Circulating adipocyte fatty acid binding protein is increased in chronic and acute renal dysfunction

Background and aimsThe adipokine adipocyte fatty acid binding protein (AFABP) is positively associated with the development of the metabolic syndrome, diabetes mellitus, and cardiovascular disease. We hypothesized that AFABP also increases with deteriorating renal function.Methods and resultsSerum AFABP levels were quantified by enzyme linked immunosorbent assay in 532 patients with chronic kidney disease (CKD) covering the whole spectrum of estimated glomerular filtration rate (eGFR) categories from G1 to G5 (study population 1). Furthermore, AFABP was measured in 32 patients before and within 30 h after elective unilateral nephrectomy, a model of acute kidney dysfunction (AKD) (study population 2). Moreover, circulating AFABP was investigated in rats undergoing bilateral nephrectomy (BNE) as compared to sham-operated animals.Median serum AFABP levels adjusted for age, gender, and body mass index significantly increased with increasing eGFR category (G1: 22.0 μg/l; G2: 34.6 μg/l; G3: 56.7 μg/l; G4: 95.2 μg/l; and G5: 173.9 μg/l). Furthermore, renal dysfunction remained positively associated with AFABP in multivariate analysis in this cohort. In patients undergoing unilateral nephrectomy, AFABP increased significantly after surgery (42.1 μg/l) as compared to pre-surgical values (29.3 μg/l). Furthermore, relative changes of post-to-pre-surgical AFABP levels were independently associated with relative changes of post-to-pre-surgical creatinine concentrations. After BNE in rats, AFABP increased significantly as compared to sham-operated animals.ConclusionsWe show that AFABP is significantly elevated in CKD and AKD patients. Furthermore, measures of renal function are associated with circulating AFABP. Moreover, animal experiments indicate that AFABP levels strongly depend on renal function.     

C1q/肿瘤坏死因子相关蛋白9脂肪细胞型脂肪酸结合蛋白在妊娠期糖尿病患者血清中的表达及临床意义

目的探讨C1q/肿瘤坏死因子相关蛋白9(CTRP9)、脂肪细胞型脂肪酸结合蛋白(AFABP)在妊娠期糖尿病(GDM)患者血清中的表达及其临床意义。方法选取2018年4月-2019年4月期间在锦州医科大学附属第一医院进行产前检查的孕妇502例作为研究对象,根据孕24~28周的口服葡萄糖耐量试验(OGTT)结果进行分组,将结果为阳性的53例纳入GDM组,将结果为阴性的449例纳入对照组。比较GDM组、对照组的一般资料、糖脂代谢指标以及血清CTRP9、AFABP水平,分析GDM发生的危险因素,分析GDM患者血清CTRP9、AFABP与相关指标的相关性及其对GDM发生的预测价值。结果GDM组的孕前体质指数、糖尿病家族史占比、空腹血糖(FPG)、糖化血红蛋白(HbA1c)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及AFABP高于对照组,CTRP9低于对照组(P<0.05);多因素Logistic回归分析显示,孕前体质指数、FPG、HbA1c及AFABP是GDM发生的危险因素,CTRP9是保护因素(P<0.05);经Pearson分析显示,GDM患者血清CTRP9与FPG、HbA1c、TC及LDL-C均呈负相关(P<0.05),血清AFABP与孕前体质指数、FPG、HbA1c、TC均呈正相关(P<0.05);经ROC分析显示,血清CTRP9、AFABP对GDM发生均有一定的预测价值,但单独检测的预测价值不高,曲线下面积均低于0.7。二者联合检测可在一定程度上提高对GDM发生的预测价值。结论CTRP9、AFABP在GDM患者血清中呈异常表达,其表达水平与糖脂代谢水平存在一定的相关性,二者联合检测对GDM发生有一定的预测价值。     

脂肪因子nesfatin-1与妊娠期糖尿病患者AFABP、瘦素、GLP 1的相关性

【摘要】 目的 探讨脂肪因子nesfatin 1与妊娠期糖尿病（Gestational diabetes mellitus,GDM）患者脂肪细胞型脂肪酸结合蛋白（Adipocyte Fatty Acid BindingProtein ,AFABP）、瘦素、胰高血糖素样肽 1（Glucagon like peptide 1,GLP 1）的相关性。 方法 选取2015年3月～2017年6月我院妇产科收治的68例妊娠期糖尿病患者作为GDM组,选取同期50例正常糖耐量（Normal Glucose Tolerance,NGT）妊娠妇女作为NGT组。对两组患者行回顾性分析,采用SPSS对脂肪因子nesfatin 1和糖尿病患者相关指标进行pearson相关性及多元回归分析。 结果 GDM 组产前体重指数（BMI）、△BMI（产前BMI 孕前BMI）、空腹血糖、空腹胰岛素、nesfatin 1、瘦素、AFABP均显著高于NGT 组,差异有统计学意义（P＜0.05）;两组产妇孕前BMI及GLP 1差异无统计学意义（P＞0.05）;pearson相关性分析提示nesfatin 1与孕前 BMI、产前BMI 、孕期增加体重、△BMI、空腹胰岛素、AFABP相关（P＜0.05）;多元回归分析提示,产前 BMI及空腹胰岛素是nesfatin 1的独立危险因素。校正空腹胰岛素和产前 BMI 后logistic回归分析nesfatin 1、瘦素、AFABP为GDM的独立危险因素（P＜0.05）。结论 GDM患者nesfatin 1与AFABP呈正相关,nesfatin 1、AFABP、瘦素为GDM患者的独立危险因素,血清GLP 1与GDM无关。     

The brown-fat-secreted adipokine neuregulin 4 is decreased in gestational diabetes mellitus

Aims

Neuregulin 4 has recently been recognized as a novel adipokine secreted by brown adipose tissue (BAT), with beneficial effects on murine insulin resistance and hepatic steatosis. Yet, thus far, neither regulation of neuregulin 4 in gestational diabetes mellitus (GDM) nor its longitudinal changes in the peripartum period have been elucidated.