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1.
目的:探讨肝细胞肝癌(hepatocellar carcinoma,HCC)中c-Met、Fascin及CD44与临床病理特征及预后之间的关系。方法:通过免疫组织化学法(immunohistochemistry,IHC)检测186例HCC患者中HCC组织及癌旁组织c-Met、Fascin及CD44蛋白的表达, 分析三者与临床病理特征及预后的关系。采用SPSS 21.0软件进行统计分析。结果:在HCC组织及癌旁组织中,c-Met表达阳性率分别为60.2%(112/186)和32.8%(61/186),Fascin表达阳性率分别为56.5%(105/186)和24.7%(46/186),CD44表达阳性率分别为73.1%(136/186)和50.5%(94/186),三种蛋白在HCC组织中的表达显著高于癌旁组织,(分别χ2=28.1050,χ2=38.8041,χ2=20.0921,均P<0.01)。c-Met、Fascin及CD44蛋白的表达与脉管侵犯、低分化及术后复发有关(c-Met分别χ2= 5.4762,χ2=14.6101 ,χ2= 8.6439,Fascin分别χ2= 7.4015,χ2=28.3361,χ2=13.8529,CD44分别χ2=11.3806,χ2=21.2081,χ2= 15.5342,均P<0.05)。相关性分析显示c-Met与Fascin 、c-Met与CD44的表达均呈显著正相关(分别为r=0.3495,0.2009,P均<0. 01) 。Fascin与CD44表达的相关性无统计学意义(r=-0.0679,P>0. 05)。单因素和Cox模型多因素结果显示c-Met、Fascin及CD44表达是HCC预后的独立危险因素(均P<0. 05)。结论:在HCC组织中,c-Met、Fascin及CD44蛋白的表达均增高,同时c-Met与Fascin、c-Met与CD44蛋白表达均呈正相关,且三者的高表达分别与癌组织分化、脉管癌栓及复发有关,通过IHC检测三者的表达具有判断HCC预后的价值。 相似文献
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Pallawi Torka MD Othman S. Akhtar MD Nishitha M. Reddy MD Bora E. Baysal MD PhD Angela Kader BA CCRP Adrienne Groman MS Jenna Nichols RN MS CCRC Cory Mavis BS MS Joseph D. Tario PhD AnneMarie W. Block PhD Sheila N. J. Sait PhD Paola Ghione MD Suchitra Sundaram MD Eugene R. Przespolewski PharmD BCOP Alice Mohr FNP-C MSN Ian Lund PA-C Jessica Kostrewa NP Kenneth McWhite PA Joseph DeMarco PA Michael Johnson DNP BSN NP RN Andrea Darrall NP Rosh-Neke Thomas-Talley FNP-BC Paul K. Wallace PhD Vishala Neppalli MD Alan Hutson PhD Francisco J. Hernandez-Ilizaliturri MD 《Cancer》2022,128(8):1595-1604
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《Transfusion and apheresis science》2022,61(2):103327
BackgroundPlatelet transfusion therapy is widely used to prevent hemorrhage in patients with thrombocytopenia and platelet disorders. The platelet concentrate (PC) quality is affected by increased storage time, as reflected in the decreased number of platelets, morphological changes, and impaired functions. This study aimed to analyze the impact of 5 days storage on platelets count and the expression of CD63, and Annexin V as activation markers during PC storage.MethodsFifty PCs collected from single donors were tested for platelet count on days 0, 3, and 5 using a Sysmex blood counter. CD61, CD63, and Annexin V expression was analyzed by a multicolor Navios flow cytometer.ResultsThere was a significant decrease in platelet count during 5 days of storage. There was a direct relationship between storage time and degree of platelet activation. CD63 had almost double increased expression on day 5 than day 3. Annexin V showed significantly increased expression on day 3 with minor differences between days 3 and 5.ConclusionAccording to standard blood bank conditions, PC stored for 5 days showed a degree of in vitro activation as evidenced by CD63 and Annexin V expression, may lead to reduced therapeutic efficacy. Flow cytometry monitoring platelet activation in PC offers a better understanding of the changes during PC storage and may help improve platelet products. 相似文献
