Use of Second Window ICG in spinal cord biopsy of a mildly contrast-enhancing lesion: Technical note and review of the literature

IntroductionIndocyanine green (ICG) is commonly used to visualize cerebral vasculature, particularly in the management of cerebral aneurysms. There have also been attempts to use ICG for visualization of tumors. Injection of ICG followed by immediate fluorescence microscopy is limited by the short time window for imaging and administration and restricted depth of imaging. Second Window Indocyanine Green (SWIG) addresses these issues by allowing for longer contrast times and the imaging of deeper regions of brain tissue. Biopsy of spinal cord lesions is often difficult for a variety of reasons, including the delicate nature of the tissue and differentiating normal from lesional tissue visually, especially in lesions with heterogeneous enhancement.MethodsIn this case report, we describe the use of second window ICG to facilitate the visualization of a spinal cord lesion and subsequent biopsy of the lesion.ResultsThis patient is a 24-year-old female who had recurrence of a suprasellar germinoma. An MRI of the rest of the neuraxis was performed to assess for the presence of drop metastases. The spinal cord from C2-5 was expanded with areas of patchy enhancement; however, this lesion was asymptomatic. The patient's oncologist requested a biopsy of this lesion to help direct subsequent care of her recurrent germinoma. The day before surgery, the patient had an intravenous injection of ICG dye. She then underwent a C3-5 laminectomy for biopsy of her cervical intramedullary lesion. After opening of the dura, no visible abnormality of the spinal cord could be identified. A Stryker endoscope showed an area of ICG uptake in the cord at approximately the C3-4 level. A midline myelotomy was centered over the ICG demarcated area and several samples were taken for pathology. Final biopsy results determined the lesion to be spinal cord parenchyma with perivascular and intraparenchymal lymphocytes – not consistent with spinal cord tumor or germinoma.ConclusionSecond Window ICG is effective in visualizing otherwise visually unremarkable spinal cord lesions. This technology can facilitate biopsy of these lesions and possibly their surgical resection.     

Porcelain laminate veneers: Clinical survey for evaluation of failure

ObjectiveTo investigate the association of the failure of porcelain laminate veneers with factors related to the patient, material, and operator.MethodsThis clinical survey involved 29 patients (19 women and 10 men) and their dentists, including undergraduate and postgraduate dental students and dental interns. Two questionnaires were distributed to collect information from participants. All patients were clinically examined. Criteria for failure of the porcelain laminate veneers included color change, cracking, fracture, and/or debonding.ResultsA total of 205 porcelain laminate veneers were evaluated. All of the restorations were fabricated from IPS e.max Press and cemented with Variolink Veneer (Ivoclar Vivadent, Schaan, Principality of Liechtenstein) or RelyX veneer cement (3M ESPE, St. Paul, MN, USA). The preparations were generally located in enamel (58.6%), and most veneers had an overlapped design (89.7%). Ten patients (34.48%) showed veneer failure, most often in terms of color change (60%). Overall, 82.8% of patients were satisfied with their restorations.ConclusionInsufficient clinical skills or operator experience resulted in restoration failure in one-third of patients.     

FKBP52在反复体外受精种植失败患者种植窗期子宫内膜的表达研究

目的探讨FKBP52在反复体外受精种植失败患者种植窗期子宫内膜的表达情况。方法采用实时荧光定量聚合酶链式反应(RT-PCR)技术和免疫组织化学方法分别测定20例反复体外受精种植失败妇女(组1)种植窗期子宫内膜组织中FKBP52蛋白及其mRNA的表达,并与15例正常生育能力妇女卵泡晚期(组2)和种植窗期(组3)相比较。结果组1的FKBP52mRNA表达水平低于组2,组2低于组3,但差异均无统计学意义(P0.05)。FKBP52蛋白表达于腺上皮的胞核及胞浆,组1在腺上皮的胞核表达明显,组2在腺上皮的胞浆表达明显,组3在腺上皮的胞核及胞浆均有明显表达;组1和组2的FKBP52蛋白表达量分别为(2 125.9±1 729.9)和(1 360.2±853.7),并显著低于组3(8 319.1±5 873.8)(P0.05,P0.01)。结论 FKBP52蛋白在种植窗期的表达和定位异常可能是反复体外受精种植失败患者子宫内膜容受性的影响因素。     

