ULTRASTRUCTURAL INVESTIGATION OF EXPERIMENTAL FRACTURE HEALING ROLE OF OSTEOGENESIS PLAYED BY FIBROBLASTS

Transmission electron microscopic investigation of standardized fractures of radii in 50 rabbits re vealed that fibroblasts took part in the formation of bony callus. The osteogenetic role played by the fibroblasts can be categorized into the following 5 aspects: a. Fibroblasts synthesize and secrete Type collagen fibrils and induce deposition of calcium salt crystals in the collagen fibrils. b. Fibroblasts produce matrix vesicles in their surroundings. These matrix vesicles become calcified and turn into floccu- lent calcospherules which coalesce and fuse into bone tissues. c. Fibroblasts harbor calcium granules in their mitochondria, thus providing calcium for calci- fication of the intercellular matrix and bone forma tion between the cells. d. Fibroblasts can transform directly into osteocytes; there is bone formation around the fibroblasts, the bone tissues surround the fibroblast in the form of bony lacuna, then the fibro- blast in the lacuna transforms into osteocyte. e. Fibroblasts can undergo degenerative changes leading to decease and replacement by bone tissues.     

Ultrastructural investigation of calcification and ossification in experimental fracture healing with special reference to osteogenic role of fibroblasts.

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自体吞噬与类固醇激素分泌调节的关系

本实验用腹腔内给予下丘脑激素或类固醇激素的方法，造成大鼠睾丸间质细胞和肾上腺皮质束状带细胞处于分泌兴奋或抑制状态，对这两种分泌类固醇激素细胞中的溶酶体和自噬小体进行了超微结构、细胞化学和形态计量研究。结果表明，在类固醇激素分泌增多时，细胞中自体吞噬活动减弱；激素分泌减少时，自体吞噬活动加强。这一结果证明了细胞内的自体吞噬活动与类固醇激素的分泌调节有密切关系，自体吞噬是溶酶体参与激素分泌调节过程的一种重要方式。     

IMMUNOLOGICAL STUDIES ON THROMBOANGIITIS OBLITERANS

Using humoral immunity (gamma-globulin 18 cases, IgG, IgA, IgM each 30 cases, CH50, C3, C4 each 29 cases and CIC 31 cases), cellular immunity (E-RFC 68 cases, As 30 cases, SmIg, SmIgG each 18 cases, IMIT 30 cases) and immunopathological manifestations (light microscope, fluorescence microscope, transmitted electron microscope each 7 cases) as indices, we studied the immunological changes of "progressing", "remittent" and "stabilized" groups of patients with thromboangiitis obliterans (TAO) in different stages. Humoral immunity indicated that gamma-globulin, immune complex and IgG were all increased; cellular immunity indicated that the rate of T cells and suppressor cells was declined, while that of B cells was elevated. Immunopathologically, under light microscope all the layers of involved vessels were infiltrated with neutrophils, lymphocytes and monocytes; under fluorescence and electron microscope, immune complexes were found in the involved vessel walls. Our preliminary results suggest that TAO is an autoimmune disease relevant to antigen-antibody complex.     

溶酶体在实验性心肌肥大过程中的作用

本实验用大鼠主动脉结扎的心肌肥大模型,结合电镜酶细胞化学技术,观察心肌肥大发展过程中溶酶体的变化。结果表明:心肌肥大发展时,溶酶体参与心肌细胞改建;到心肌肥大后期,出现大量自噬现象,造成心肌不可逆损伤。     

COEXISTENCE OF OSTEOARTHRITIS AND OSTEOPOROSIS INFEMORAL HEAD: A SCANNING ELECTRONMICROSCOPIC STUDY

ffeSUtn6 Objectif Detenniner ie cusistence d' ostdoarthrite et d' ostdope dans la tste du femur. liChen 12 femmes (age moron 56 ans )atteintes d' ortdoorthrite de la hanche, on a Prdlevd la tste du femur durant la substitutiontotale de l' articulation de leur hanche. APrds das traitements, Ies tstes du femur furent studioes sons ie microscope dlectroniqued belang (soon ). ~flats to lesions ostdcorthritiques de differents degrds (azractdrithe per ba digs~nce du cartilage articulaire et d' hyp…     

AS_2O_3对消化道肿瘤细胞凋亡作用的研究

目的研究AS3O3对10种消化道肿瘤细胞株的致凋亡作用。方法采用形态学观察、流式细胞仪检测及DNA电泳分析。结果发现两株细胞经AS2O3作用后有明显凋亡,三株细胞无凋亡,其余有少量凋亡。结论AS2O3对不同的消化道肿瘤细胞有不同的作用,提示AS2O3对不同细胞的致凋亡作用可能存在不同的机理。     

食管癌、胃癌细胞对氧化砷促凋亡的敏感性与固有活性氧水平有关

目的 探讨食管癌，胃癌细胞的固有活性氧（reactive oxygen species,ROS)水平与细胞对三氧化二砷（As2O3)促凋亡敏感性之间的关系。方法 用小剂量（2μmol/L)As2O3作用于人胃癌细胞和食管癌细胞株各一对（SGC7901、MKN45和EC/CUHK1、EC1867），证实As2O3促凋亡敏感性在SGC7901和MKN45之间及EC/CUHK1和EC1867之间均有差异。然后在不加As2O3的情况下，用活性氧捕获剂双氢罗丹明123（DHR123）卵育细胞（DHR123在细胞内被ROS氧化为发出荧光的罗丹明123），通过流式细胞仪检测细胞内罗丹明123的荧光而测得细胞内固有ROS水平。结果 对As2O3敏感的SGC7901细胞的固有活性氧水平比As2O3不敏感的MKN45细胞高，对As2O3敏感的EC/CUHK1细胞的固有活性氧水平比As2O3不敏感的EC1867细胞高。结论 食管癌，胃癌细胞固有ROS水平的差异与细胞对As2O3诱导凋亡的敏感性差异有关。     

张力－应力下再生骨的超微结构研究

应用自制改良式Ｉｌｉｚａｒｏｖ外固定架对３０只山羊施行左胫骨骨干延长术。术后第７，１４，２１，２８，３５，４９和６３天分别各处死４只山羊。标本行光镜、电镜观察，发现张力－应力下骨延长骨发生符合膜内成骨，矿化沿定向胶原束逐渐形成板层骨，５～６周后成骨细胞出现，同时骨小梁直径增粗。愈合早期大多数成纤维细胞直接演变为骨细胞，在骨细胞的四周同时形成骨隐窝，使其发生可以不完全依赖于骨外膜及骨内膜的成骨细胞或骨生成细胞，能更早地在延长上、下端形成骨组织。说明成纤维细胞不仅产生胶原，而且在Ｉｌｉｚａｒｏｖ再生骨发生中起重要的作用。