Px)活性的影响。结果:葆青康能延长小鼠游泳时间、增加常压缺氧小鼠存活时间和降低小鼠耐高温的死亡率,提高小鼠抗应激的能力;葆青康能增加中老年小鼠学习与记忆能力,降低中老年小鼠脑MAO-B活性,降低老年小鼠肝中MDA含量、提高老年小鼠心、脑、肝和血中SOD和GSH-Px活性。结论:葆青康具有抗应激和抗衰老作用。     

白芍水煎剂对老龄小鼠抗衰老作用的实验研究

中药白芍水煎剂给予老龄小鼠灌胃(10g/kg),每日一次,连用4周。采用邻苯三酚自氧化法、DTNB和TBA比色法测定红细胞超氧化物歧化酶(SOD)活性,全血谷胱甘肽过氧化物酶(GSH-Px)活力和红细胞中及血浆中MDA含量。结果表明,白芍水煎剂能显著增强老龄小鼠红细胞SOD活性、全血GSH-Px的活力,而且可显著降低红细胞和血浆中MDA含量,故具有延缓衰老作用。     

硒缺乏降低大鼠组织中5′-脱碘酶活性

用不同硒含量的半合成饲料(试验组饲料含硒0.005ppm,对照组饲料含硒0.105ppm),饲喂断奶的Hooded Lister大鼠6周,研究了在不同硒营养状态下,大鼠肝、肾、脑、垂体及褐脂中5′-脱碘酶活性的变化。试验进行2、4、6周后,试验组大鼠组织中GSH-Px及5′-脱碘酶活性比对照组显著降低。按组织中GSH-Px及5′-脱碘酶活性下降幅度的大小,可把各组织分别排序为:肝>肾>褐脂>垂体>脑和肝>肾>褐脂>脑>垂体。硒耗竭2、4、6周后,肝脏GSH-Px活性下降94％、99％、及99.2％,肝脏5′-脱碘酶活性下降的幅度分别为62％80％及90％。硒调节5′-脱碘酶活性可能因为硒是Ⅰ型脱碘酶的辅助因子;而Ⅱ型脱碘酶活性的变化则可能是缺硒导致组织局部T_4水平升高的间接作用。硒缺乏降低5′-脱碘酶活性会导致大鼠循环T_4水平升高,而循环T_3水平降低。     

Lipid peroxidation and antioxidant status in human cervical carcinoma

Reactive oxygen species (ROS), represented by superoxide, hydrogen peroxide and hydroxyl radicals, have been implicated in many diseases including cancer. ROS have been known to play an important role in the initiation and promotion of multistep carcinogenesis. The cellular antioxidants play a crucial role in protection against neoplastic disease. However, very little is known about the antioxidant defense in cervical carcinoma. This is addressed in the present study. Lipid peroxides, glutathione content and the activities of antioxidant enzymes, together with vitamin C and E content, were estimated in patients who had carcinoma of the cervix, and the values were compared with those of normal women. The results showed a remarkable reduction in the content of glutathione, vitamin E and C. Activities of glutathione peroxidase and superoxide dismutase were also reduced in cervical cancer compared to normal controls (P < 0.001). This reduction was more marked in late stages (III, IV) than in early stages (I, II) (P < 0.001). Glutathione was reduced more in poorly differentiated tumors (grade III) than in well and moderately differentiated ones (grade I, II) (P < 0.05). Levels of lipid peroxides were found to be significantly higher in malignant than in normal tissue samples and their levels were correlated with advanced clinical stage (P < 0.001). Our results suggest impaired antioxidant status in carcinoma of the cervix. This impairment is related to tumor progression.     

Effects of ligustrazin on the gastrointestinal tract after severe scald in rats

To observe the effects of ligustrazin on the contents of ATPase, GSH-Px, MDA in stomach and intestine of severely burned rats. *Divide the rats randomly into three groups. Tissue of stomach and intestine were gathered and homogenized respectively, and contents of MDA, LDH, GSH-Px, SOD and ATPase were assayed. The contents of GSH-Px, SOD and ATPase in stomach and intestine were progressively decreased while MDA and LDH obviously enhanced in comparison with that of normal group. After the treatment with ligustrazin, the contents of GSH- Px, SOD and ATPase were significantly higher but MDA and LDH lower than that of non - treated burned group. So ligustrazin have protective effects on stomach and intestine in burned rots. It reduces free radical and has antioxidant effect on stomach and intestine.     

