Vitamin B6 Addiction in Acute Myeloid Leukemia

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CA153联合CA199对良恶性腹腔积液的鉴别诊断价值

目的探讨腹腔积液中CA153联合CA199对良恶性腹腔积液的鉴别诊断价值。方法选择98例腹腔积液患者,经手术病理诊断为恶性腹腔积液者为观察组(48例),良性腹腔积液者为对照组(50例)。检测并比较2组患者腹腔积液中的CA153、CA199水平及CA153、CA199的阳性结果,分析CA153及CA155单用及联合应用对恶性腹腔积液的诊断效能。结果观察组腹腔积液中的CA153及CA199水平均明显高于对照组,P <0. 05。观察组CA153及CA199阳性率明显高于对照组,P <0. 05。CA153、CA199对恶性腹腔积液诊断的敏感性、特异性及准确性对比,差异无统计学意义(P> 0. 05);CA153+CA199的敏感性、特异性及准确性明显高于单项CA153及CA199(P <0. 05)。结论 CA153与CA199单项检测对恶性腹腔积液的诊断价值有限,CA153+CA199对恶性腹腔积液的诊断价值较高,值得临床推广应用。     

miR-199-3p通过对FGF2的调控抑制肝癌细胞MHCC97H的增殖、迁移作用及机制

目的:探讨miR-199-3p通过靶向调控抑制靶基因FGF2从而抑制肝癌MHCC97H细胞的增殖和迁移及机制。方法:qRT-PCR检测癌旁正常组织及肝癌肿瘤组织中miR-199-3p的表达水平；Transwell细胞侵袭实验检测肝癌细胞株Huh7、MHCC-97L、SMMC7721、HepG2和MHCC97H株其侵袭能力；qRT-PCR检测miR-199-3p在5株肝癌细胞系中的表达量；通过生物信息软件预测靶基因FGF2 mRNA 3' UTR的碱基存在miR-199-3p可能互补结合的位点并用荧光报告基因法及WB进行验证；通过qRT-PCR、免疫组化及Western blot检测FGF2在肝癌肿瘤组织和癌旁正常组织及各类肝癌细胞株表达的影响；Transwell、划痕、CCK8及流式细胞法检测miR-199-3p与FGF2对肝癌MHCC97H细胞增殖、侵袭、迁移及周期S期聚集能力的调控；采用MHCC97H细胞构建肝癌肿瘤异种移植小鼠模型进行肿瘤观测及免疫组化实验验证miR-199-3p对肝癌的调控作用。结果:miR-199-3p的表达水平与肝癌细胞侵袭转移能力相关；miR-199-3p能够通过抑制FGF2的mRNA翻译，抑制FGF2蛋白水平表达；FGF2与肝癌细胞侵袭转移能力相关；miR-199-3p可以负调控FGF2抑制肝癌MHCC97H细胞的生物行为；miR-199-3p 可抑制肝癌细胞中阳性信号，细胞增殖水平显著降低。结论:miR-199-3p通过抑制FGF2抑制肝癌细胞MHCC97H的增殖和迁移。     

Determination of Clinical Cut-Off Values for Serum Cystatin C Levels to Predict Ischemic Stroke Risk

Background: The association between cystatin C and risk of ischemic stroke is inconsistent and the cut-off values of cystatin C are diverse in different articles. We aimed to investigate the association between cystatin C levels and the development of ischemic stroke and to explore the clinical cut-off values of serum cystatin C levels for ischemic stroke. Methods: This prospective cohort study included 7658 participants from the China Health and Retirement Longitudinal Study who were free of cardiovascular diseases and cancer at baseline. A decision-tree model was used to find reasonable cut-off values for cystatin C levels. Logistic regression models were used to analyze the association between different levels of cystatin C and the risk of ischemic stroke. Results: The whole cohort was divided into the following 3 groups according to the decision tree: group-low (<.901 mg/L), group-moderate (.901～1.235 mg/L), and group-high (>1.235 mg/L). After 4 years of follow-up, we identified 156 cases of ischemic stroke. After adjusting for potential confounding factors, the odds ratios (95% confidence intervals) of ischemic stroke were 1.637 (1.048-2.556) for group-moderate and 2.326 (1.285-4.210) for group-high) compared with the low group of cystatin C. Subgroup analyses showed that the association between cystatin C levels and the incidence of ischemic stroke was more pronounced in males or old people than in females or young people. Conclusions: We found 2 suitable cut-off values for serum cystatin C levels and found that high levels of cystatin C were associated with an increased risk of ischemic stroke.     