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目的分析肝移植术后受者外周血CD19+CD24hiCD38hi B细胞占单个核细胞比例变化情况及其与急性细胞性排斥反应(ACR)之间的关系。 方法回顾性分析2013年12月至2015年12月在浙江大学医学院附属第一医院接受心脏死亡器官捐献肝移植的80例成人受者临床资料,根据术后是否发生ACR,将受者分为ACR组(25例)和非ACR组(55例)。术前、术后各个时间点抽取参加研究者静脉血并分离外周血单个核细胞,加入异硫氰酸荧光素-单克隆鼠抗人CD19抗体、藻红蛋白-单克隆鼠抗人CD24抗体和别藻蓝蛋白-单克隆鼠抗人CD38抗体,流式细胞仪检测各组CD19+CD24hiCD38hi B细胞百分比。采用t检验和单因素方差分析比较正态分布计量资料,采用χ2检验比较计数资料,采用Kaplan-Meier法绘制受试者工作特征曲线(ROC曲线)。P<0.05为差异有统计学意义。 结果ACR组、非ACR组受者术前外周血平均CD19+CD24hiCD38hi B细胞比例分别为(3.13±0.91)%、(3.49±0.83)%,差异无统计学意义(t=1.636,P>0.05)。ACR组术后发生ACR前外周血平均CD19+CD24hiCD38hi B细胞比例为(1.87±0.70)%。非ACR组受者术后3个月、6个月和1年外周血平均CD19+CD24hiCD38hi B细胞比例分别为(1.64±0.52)%、(1.63±0.56)%和(2.04±1.24)%,术后3、6个月平均值均低于术前和术后1年,差异均有统计学意义(P均<0.05)。ACR组受者发生ACR时外周血平均CD19+CD24hiCD38hi B细胞比例为(0.8±0.5)%,低于发生ACR前的平均水平(t=5.752,P<0.05),且低于非ACR组术后3个月、6个月和1年的平均水平(P<0.05)。ACR组受者接受抗排斥反应治疗后,CD19+CD24hiCD38hi B细胞比例也逐渐增加,ACR发生后7 d为(0.84±0.08)%,与ACR发生时相比差异无统计学意义(P>0.05);而发生30 d后达(1.65±0.18)%,与ACR发生时相比差异有统计学意义(P<0.05)。当截断值为1.015%时,CD19+CD24hiCD38hi B细胞比例预测ACR发生的敏感度和特异度分别为0.786和0.702,ROC曲线下面积为0.775(95%CI: 0.671~0.879,P<0.05)。 结论CD19+CD24hiCD38hi B细胞比例下降与肝移植术后ACR反应发生有关,并可作为预测ACR发生的细胞标志物。 相似文献
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Effect of Replacement of Wharton Acellular Jelly With FBS on the Expression of Megakaryocyte Linear Markers in Hematopoietic Stem Cells CD34+ 下载免费PDF全文
zahra Jalili Behnam Emamgolizadeh Hossein Abbaszadeh Shahla JaliliMehdi Derakhshani Mehdi YousefiMehdi TalebiKarim Shams Asenjan Ali Akabr Movassaghpour 《Asian Pacific journal of cancer prevention》2022,23(10):3281-3286
Objective: Animal environments for the growth of stem cells cause the transmission of some diseases and immune problems for the recipient. Accordingly, replacing these environments with healthy environments, at least with human resources, is essential. One of the media that can be used as an alternative to animal serums is Wharton acellular jelly (AWJ). Therefore, in this study, we intend to replace FBS with Wharton jelly and investigate its effect on the expression of megakaryocyte-related genes and markers in stem cells. Materials and Methods: In this study, cord blood-derived CD34 positive HSCs were cultured and expanded in the presence of cytokines including SCF, TPO, and FLT3-L. Then, the culture of expanded CD34 positive HSCs was performed in two groups: 1) IMDM culture medium containing 10% FBS and 100 ng / ml thrombopoietin cytokine 2) IMDM culture medium containing 10% AWJ, 100 ng / ml thrombopoietin cytokine. Finally, CD41 expressing cells were analyzed with the flow cytometry