EFFECTS OF COMBINATION OF ACUPUNCTURE AND MOXIBUSTION WITH CHINESE DRUGS ON LIPIDS PEROXIDE AND ANTIOXIDASE IN PATIENTS OF VASCULAR DEMENTIA

Withincreaseofincidenceofcerebrovascu-lardiseases,thepatientsofVDincreasedaybyday.Inrecentyearsithasbeenindicatedthatacupuncture,moxibustionandChinesedrugshavedefinitetherapeuticeffectsforVDL1].Inordertoprobeintothetherapeuticmechanism,inthepresentexperim…     

维生素C、维生素E、硒与镉联合作用对LLC-PK1细胞脂质过氧化的影响

[目的]研究氯化镉(CACl2)及其分别与维生素C(VitC)、维生素E(VitE)、硒联合作用对猪肾近曲小管上皮(LLC—PKl)细胞增殖、脂质过氧化及抗氧化酶的影响。[方法]实验设对照组、镉组、镉 Vitc组、镉 VitE组和镉 硒组。以四甲基偶氮唑盐(MTT)比色法检测LIE—PK,细胞活力的变化，黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活力，以催化还原性谷胱甘肽(GSH)速度表示谷胱甘肽过氧化物酶(GSH—Px)的活力，硫代巴比妥酸法(TBA)测定丙二醛(MDA)含量。[结果]不同浓度cdcl2(0—100μmol／L)作用于LIE—PK1细胞12h，细胞存活率随CAC12浓度升高而下降，呈剂量一反应关系；VitC 镉组、vitE 镉组、硒 镉组分别与单独加镉组相比均可显著提高LIE—PK,细胞存活率；10—40pmol／L(ktCl2作用LLC—PK，细胞12h后的培养液上清MDA含量明显增加，与对照组相比差别有显著性。同时，CuZn-SOD、Mn-SOD、GSH—Px活力较对照组相比显著性升高；硒 镉组细胞培养液上清MDA含量与镉组相比有显著性下降。[结论]镉对LIE—PKl细胞增殖有明显的抑制作用；镉可引起LIE．PK1细胞脂质过氧化，是导致肾细胞毒性机制之一；硒对镉引起的ILC—PK。细胞的脂质过氧化有一定的拮抗作用。     

三种饮用水对小鼠抗过氧化酶影响比较

目的比较不同饮用水对小鼠的抗过氧化酶的影响。方法ICR小鼠随机分为三组,每组雌雄各半,分别用矿泉水、自来水和纯净水喂养。经过一年时间喂养后,取其肺、肝、脑、心制成匀浆测定谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(SOD)活力。结果矿泉水组小鼠的心匀浆GSH-Px活力较纯净水组高,有显著差异(P<0.05);而SOD活力矿泉水组除在肺匀浆中明显高于其他两组(P<0.05),肝中SOD纯净水组高于矿泉水组。结论不同饮用水对小鼠组织器官的抗氧化酶影响不同,表现出不同的抗氧化/致氧化效应。     

大豆黄酮抗热应激作用

目的研究大豆黄酮对热应激小鼠谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量的影响。方法将30只小鼠随机分为正常对照组、热应激组和大豆黄酮热应激组,以(45±1)℃持续20 min的热应激模式,观察热应激后小鼠肝、脾、肾及血清中GSH-Px活力及MDA含量变化。结果与正常对照组相比,热应激后小鼠肝脏、脾脏及肾脏GSH-Px活力升高,其中肝脏酶活力显著升高(P<0.05);大豆黄酮热应激组酶活力显著高于正常对照组(P<0.05或P<0.01),肝脏、脾脏酶活力显著高于应激组(P<0.05)。热应激后2组MDA含量均比正常对照组升高,其中肝脏和血清上升显著(P<0.05);大豆黄酮热应激组比应激组有所下降,但差异无统计学意义(P>0.05)。结论大豆黄酮可增强应激机体的抗氧化能力。     

ROS指标在保健食品抗氧化功能检测中的应用及灵敏性比较

目的：将ROS指标应用于保健食品抗氧化功能检测中，并进行敏感性比较。方法：SPF级昆明种雄性小鼠，12月龄，按体重随机分为老龄组对照组、大豆异黄酮低、中、高剂量组（分别为0．08g／kgBW、0．17g／kgBW、0．50g／kgBW），每组10只动物。连续灌胃给予受试物42d后，断头处死动物，取肝脏。以胶原酶和胰蛋白酶联合酶解肝组织，制备肝组织单细胞悬液；以流式细胞术测定肝细胞ROS水平；同时制备肝组织匀浆，以分光光度法测定肝细胞MBA含量及SOD、GSH—Px活性。结果：流式仪直方图分析显示，随着受试物给予剂量的增加，各剂量组肝细胞ROS水平均明显降低（P〈0．05）；低、高剂量组肝细胞内MDA含量明显减少（P〈0．05），中、高剂量组GSH—Px活性明显增加（P〈0．05），高剂量组,SOD活性亦明显增加（P〈0．05）；其余剂量组各指标差异无统计学意义（P〉0．05）。结论：大豆异黄酮通过降低肝细胞自由基水平而起到抗氧化作用，以FCM检测肝细胞ROS水平可更好地对保健食品的抗氧化功能进行筛选与评价。