扶正抑癌汤联合腹腔镜辅助小切口根治手术治疗胃癌临床研究

目的:探讨扶正抑癌汤联合腹腔镜辅助小切口根治手术治疗胃癌的效果及对患者免疫功能的影响。方法:选择80例胃癌患者,按随机数字表法分为观察组和对照组各40例。对照组行腹腔镜辅助小切口根治手术治疗,观察组予以扶正抑癌汤联合腹腔镜辅助小切口根治术手术治疗,比较2组患者临床疗效及免疫功能。结果:治疗前,2组中医症状评分比较,差异无统计学意义(P>0.05)。治疗后,2组中医症状评分较治疗前下降(P<0.05),且观察组中医症状评分低于对照组(P<0.05)。治疗前,2组癌胚抗原(CEA)、糖类抗原199 (CA199)、糖类抗原125 (CA125)水平比较,差异无统计学意义(P>0.05)。治疗后,2组CEA、CA199、CA125水平较治疗前下降(P<0.05),且观察组CEA、CA199、CA125水平低于对照组(P<0.05)。治疗前,2组CD4^+、CD8^+、CD4^+/CD8^+水平比较,差异无统计学意义(P>0.05)。治疗后,2组CD4^+、CD8^+、CD4^+/CD8^+水平较治疗前升高(P<0.05),且观察组CD4^+、CD8^+、CD4^+/CD8^+水平高于对照组(P<0.05)。结论:扶正抑癌汤联合腹腔镜辅助小切口根治手术治疗胃癌效果显著,可有效降低相关肿瘤标志物水平,并改善患者免疫功能。     

血清肿瘤标志物联合检测在原发性肝癌诊断中的价值研究

目的:探讨细胞周期素A2(CCNA2)与甲胎蛋白(AFP)、癌胚抗原(CEA)、癌抗原199(CA199)及癌抗原724(CA724)的联合检测在原发性肝癌临床诊断中的价值,以期筛选出理想的肿瘤标志物组合,提高肝癌诊断的准确率。方法:选取2020年10月—2021年3月天津市第三中心医院收治的原发性肝癌患者68例为研究对象,作为原发性肝癌组;另选择同期收治的80例肝病患者作为肝良性疾病组,80例健康体检者作为健康对照组。采集各研究对象清晨空腹静脉血5 mL,采用addicare1100酶免仪利用酶联免疫吸附法(ELISA)检测血清CCNA2,采用罗氏e801全自动电化学发光仪及其配套试剂检测血清AFP、CEA、CA199和CA724。评估上述指标单项及联合检测在早期PLC诊断的应用价值。结果:健康对照组血清肿瘤标志物均显著低于肝良性疾病组与原发性肝癌组,差异有统计学意义(P＜0.05)。68例原发性肝癌患者中,对AFP、CEA、CA199、CA724和CCNA2等血清肿瘤标志物水平予以检测,联合检测的特异性和准确性均显著高于各指标的单项检测结果,差异有统计学意义(P＜0.05)。结论: CCNA2作为一种新型的肿瘤标志物,对肝癌的诊断具有一定的价值。CA199、CEA、AFP、CA724、CCNA2联合检测能显著提升原发性肝癌的诊断阳性率,对尽早诊断原发性肝癌具有重要意义。     

