首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   262篇
  免费   0篇
  国内免费   9篇
基础医学   6篇
临床医学   29篇
内科学   8篇
皮肤病学   1篇
神经病学   3篇
特种医学   6篇
外科学   86篇
综合类   62篇
预防医学   11篇
药学   41篇
中国医学   2篇
肿瘤学   16篇
  2023年   1篇
  2021年   3篇
  2020年   2篇
  2019年   3篇
  2018年   1篇
  2017年   2篇
  2016年   1篇
  2015年   9篇
  2014年   2篇
  2013年   16篇
  2012年   16篇
  2011年   17篇
  2010年   38篇
  2009年   42篇
  2008年   28篇
  2007年   24篇
  2006年   16篇
  2005年   12篇
  2004年   9篇
  2003年   10篇
  2002年   2篇
  2001年   3篇
  2000年   3篇
  1999年   4篇
  1998年   3篇
  1997年   1篇
  1996年   2篇
  1995年   1篇
排序方式: 共有271条查询结果,搜索用时 15 毫秒
1.
通讯员是医院宣传工作不可缺少的中坚力量,做好医院新闻宣传工作离不开通讯员的支持,加强通讯员队伍建设,是提升医院新闻宣传工作水平的一项重要措施.  相似文献   
2.
目的 探讨前列腺液半乳凝素3(Gal-3)浓度对诊断前列腺癌的临床价值.方法 采用人Gal-3抗体免疫组化法对31例中老年男性前列腺穿刺组织进行染色,并按病理诊断分为前列腺癌组(A组,12例)和前列腺增生组(B组,19例).应用多克隆抗体ELISA法检测前列腺液Gal-3浓度,分析两组Gal-3的表达差异.结果 A组前列腺液Gal-3浓度3703.3 μg/ml,高于B组的1028.4μ.g/ml(P<0.01);A组前列腺组织Gal-3表达量亦大于B组(P<0.01).4例前列腺癌患者合并转移性骨损害;其前列腺液Gal-3浓度高于8例非转移前列腺癌患者[(5265.6±345.2) μg/ml vs.(2292.1±1325.4) μg/ml](P<0.05).取前列腺液Gal-3浓度为4167 μg/ml时,其诊断前列腺癌的敏感度为100%,特异度为80%.结论 前列腺液Gal-3检测可用于前列腺癌的诊断和是否发生骨转移的评估.  相似文献   
3.
4.
5.
目的 探讨输尿管结石多次体外震波碎石治疗无效的原因.方法 输尿管结石经多次震波后无效患者121例,观察其治疗全过程,分析影响疗效的因素.结果 121例经多次震波碎石后只有少量碎石或无碎石排出,经后续泌尿外科腔镜或开放手术治疗,术中发现结石嵌顿息肉包裹89例,结石远端梗阻扭曲23例.结石成分分析显示为硬度较高的草酸钙、磷酸钙76例.结论 多次震波无效的原因有:结石嵌顿息肉、结石较大及较多、远端梗阻、对焦困难、结石密度高等,同时存在2个以上因素者尤其明显,临床上这类患者宜早期采用其他治疗方法.  相似文献   
6.
目的 探讨外周血中性粒细胞与淋巴细胞比值(NLR)、血小板与淋巴细胞比值(PLR)和前列腺特异性抗原(PSA)对前列腺癌的诊断价值.方法 检测119例前列腺癌患者(前列腺癌组)和97例非前列腺癌患者(非前列腺癌组)外周血中性粒细胞、淋巴细胞、血小板和PSA水平,计算NLR和PLR,分析NLR、PLR和PSA对前列腺癌的诊断效能.结果 前列腺癌组NLR、PLR和PSA高于非前列腺癌组(P<0.05).取NLR、PLR、PSA最佳临界值为2.04、128.14、8.80 ng/mL时,其诊断前列腺癌的灵敏度和特异度分别为62.7%和56.7%、64.5%和61.1%、70.9%和77.8%,AUC分别为0.604[95%CI(0.526~0.683)]、0.646[95%CI(0.570~0.722)],0.784[95%CI(0.722~0.846)].联合应用NLR、PLR和PSA诊断前列腺癌的AUC为0.830[95%CI(0.775~0.885)],PLR和PSA联合诊断的AUC为0.828[95%CI(0.772~0.883)],均大于其他单一指标诊断的AUC(P<0.05).PLR和PSA联合诊断前列腺癌的特异度最高,为91.3%,高于其他指标诊断的特异度(P<0.05).结论 联合应用外周血PLR和PSA诊断前列腺癌的特异度较单一指标更高,可作为前列腺癌的预测指标.  相似文献   
7.
