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排序方式: 共有321条查询结果,搜索用时 46 毫秒
1.
目的:观察冰蜜生肌膏促进体外培养兔成纤维细胞增殖的影响。方法:采用消化分离法进行兔成纤维细胞的原代培养,通过形态学和免疫组织化学的方法鉴定成纤维细胞。第3代纯化的成纤维细胞分为5组,空白对照组每孔加入等量的PBS,实验组每孔分别加入125、62·5、31·25、15·625mg/ml的冰蜜生肌膏提取液进行培养,培养24、48、72h后分别行MTT法检测每孔的光吸收值(OD值)。结果:冰蜜生肌膏提取液加入到成纤维细胞中培养24、48、72h后,经MTT法检测,不同浓度(浓度分别为125、62·5、31·25、15·625mg/ml)冰蜜生肌膏与对照组OD值比较差异有显著性统计学意义(P<0·05)。结论:15·625mg/ml浓度冰蜜生肌膏对成纤维细胞的生长有明显促进作用(P<0·05),药物浓度在15·625~62·5mg/ml时其作用随药物浓度升高而增加,并在浓度为62·5mg/ml时达到最大效应值(P<0·01),当药物浓度大于62·5mg/ml时则作用趋向于饱和,提示一定浓度范围内的冰蜜生肌膏药液能明显增加成纤维细胞数量,且促进作用和浓度呈显著依赖性。 相似文献
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胰岛素体外透皮特性研究 总被引:5,自引:0,他引:5
目的 考察胰岛素透皮吸收特性及透皮促进剂对其透皮行为的影响。方法 通过V-C扩散池,用紫外分光光度法测定胰岛素的透皮吸收动力特性及透皮促进剂的促进作用。结果 胰岛素经离体小鼠、家兔、大鼠和人体皮肤的渗透系数分别为16.37,17.54,8.23和9.15。而氮酮(Azone)、冰片、油酸有明显的促透作用,其中3%的Azone和2%的冰片使渗透系数分别增加了4.9和6.1倍。结论 胰岛素在大鼠皮肤中渗透系数与人体皮肤接近,冰片和Azone具有促进药物渗透作用。 相似文献
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一种新来源天然冰片与合成冰片的致突变性比较研究 总被引:4,自引:0,他引:4
目的与方法采用鼠伤寒沙门氏菌回复突变试验(Ames试验),比较天然冰片与合成冰片的致突变作用.结果在标准平板掺入法试验中,天然冰片与合成冰片在0.4~250.0μg/皿范围内,无论有或无S9活化系统,对鼠伤寒沙门氏菌突变株(TA97、TA98、TA100、TA102)的回变菌落数均无明显影响,且回变菌落背景正常.结论新来源天然冰片与合成冰片在该实验室条件下均不具致突变效应. 相似文献
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Délia Chaves Moreira dos Santos Marselle Leite Silvério de Souza Eliane Morais Teixeira Líndicy Leidicy Alves José Mário Carneiro Vilela Margareth Andrade 《Journal of drug targeting》2018,26(4):357-364
This work aimed to optimise a new nanoemulsion (NE) formulation loaded with Amphotericin B (AmB) and to evaluate its in vivo antileishmanial activity and in vitro haemolytic toxicity. The influence of gradual increases in pressure, using a high-pressure homogeniser, was evaluated. The NE was characterised for droplet size, polydispersity index, zeta potential and encapsulation efficiency (EE). For antileishmanial activity studies, AmB-NE was administered intravenously in mice infected by Leishmania infantum chagasi, which causes Visceral Leishmaniasis (VL). When the NE was submitted to gradual increases in pressure, the PI values and droplet size decreased. The droplet size (~145?nm) was lower than that obtained in previous studies. The zeta potential was negative and the EE was almost 100%. The haemolytic toxicity, evaluated on human red blood cells, for AmB-loaded NE was lower than that observed for the conventional AmB (C-AmB). C-AmB at 2?mg/kg was very toxic. In contrast, administration of the AmB-loaded NE, at same dose, did not result in any sign of acute toxicity, promoting a significant reduction in parasite burden as compared to the C-AmB. These findings suggest that this new AmB-loaded NE constitutes an attractive alternative for the treatment of VL due to improved efficacy and lower toxicity. 相似文献
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The ubiquitous gram-positive bacterium Staphylococcus aureus occupies a unique niche in humans for its ability to survive both as a commensal and a life-threatening pathogen. Its complex relationship with the host and its ability to engender a throng of virulence factors, have hindered the development of a successful vaccine against it. The use of immunoadjuvants to enhance host immunity and prevent the shift from commensalism to pathogenicity is a rational approach for containing infection. The objective of this study was to understand the mechanisms by which alum and two phytol-derived immunoadjuvants, phytanol (PHIS-01) 1 and phytanyl chloride (PCl) 2 shape the interaction between S. aureus and its murine host. We studied the effects of the phytol derivatives, relative to alum, on the induction of inflammatory cytokines and chemokines, recruitment of CD11b+ cells, generation of specific anti-S. aureus antibodies and in vitro clearance of S. aureus. Our results showed that both PHIS-01 and PCl were stronger inducers of protective cytokines IL-17 and IL-1β than alum, and far exceeded alum in enhancing anti-S. aureus antibody response. However, both alum and the phytol derivatives (particularly PCl) promoted efficient recruitment of CD11b+ cells. Furthermore, PHIS-01, alum and to a lesser extent, PCl were able to up-regulate the expression of key inflammation-related genes that were highly down-regulated by S. aureus alone. In vitro killing assays showed that both PHIS-01 and PCl were far more potent than alum in promoting S. aureus clearance; this indicated their efficiency in shaping an effective anti-S. aureus immune microenvironment. In summary, our study provides evidence for the better effectiveness of phytol-derived immunoadjuvants over alum in enhancing anti-S. aureus immunity. 相似文献
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目的:对黔产艾纳香石油醚部位的化学成分进行研究.方法:采用硅胶、凝胶等柱色谱技术对艾纳香石油醚部位的化学成分进行分离、纯化,再结合核磁共振技术鉴定其化学结构.结果:分离并鉴定了5个化合物,分别为花椒油素(1)、豆甾醇(2)、三十一烷(3)、龙脑(4)、4,22-二烯-3-酮豆甾烷(5).结论:5个化合物均首次从艾纳香石油醚部位分离得到. 相似文献