2013-2014年季节性流行性感冒疫苗免疫血清针对我国流行株的抗体水平分析

目的 分析季节性流行性感冒(流感)疫苗免疫血清针对我国流行株的抗体水平及我国流行株与疫苗株的匹配性. 方法 采集不同年龄组人群疫苗接种前后的血清,利用流感流行株和疫苗株作为病毒抗原,应用血凝抑制试验(HI)方法对血清进行检测. 结果 三价流感疫苗对我国A(H1N1)Pdm09亚型流行株产生的抗体平均几何滴度(GMT)低于针对疫苗株病毒的GMT,血清抗体滴度 40的比例为57.0%～63.3%;对我国A(H3N2)亚型流行株产生的GMT与针对疫苗株病毒的GMT类似,血清抗体滴度超过40的比例为57.6%～63.0%;对B型流行株产生的血清抗体GMT低于针对疫苗株的GMT,不过超过了疫苗株抗体GMT的50%.不同年龄组的血清抗体反应不同,成年组相对较高. 结论 2013-2014年季节性流感疫苗与同期流行的A(H1N1)Pdm09亚型流感病毒和B型流行株Yamagata系流感病毒较为匹配;H3N2亚型疫苗与流行株的匹配性较低,有可能会导致疫苗的保护效果降低.     

脐血清对人胎胰岛样细胞团增殖作用的研究

目的探讨脐血清对人胎胰岛样细胞团体外增殖和胰岛素分泌的影响,为临床胰岛细胞移植奠定基础.方法取水囊引产的胎儿胰腺,胶原酶消化法制备胰岛样细胞团,分别加入脐血清和小牛血清培养,进行形态学观察,同时放射免疫法测定胰岛素分泌量.结果脐血清组胰岛样细胞团的获得量为765±76/孔,是小牛血清组186±55/孔的4倍(P＜0.05),脐血清组总胰岛素的分泌量为1049.17±26.82ng/孔,明显高于小牛血清组的405.33±19.15 ng/孔(P＜0.05).结论脐血清能有效地促进人胎胰岛B细胞的体外增殖,显著提高胰岛样细胞团的获得量和胰岛素的分泌量.     

Serum concentration of sFas and sFasL in healthy HBsAg carriers, chronic viral hepatitis B and C patients

AIM: To estimate the amount of apoptosis among healthy HBsAg carriers, patients with chronic HBV infection treated with lamivudine and patients with chronic HCV infedJon treated with interferon alpha and ribavirin. Activity of apoptosis was evaluated by serum sFas/sFasL concentration measurement.Moreover dependence between apoptosis and HBV-DNA or HCV-RNA levels was studied.METHODS: Eighty-six persons were included into study: 34 healthy HBsAg carriers, 33 patients with chronic HBV infection and 19 patients with chronic HCV infection. Serum levels of sFas/sFasL were measured by ELISA assay. HBV-DNA and HCV-RNA were measured by RT-PCR assay. Levels of sFas/sFasL were determined before and 2 and 12 wk after therapy in patients with chronic hepatitis B and C infection.HBV-DNA or HCV-RNA was detected before treatment and 6 mo after treatment.RFSULTS: Twenty-four (71%) healthy HBsAg carriers showed HBV-DNA over 10^5/mL, which was comparable to the patients with chronic hepatitis B. Independently from HBV-DNA levels,the concentration of sFas among healthy HBsAg carriers was comparable to healthy persons. Among patients with chronic hepatitis B and C, the concentration of sFas was significantly higher in comparison to healthy HBsAg carriers and healthy persons. In chronic hepatitis B patients the concentration of sFas was decreased during lamivudine treatment. Among chronic hepatitis C patients the concentration of sFas was increased during IFN alpha and ribavirin treatment, sFasL was not detected in control group. Furthermore sFasL oo:urred more frequently in chronic hepatitis C patients in comparison to chronic hepatitis B patients.CONCLUSION: There are no correlations between apoptosis and HBV-DNA levels. However ther is an association between apoptosis and activity of inflammation in patients with chronic HBV infection. Apoptosis can be increased in patients with chronic hepatitis C by effective treatment which may be a result of apoptosis stimulation by IFN-α.     