method. The genes related to megakaryocyte lineage including FLI1 and GATA2 were also evaluated using the RT-PCR technique. Results: The expression of CD41, a specific marker of megakaryocyte lineage in culture medium containing Wharton acellular jelly was increased compared to the FBS group. Additionally, the expression of GATA2 and FLI1 genes was significantly increased related to the control group. Conclusion: This study provided evidence of differentiation of CD34 positive hematopoietic stem cells from umbilical cord blood to megakaryocytes in a culture medium containing AWJ. 相似文献
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目的:研究益气温阳法联合顺铂对Lewis肺癌小鼠调节性T(T Regulatory,Treg)细胞的影响。方法:建立40只近交系C57BL/6纯系雄性小鼠Lewis肺癌细胞皮下移植瘤模型后,将40只造模后的小鼠随机分为模型组、中药组、顺铂组及联合用药组,每组10只,单笼饲养。模型组以0.4 mL生理盐水连续灌胃12 d;中药组以0.4 mL中药液连续灌胃12 d(20 g生药/kg);顺铂组以0.2 mL顺铂溶液腹腔注射,分别在第1天、第3天、第5天腹腔注射顺铂溶液(4 mg顺铂/kg);联合用药组以0.4 mL中药液连续灌胃12 d(20 g生药/kg),在第1天、第3天、第5天腹腔注射顺铂溶液0.2 mL(4 mg顺铂/kg)。给予药物干预12 d后,用流式细胞术检测各组小鼠脾脏组织Treg细胞的比例。结果:流式细胞术结果表明,益气温阳法治疗可显著降低Lewis肺癌小鼠脾脏组织Treg细胞,与模型组比较,中药组、顺铂组和联合用药组小鼠脾脏组织内CD4+CD25+Treg细胞比例与CD4+CD25+Foxp3+Treg细胞比例均显著降低(P<0.05)。结论:益气温阳法通过降低Treg细胞的水平增强机体的抗肿瘤免疫应答而发挥抑瘤作用。 相似文献
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Julien Schaller Hlne Maby-El Hajjami Sylvie Rusakiewicz Kalliopi Ioannidou Nathalie Piazzon Alexandra Miles Dla Golshayan Olivier Gaide Daniel Hohl Daniel E. Speiser Karin Schaeuble 《Oncotarget》2021,12(7):638
Although tumor-associated lymphangiogenesis correlates with metastasis and poor prognosis in several cancers, it also supports T cell infiltration into the tumor and predicts favorable outcome to immunotherapy. The role of lymphatic vessels in skin squamous-cell carcinoma (sSCC), the second most common form of skin cancer, remains mostly unknown. Although anti-PD-1 therapy is beneficial for some patients with advanced sSCC, a greater understanding of disease mechanisms is still needed to develop better therapies.Using quantitative multiplex immunohistochemistry, we analyzed sSCC sections from 36 patients. CD8+ T cell infiltration showed great differences between patients, whereby these cells were mainly excluded from the tumor mass. Similar to our data in melanoma, sSCC with high density of lymphatic endothelial cells showed increased CD8+ T cell density in tumor areas. An entirely new observation is that sSCC with perineural infiltration but without metastasis was characterized by low lymphatic endothelial cell density. Since both, metastasis and perineural infiltration are known to affect tumor progression and patients’ prognosis, it is important to identify the molecular drivers, opening future options for therapeutic targeting. Our data suggest that the mechanisms underlying perineural infiltration may be linked with the biology of lymphatic vessels and thus stroma. 相似文献