高海拔（2261m）急性习服期大鼠脑内β—EP含量的实验研究（之三）

选用标化成年雄性Wistar大鼠、以放射免疫测定法（RIA），研究了大鼠由平原引入高海拔环境后，在24小时急性司服期，垂体及各脑区内β－内啡肪样免疫活性物质（β－EPLI）的含量变化。结果表明，大鼠引入高海拔环境后脑内β－EP含量发生明显的动态变化。高海拔实验组与平原对照组相比，垂体内β－EP含量降低非常显著（P＜0．01）。纹状体、下丘脑、丘脑、桥延、海马、皮层内β－EP含量增加非常显著（P＜0．01）。中脑内β－EP含量虽然也增加，统计学处理无显著性意义（P＞0．05）。据此推测：大鼠急进高海拔低氧环境后垂体β－EP的分泌与释放受到影响。提示，脑内β－EP参与低氧的应激反应过程，可能作为神经递质和调质，通过神经──内分泌环路在应激反应中起介导作用，β－EP可能为中枢神经系统内调节应激反应的主要应激激素之一。     

A型肉毒毒素对疱疹后神经痛患者疗效及安全性分析

目的 评价皮内注射A型肉毒毒素治疗疱疹后遗神经痛(PHN)的疗效和安全性。方法80例PHN患者,随机分为治疗组和对照组,每组40例。治疗组:皮下注射含100单位肉毒素A的生理盐水20 mL,对照组:皮下注射含有100 mg利多卡因的生理盐水20 mL。评价治疗前、治疗后1天、2周、8周疼痛症状严重程度(VAS)。采用放射免疫分析法测定β-内啡肽。结果治疗2周、8周VAS评分治疗组和对照组之间差异显著,治疗组总有效率为80%显著高于对照组的56.7%,治疗组血浆β-内啡肽含量明显升高,均无严重不良反应。结论皮内注射A型肉毒素治疗PHN安全、有效,血浆β-内啡肽可能参与了其作用机制。     

The circadian rhythm of atrial natriuretic peptide,vasoactive intestinal peptide,beta-endorphin and cortisol in healthy young and elderly subjects

Atrial natriuretic peptide, vasoactive intestinal peptide, beta-endorphin and cortisol are humoral variables characterized by a 24-h periodicity. We evaluated the circadian rhythm of these peptides and hormones in healthy subjects who were young (between 20–25 years) or elderly (between 65–75 years). All were on controlled diets. Blood samples were collected six times during a 24-h period (at 06.00, 08.00, 12.00, 18.00, 20.00 and 24.00 h) beginning 8-h after start of recumbency. The time-related data were analysed by the Cosinor method in order to validate the circadian rhythm and to quantify rhythmometric parameters which included the midline estimate of rhythm (mesor). In contrast to the young subjects, Cosinor analysis failed to reveal a significant circadian rhythm in elderly subjects, for plasma cortisol. In elderly subjects oscillation (mesor) of atrial nutriuretic peptide was higher, while that of vasoactive intestinal peptide and beta—endorphins was lower. The results suggest changes in the physiological secretion of these three peptides in healthy elderly subjects.