Familial Wolff-Parkinson-White syndrome is linked to the locion chromosome 7q3

Objective Wolff-Parkinson-White syndrome (WPW) is considered to be an autosomal dominanthereditary disease, but the gene is not identified. The objective of this study was to localize the genetic loci of Wolff-Parkinson-White syndrome. Methods Linkage analysis between the disease of Wolff-Parkinson-White syndrome and 3 STR (short tandem repeats) markers on 7q3 (D7S505, D7S688, and D7S483) was tested in 3 kindreds of the Wolff-Parkinson-White syndrome (101 numbers in total) by genotyping. Results Wolff-Parkinson-White syndrome was linked to the loci above. The maximum two-point Lod score detected at D7S505 was 6. 4 at a recombination fraction (θ) of 0. 1; the Lod score of D7S688, D7S483 was 5. 3 vs 2. 5. Conclusion The gene of Wolff-Parkinson-White syndrome is located at 7q3.     

金黄色葡萄球菌富丝氨酸重复蛋白SraPL⁃Lectin特异性抗体的制备及其功能分析

目的：制备金黄色葡萄球菌富丝氨酸重复蛋白SraPL?Lectin单克隆抗体,并分析其体外功能。方法：以USA300基因组为模板,PCR扩增SraPL?Lectin基因序列,克隆到pET28a表达载体中,0.1 mmol/L IPTG 25 ℃诱导6 h,镍柱亲和层析纯化SraPL?Lectin?His蛋白。以纯化的SraPL?Lectin?His重组蛋白为抗原免疫Balb/c小鼠,采用常规融合技术制备杂交瘤细胞,间接ELISA法、Western blot法筛选和鉴定阳性杂交瘤细胞株。扩大培养阳性细胞株后注射小鼠腹腔,收集腹水,经Protein G柱纯化anti?SraPL?Lectin单克隆抗体。以不同终浓度的单克隆抗体预先孵育A549细胞和注射小鼠腹腔,涂板计数单克隆抗体阻断细菌黏附、侵入和感染的能力。结果：成功构建了SraPL?Lectin?pET28a重组表达载体,经镍柱纯化后获得高纯度的重组蛋白,获得稳定分泌单克隆抗体的细胞株,小鼠腹水经Protein G柱纯化后获得纯度高达95%以上的特异性抗体,效价1∶32万。终浓度100 ng/mL单克隆抗体预先孵育A549细胞2 h,能够显著降低金黄色葡萄球菌对A549细胞的黏附和侵入。提前1 d向小鼠腹腔注射1 μg anti?SraPL?Lectin单克隆抗体,能够显著降低小鼠血液中的金黄色葡萄球菌数量。结论：本研究制备的anti?SraPL?Lectin单克隆抗体能够阻断金黄色葡萄球菌对宿主细胞的黏附和侵入,为将SraPL?Lectin作为防控金黄色葡萄球菌感染药物靶点的研发奠定了基础。