姜黄素调控miR-199a-3p基因抑制前列腺癌细胞增殖、迁移和侵袭的研究

目的探讨姜黄素调控miR-199a-3p的基因表达对前列腺癌C4-2细胞增殖、迁移和侵袭的影响。方法将miR-199a-3p抑制物及阴性对照转染至C4-2细胞中,并分别标记为anti-miR-199a-3p组和anti-miR-con组;将仅加入脂质体的C4-2细胞标记为对照组。运用MTT法检测通过姜黄素(0、20、40、80μmol/L)处理的C4-2细胞的增殖情况。40μmol/L姜黄素处理C4-2细胞后,Transwell法检测细胞的迁移和侵袭能力;qRT-PCR检测细胞的miR-199a-3p表达量。将inhibitor NC、miR-199a-3p inhibitor转染至C4-2细胞中,再用40μmol/L姜黄素处理48 h,采用MTT法、Transwell法检测各组细胞的增殖、迁移和侵袭情况;Western blot检测各组C4-2细胞中基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)、β-catenin、Cyclin D1和c-Myc的蛋白表达量。结果与对照组比较,姜黄素能明显抑制C4-2细胞的增殖、迁移和侵袭(P<0.05)。姜黄素(40μmol/L)处理后,C4-2细胞中miR-199a-3p的表达量显著增加(P<0.05)。抑制miR-199a-3p的表达,可逆转姜黄素对C4-2细胞增殖、迁移和侵袭的抑制作用(P<0.05)。使用姜黄素处理miR-199a-3p低表达的C4-2细胞后,与anti-miR-con组相比,anti-miR-199a-3p组C4-2细胞的MMP-2、MMP-9的表达量显著增加(P<0.05),β-catenin、Cyclin D1和c-Myc蛋白表达量均显著增加(P<0.05)。结论姜黄素可上调miR-199a-3p的表达,从而抑制前列腺癌C4-2细胞的增殖、迁移和侵袭。     

HBX在肝癌细胞中通过下调miR-199a增强AKT的表达

目的 探讨乙型肝炎病毒X蛋白(hepatitis B virus X protein,HBX)调节miR-199a的表达在HBV相关性肝癌发生中的分子机制.方法 重组HBX腺病毒(Ad-HBX)感染人肝癌HepG2细胞,HBX的siRNA转染稳定表达HBV基因的人肝癌HepG2.2.15细胞,荧光定量PCR方法检测HBV相关性肝癌组织标本和肝癌细胞中HBX、miR-199a、AKT的mRNA表达水平及运用Western blot检测肝癌组织标本和肝癌细胞中HBX、AKT的蛋白表达水平;分别用miR-199a mimics和miR-199a inhibitor的转染肝癌细胞后,荧光定量PCR方法检测肝癌细胞中AKT的mRNA表达水平及运用Westem blot检测肝癌细胞中AKT的蛋白表达水平.结果 miR-199a在HepG2.2.15细胞中表达水平显著低于HepG2细胞,并且证实其表达下调受到了HBX的调控(P＜0.05),HepG2.2.15细胞中AKT表达水平显著高于HepG2细胞(P＜0.05),在HepG2.2.15细胞中过表达miR-199a能明显下调AKT表达水平以及在HepG2细胞中抑制miR-199a能明显上调的AKT表达水平(P＜0.05),此外,miR-199a的表达量在HBV相关性肝癌组织比相应的癌旁组织表达降低.结论 HBX可以通过miR-199a调节AKT导致HBV相关性肝癌发生.     

CA724 is a novel factor for predicting the unresectability in pancreatic adenocarcinoma

This study aimed to assess the relationship between serum CA724 levels and the unresectability of pancreatic adenocarcinoma. A total of 302 patients with pancreatic adenocarcinoma were analyzed for the potential association between serum CA724 levels and the unresectability of pancreatic adenocarcinoma. Serum CA724 levels in patients with unresectable pancreatic adenocarcinoma were remarkably higher than those with resectable pancreatic adenocarcinoma (P < 0.001). Patients with elevated serum CA724 levels exhibited a 12.27-fold higher risk of unresectability than those with normal serum CA724 levels after adjusting for age, sex, and tumor location (95% CI = 5.28-28.51, P < 0.001). The analysis of receiver operating characteristics demonstrated that CA724 had superior predictive value to other tumor markers (AUC was 0.77 ± 0.03, 0.65 ± 0.04, and 0.62 ± 0.04 for CA724, CA125, and CA199, respectively). CA724 appeared to be a better predictor of unresectability than CA199 and CA125.