目的 探讨螺旋CT平扫测量肾实质体积和厚度判断梗阻侧肾小球滤过率(glomerular filtration rate,GFR)的价值,并比较2种肾实质形态学指标与梗阻侧GFR之间的关系.方法 利用螺旋CT平扫测量71例慢性单侧上尿路梗阻性疾病患者的肾实质体积和厚度.①肾实质体积测量:扫描双肾,逐层标示各横断面面积,结合扫描层厚计算肾实质体积,并计算梗阻侧肾实质体积占总的肾实质体积百分比(%CTvol).②肾实质厚度测量:选择包含肾脏集合系统的第一个和最后一个层面的内、外、后3个位置测量肾实质厚度,取平均值作为肾实质厚度,并计算梗阻侧肾实质厚度占总的肾实质厚度百分比(%CTt).采用99Tcm-DTPA肾动态显像技术,应用Gates法测定分肾及总的GFR值,计算梗阻侧肾GFR的百分比(%GFR),采用Pearson相关分析行%CTvol、%CTt与%GFR之间的相关分析.结果 71例患者%CTvol、%CTt与%GFR之间均有明显的相关性,r值分别为0.80(t=11.20,P<0.05)和0.66(t=7.24,P<0.05),直线回归方程分别为%GFR=0.05+0.80×%CTvol(F=125.48,P<0.05)和%GFR=0.12+0.66×%CTt(F=52.36,P<0.05).结论螺旋CT平扫测量肾实质体积和厚度可以作为临床上评价慢性梗阻侧肾GFR的方法,肾实质体积较肾实质厚度更准确.
Abstract:
Objective To evaluate the value of renal parenchymal volume and thickness by non-contrast spiral CT in evaluating the differential glomerular filtration rate (GFR) for chronic obstructed kidneys, and to compare the correlations between the two morphologic indices of renal parenchyma and the GFR for chronic obstructed kidneys. Methods Seventy-one patients who had a diagnosis of unilateral chronic upper urinary tract obstruction were included in this analysis. (1) The renal parenchymal volume was mea-sured by non-contrast spiral CT. Both kidneys were scanned by non-contrast spiral CT. The renal parenchymal area of each section was marked manually. Renal parenchymal volume was calculated as the sum of renal parenchymal area multiplied by the width of each section. The volume percentage of obstructed kidney (%CTvol) was also calculated. (2) Renal parenchymal thickness was measured on the first and last non-contrast CT image levels from the anterior, posterior and lateral locations of the kidney that clearly contained the collecting system. The mean of these measurements was defined as the renal parenchymal thickness. The differential renal parenchymal thickness of the obstructed kidney (%CTt) was defined as the percentage of the obstructed renal parenchymal thickness to the total renal parenchymal thickness for both kidneys. GFR was determined with 99Tcm-DTPA dynamic imaging system by Gates method. The differential GFR for obstructed kidney (%GFR) was the GFR percentage of obstructed kidney to the total GFR for both kidneys. The Pearson relation test was carried out between the %CTvol, %CTt and the %GFR respectively. Results %CTvol and %CTt correlated well with %GFR in chronic obstructed kidneys among the 71 test group patients. Pearson correlation coefficient r was 0.80 (t=11.20, P<0.05) and 0.66 (t=7.24, P<0.05), respectively. The linear correlation equation respectively was %GFR=0.05+0.80×%CTvol (F=125.48, P<0.05) and %GFR=0.12+0.66×%CTt (F=52.36, P<0.05). Conclusions Renal parenchymal volume and thickness by non-contrast spiral CT might be used as clinical practical parameters to evaluate the differential GFR for chronic obstructed kidneys. Renal parenchymal volume is more accurate than renal parenchymal thickness.  相似文献   
8.