集合HIV RNA RT-PCR方法在高危人群中窗口期检测的应用

探讨使用集合艾滋病病毒(HIV)核糖核酸(RNA)反转录-聚合酶链反应(RT-PCR)的方法,对高危人群窗口期进行检测。方法采用50∶1(一级pools)、10∶1(二级pools)、1∶1(单个样本)三级集合HIV RNA,用RT-PCR方法进行样本检测。结果检测的3 904份HIV抗体阴性样本中,检出8份样本的病毒载量高于检测线。结论提示该集合方法对于大样本量的阴性样本筛查还是具有可操作性的。     

不同CT窗技术及后处理技术对肺栓塞的诊断价值

目的评价不同CT窗技术及后处理技术对肺栓塞的诊断价值。方法回顾性分析2018年9月～2020年1月在我院行CTPA确诊的45例肺栓塞患者的资料,分别应用常规纵膈窗及常用调节窗、常规纵膈窗+调节窗观察肺栓塞的位置(段以上、段及段以下)及阳性血管数,以纵膈窗联合调节窗检出的肺栓塞作为金标准。比较最大密度投影(MIP)、多平面重组(MPR)和容积重建(VR)三种后处理技术对不同位置的肺动脉显示率,组间比较采用χ~2检验。结果段及段以下肺栓塞,单独使用调节窗的漏诊率低于单独使用纵膈窗,分别为3.3%、12.5%,差异有统计学意义(P0.05)。段以上肺栓塞,单独应用常用调节窗、纵膈窗漏诊率分别为4.5%、13.6%,组间比较差异无统计学意义(P0.05)。MPR对段及段以下肺动脉栓塞的显示率优于MIP及VR影像,差异具有统计学意义(P0.05)。结论在阅读CTPA图像中,单纯使用常规纵膈窗或常用调节窗都可漏掉部分栓塞,特别是较小血管的栓塞,临床工作中,应常规使用纵膈窗+常用调节窗宽、MPR重建技术减少肺栓塞的漏诊。     

胃肠道充气螺旋CT的窗口技术

目的：探讨CT显示胃肠道及其病变的最适宜窗口。方法：随机选取50例行胃肠道充气螺旋CT检查者断面图像68帧，在四种不同窗宽窗位下，观察六个指标的显示情况。结果：第③窗（窗宽为180-280Hu，窗位为-20- 20Hu）时，直径约0.3-0.5cm细小结节或小血管断面，软组织内密度对比，脂肪与软组织对比的显示为优，气体与脂肪的对比、胃肠腔轮廓及软组织形态轮廓方面显示较好，可不同程度兼顾到所需观察的多种组织，较好显示胃肠道及其病变。结论：窗宽180-280Hu，窗位-20- 20Hu，是观察胃肠道的较适宜窗口。     

便携式心音采集与处理系统的设计

目的:在心脏辅助泵血装置的研制过程中,需要一款携带方便、抗干扰能力强的心音采集与处理系统实现对心音的准确采集,同时能够将采集到的信号进行分析。方法:本文以单片机和Visual C++6.0为基础设计了一款便携式心音采集与处理系统;该系统通过随身携带的部件采集心音信号并利用蓝牙技术无线传输到PC机,实现信号的保存和和处理。结果:该系统能实现心音信号的采集、保存、波形的实时显示、采样频率设置、心音包络提取等。结论:采用该系统采集了二尖瓣区的心音信号并提取了其包络,通过与专业设备采集的数据和处理结果对比,证明该心音采集和处理系统采集的信号准确性高、可靠性强,提取的包络效果良好。     

滑动时间窗视角下科学基金研究前沿探测

研究前沿是某领域处于先锋地位的研究内容。想要更好的理解当下和未来的研究趋势,需要时刻关注领域的研究前沿。文章综述了前人的研究方法,指出其共有问题,提出滑动时间窗视角下基于科学基金的前沿探测方法。并在选取最优聚类算法的基础上,以肿瘤学为例探测其研究前沿。结果表明Agglomerative的聚类效果最优,对4个滑动时间窗内的数据进行共词聚类分析,探测其研究前沿并分析其演化规律。