日本血吸虫（中国大陆株）22.6kDa重组抗原的免疫原性研究

用日本血吸虫（中国大陆株）22．6kDa重组抗原（rSj22．6）免疫一批昆明小鼠，获得特异性免疫血清，其与22．6kDa重组抗原和日本血吸虫成虫抗原作免疫印迹试验（Westernblot），结果显示可特异地识别重组抗原和成虫抗原中22．6kDa分子，而正常鼠血清则不能识别。实验中，血清经去除抗大肠杆菌抗体的处理后，其与大肠杆菌的非特异性反应条带被去除，特异性反应的条带则增强。     

抗日本血吸虫未成熟虫卵部分纯化抗原免疫血清的制备及定位观察

抗日本血吸虫未成熟虫卵部分纯化抗原免疫血清的制备及定位观察汪世平周汨波曾庆仁赵慰先日本血吸虫卵不同发育时期抗原性存在明显差异。来源于不同发育阶段的虫卵可溶性抗原所诱导的宿主免疫应答明显不同。因此，制备针对未成熟虫卵可溶性抗原（ＳＩＥＡ）的免疫血清，对...     

教学用兔抗人全免疫血清的制备与效价测定

目的探讨制备高效价兔抗人免疫血清的方法。方法将人全血清经弗氏佐剂完全乳化,采用一定的免疫程序免疫家兔,末次加强免疫5d后收集兔免疫血清,利用双向免疫扩散实验、对流免疫电泳和间接酶联免疫吸附法分别测定血清中抗体的效价。结果家兔在全程免疫后产生了高效价抗体,效价分别达到了1∶16、1∶8、1∶64 000。结论用人全血清免疫家兔,可以获得高效价的抗血清,为进一步实验教学和实验研究奠定了基础。     

质控血清和患者标本数据在临床化学室内质量控制中的应用

目的 探讨质控血清联合患者数据在临床生物化学实验室室内质量控制中的应用价值。方法 质控血清采用Westgard多规则质控方法；患者数据采用差值检查法、多参数核查法等，分析临床化学常规项目室内质控的效果。结果 运用质控血清进行室内质控能够对生化分析仪的准确性和精确度起很好的监控作用；而运用患者数据进行室内质量控制时，则可及时发现如标本采集、保存、编号等因素的影响。结论 两种室内质控方法联合运用于常规临床化学，能有效避免常见的失控现象的发生，值得推广运用。     

Clinical significance of serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 in liver cirrhosis

AIM: To investigate the relationship between insulin-like growth factor-Ⅰ, -Ⅱ (IGF-Ⅰ and IGF-Ⅱ), IGF-binding protein 3(IGFBP-3) and Child-Pugh score in patients with liver cirrhosis,and to search for potential clinical markers of liver function.METHODS: Forty-four patients with advanced liver cirrhosis of viral origin were divided into 3 groups according to severity of cirrhosis (Child-Pugh score) and 38 healthy subjects served as controls. Serum levels of IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 were measured by immunoradiometric assay.RESULTS: Serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 levels were significantly lower in patients with cirrhosis than in controls,and serum concentrations of IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 were associated with the severity of liver dysfunction, and dropped sharply during the progression of liver failure. Among these 3 parameters, serum IGF-Ⅱ was the most sensitive and effective indicator for liver dysfunction. Concentrations of IGF-Ⅰ <30 ng/mL, IGF-Ⅱ <200 ng/mL and IGFBP-3 <6 ng/mL implied a negative prognosis for patients with liver cirrhosis.CONCLUSION: Serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 may provide a new dimension in the assessment of liver dysfunction.Combined detection of serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 with Child-Pugh score is more effective in predicting prognosis than Child-Pugh score alone.     

Dot-ELISA检测广州管圆线虫抗体的研究

目的　研究Dot-ELISA诊断广州管圆线虫病的诊断价值。方法　用Dot-ELISA法检测广州管圆线虫感染大鼠血清抗体 ,并观察曼氏迭宫绦虫裂头蚴抗原免疫大鼠血清、猪蛔虫抗原免疫大鼠血清、日本血吸虫感染大鼠血清等 3种异源性血清有无交叉反应。结果　广州管圆线虫感染大鼠血清阳性率为 10 0 % ,猪蛔虫抗原免疫大鼠血清为 10 % ,其余检测血清均为阴性。结论　Dot -ELISA法用于检测广州管圆线虫病的诊断具有敏感性高 ,特异性强 ,并有简便快速等优点。