目的 探讨前列腺液(EPS)中B7-H3分子对血清t-PSA灰区(4~10 ng/ml)内炎性PSA升高患者的鉴别诊断价值.方法 选择2009年12月至2010年4月收治的全部慢性前列腺炎(CP)患者和t-PSA灰区内行前列腺穿刺活检患者共116例,年龄19~80岁,平均40岁.CP 91例,年龄19~49岁,平均31岁.其中慢性细菌性前列腺炎(II型)11例、慢性炎症性非细菌前列腺炎(IIIA型)26例、慢性非炎症性非细菌前列腺炎(IIIB型)54例.t-PSA灰区内接受经直肠超声引导下前列腺穿刺活检患者25例,年龄62~80岁,平均71岁,t-PSA(7.21±2.60)ng/ml.其中穿刺病理结果阳性5例,Gleason评分6分2例、7分2例、8分1例;阴性20例,其中伴炎症细胞浸润11例.采用经直肠按摩法提取EPS.酶联免疫吸附法检测各组EPS B7-H3水平.健康男性对照11例,年龄24~46岁,平均30岁.既往无泌尿系不适症状及手术史.结果 对照组、II型组、IIIA型组、IIIB型组EPS中B7-H3水平依次为(49.81±11.54)、(19.33±13.90)、(17.67±15.76)、(25.14±13.44)ng/ml,穿刺阳性组、阴性不伴炎症组、阴性伴炎症组分别为(26.30±16.32)、(30.23±18.42)、(10.11±5.42)ng/ml.CP各组EPS B7-H3水平均低于对照组,差异有统计学意义(P<0.01).II型组和IIIA型组间差异无统计学意义(P>0.05),但均显著低于IIIB型组,差异有统计学意义(P<0.05).穿刺阴性伴炎症组EPS中B7-H3水平与II型组、IIIA型组比较差异无统计学意义(P>0.05),但显著低于穿刺阳性组及阴性不伴炎症组,差异有统计学意义(P<0.05).EPS B7-H3表达检测在t-PSA灰区内诊断炎性PSA升高患者的ROC曲线下面积为0.883(P=0.001),当EPS B7-H3值≤16.24 ng/ml时,诊断敏感性为78.6%,特异性为81.8%.结论 EPS B7-H3表达检测可能成为t-PSA灰区内鉴别诊断炎性PSA升高的新指标,从而减少不必要的前列腺穿刺活检.
