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Objective: To demonstrate the relationship between actin phosphorylation and actin sequestration in ATP-depleted rabbit renal proximal tubules. Methods: Using two-dimensional electrophoreses and Western blotting to analyze the phosphorylation state of the sequestered actin in rabbit renal proximal tubules. Results: The analytical result of the sequestered actin indicated that nearly half of the actin was phosphorylated on serine residue(s). Conclusion: Result suggested a close correlation between actin sequestration and actin phosphorylation in ATP-depleted rabbit renal proximal tubules. 相似文献
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Y Marikovsky R S Weinstein E Skutelsky D Danon 《Mechanisms of ageing and development》1985,29(3):309-316
ATP depletion crenates human red blood cells. With ferritin-avidin (FA) and cationized ferritin (CF) cell surface labeling, it is demonstrated that the discocyte----crenated shape transformation alters the two-dimensional topography of negative charge sites. With restoration of ATP levels, cell shape and charge topography return to normal. Concurrent changes in red cell shape and surface charge topography can be explained by associations between membrane integral proteins and the red cell cytoskeleton. 相似文献
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YingchunLi~ YingbinGe JunDu RongZhou JinChen LuoGu 《南京医科大学学报(英文版)》2005,19(2):72-75
Objective: To demonstrate the relationship between actin phosphorylation and actin sequestration in ATP-depleted rabbit renal proximal tubules. Methods: Using two-dimensional electrophoreses and Western blotting to analyze the phosphorylation state of the sequestered actin in rabbit renal proximal tubules. Results: The analytical result of the sequestered actin indicated that nearly half of the actin was phosphorylated on serine residue(s). Conclusion: Result suggested a close correlation between actin sequestration and actin phosphorylation in ATP-depleted rabbit renal proximal tubules. 相似文献
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