Abstract:
Objective To investigate the value of B7-H3 in expressed prostatic secretions (EPS) in differential diagnosis of patients with inflammatory elevation of PSA in t-PSA gray zone (4-10 ng/ml). Methods One hundred and sixteen patients from the ages of 19 to 80 years (mean, 40 years) were stu-died. In the group there were 91 chronic prostatitis (CP) patients (mean age 31 years, 19-49 years), including 11 chronic bacterial prostatitis (type II) patients, 26 inflammatory nonbacterial prostatitis (IIIA) patients and 54 noninflammatory nonbacterial prostatitis (IIIB) patients. Transrectal ultrsound guided prostate biopsy was performed on 25 patients (mean age 71 years, 62-80 years) with t-PSA in gray zone (7.21±2.60 ng/ml). Five had positive results, Gleason score was 6 in two cases, 7 in two cases and 8 in one case. Twenty patients had negative results, of whom 11 patients had inflammatory cell infiltration. EPS was collected by transrectal massage, and Enzyme-linked immunosorbent assays (ELISA) were performed for B7-H3 detection. In addition, 11 normal male controls with a mean age of 30 years (24-46 years) were recruited into the study. Volunteers were excluded if they had a history of genitourinary symptoms or surgery.Results The EPS B7-H3 levels of controls, II, IIIA, IIIB groups were 49.81±11.54, 19.33±13.90, 17.67±15.76, 25.14±13.44 ng/ml, respectively. The levels of EPS B7-H3 in positive biopsy, noninflammatory negative biopsy and inflammatory negative biopsy groups were 26.30±16.32, 30.23±18.42, 10.11±5.42 ng/ml, respectively. The highest levels were found in the control group (P<0.01). Compared to the IIIB, B7-H3 levels in II and IIIA groups were significantly lower (P<0.05). There was no significantly difference between II and IIIA groups (P>0.05). The EPS B7-H3 levels in the inflammatory negative biopsy group were statistically lower than in positive biopsy and noninflammatory biopsy groups (P<0.05). But no significant differences were found among inflammatory negative biopsy, II and IIIA groups (P>0.05). Receiver operating curve (AUC=0.883, P=0.001) utilizing EPS B7-H3 levels≤16.24 ng/ml identified patients with inflammatory elevation of PSA with a sensitivity of 78.6% and a specificity of 81.8% from patients with t-PSA in gray zone. Conclusion The EPS B7-H3 detection provides a new way for differential diagnosis of patients with inflammatory elevation of PSA in t-PSA gray zone resulting in a reduction of unnecessary prostate biopsy.  相似文献   
9.
阴茎折断14例报告   总被引:1,自引:0,他引:1  
目的 探讨阴茎折断的诊治方法.方法 回顾性分析14例阴茎折断患者的临床资料.结果 14例临床表现均较典型,依据病史与体检确诊.其中8例结合彩色多普勒超声检查确诊,均发现海绵体破裂口.14例均行急诊手术治愈,术后随访6月~5年,均在术后1~3月恢复正常性生活,无阴茎畸形、海绵体硬结,无勃起疼痛及不坚.结论 典型病史和体征是阴茎折断的主要诊断依据,彩色多普勒超声检查有发现海绵体损伤和血肿部位、及指导手术切口部位的价值.急诊手术治疗可以减少并发症的发生.  相似文献   
10.
目的 探讨IKK2dn基因转染并负载供者抗原的受者未成熟树突状细胞(imDC)延长同种异体肾移植大鼠的存活时间及其机制.方法 获取和培养Lewis大鼠骨髓源性DC,转染IKK2dn并负载BN大鼠可溶性抗原进行体外实验,检测CD86和主要组织相容性复合物(MHC)Ⅱ的表达及DC刺激T淋巴细胞增殖的能力.肾移植受者为Lewis大鼠,用随即数字表法分DC组、空转染组、转染组、对照组,术前7d分别输注1×10~7个D、Adv-0-DC、负载BN抗原的Adv-IKK2dn-DC和等量生理盐水,供者均为BN大鼠.另设第三方供者组,术前处理同转染组,供者为Wistar大鼠.移植后检测各组受者T淋巴细胞的增殖能力及血清白细胞介素2(IL-2)和γ干扰素(IFN-γ)的表达水平,观察各组大鼠的存活时间和发生排斥反应情况.结果 DC的体外实验结果显示:与转染IKK2dn前相比,转染后DC仍能低水平表达CD86和MHC Ⅱ,负载供者抗原后CD86和MHCⅡ表达均增加,而转染IKK2dn后再负载供者抗原,CD86和MHC Ⅱ的表达未发生明显变化;DC负载供者抗原后,刺激T淋巴细胞增殖的能力明显增强(P<0.05),而转染IKK2dn并负载供者抗原后不能有效刺激T淋巴细胞增殖.肾移植术后的检测结果显示:转染组T淋巴细胞的增殖能力明显低于其他4组(P<0.05或P相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号