"增生平"治疗口腔扁平苔藓的临床疗效观察

目的:观察增生平对口腔扁平苔藓的治疗效果.方法:口腔黏膜扁平苔藓病例129例,其中糜烂型89例,萎缩型22例,斑块型18例,随机分为2组,增生平治疗组,口服增生平每日3次,每次4片,连续服用2～4个月;口炎清治疗组每日2次,每次10克冲服.结果:增生平治疗组总有效率为79.4%,口炎清治疗组62.1%,增生平治疗斑块型扁平苔藓有效率为55%,口炎清组22%.结论:增生平组治疗口腔扁平苔藓有效率高于口炎清治疗组,特别是对斑块型扁平苔藓有较好的疗效.     

Inhibitory effects of Zengshengping fractions on DMBA-induced buccal pouch carcinogenesis in hamsters

Background  Zengshengping (ZSP) tablets had inhibitory effects on oral precancerous lesions by reducing the incidence of oral cancer. However, the severe liver toxicity caused by systemic administration of ZSP limits the long-term use of this anti-cancer drug. The purpose of this study was to evaluate the tumor inhibitory effects due to the topical application of extracts from ZSP, a Chinese herbal drug, on 7, 12-dimethlbenz(a)anthracene (DMBA) induced oral tumors in hamsters. The study also investigated the anti-cancer mechanisms of the ZSP extracts on oral carcinogenesis.

Methods  DMBA (0.5%) was applied topically to the buccal pouches of Syrian golden hamsters (6–8 weeks old) three times per week for six weeks in order to induce the development of oral tumors. Different fractions of ZSP were either applied topically to the oral tumor lesions or fed orally at varying dosages to animals with oral tumors for 18 weeks. Tumor volume was measured by histopathological examination. Tumor cell proliferation was evaluated by counting BrdU labeled cells and by Western blotting for mitogen-activated protein kinase (MAPK) protein levels. The protein levels of apoptosis marker Caspase-3 and regulator Bcl-2 protein were also measured by Western blotting. 

Results  Our results showed that topical application of DMBA to the left pouch of hamsters induced oral tumor formation. Animals treated with DMBA showed a loss in body weight while animals treated with ZSP maintained normal body weights. Both the ZSP n-butanol fraction and water fraction significantly reduced tumor volume by 32.6% (P <0.01) and 22.9% (P <0.01) respectively. Topical application of ZSP also markedly decreased the BrdU-positive cell numbers in oral tumor lesions and reduced the expression level of mitogen-activated protein kinase (MAPK). In addition, ZSP promoted tumor cell apoptosis by increasing Caspase-3 expression but decreasing Bcl-2 protein production.

Conclusion  The n-butanol and water fractions of ZSP are effective at inhibiting tumor cell proliferation and stimulating apoptosis in oral cancer suggesting that these fractions have chemo preventive effects on DMBA induced oral carcinogenesis.

     

术中局灶化疗辅以增生平片治疗四肢骨巨细胞瘤21例临床观察

目的：手术中使用氨甲蝶呤、顺氯氨铂、阿霉素局灶化疗，手术后辅以增生平片口服以减少骨巨细胞瘤的复发，并保存患肢功能。方法：手术中彻底刮除病灶，瘤腔壁一次性用氨甲蝶呤灭活(氨甲蝶呤30mg NSl5ml-30ml浸湿明胶海绵均匀贴附于瘤壳内壁上)，植骨块用顺氯氨铂20-30mg NS50ml浸泡30min后充填瘤腔，植骨瘤腔置管隔日灌注氨甲蝶呤30mg，每2日一次共3次；充填骨水泥中一次性加入阿霉素80mg。术后口服增生平片，每日2次，每次8片，持续6个月。结果：治疗25例患者，2l例获随访，时间2-5年，2例复发。结论：术中使用氨甲蝶呤及顺铂或阿霉素局灶化疗，术后辅以增生平片口服对减少静止性及活动性骨巨细胞瘤的复发有一定作用。     

增生平对口腔癌预防作用的实验和临床研究

目的观察增生平对二甲基苯蒽(dimethyl-benzanthracene,DMBA)诱发的金黄地鼠口腔癌的阻断作用和对口腔癌前病变一口腔粘膜白斑的治疗效果.方法实验室研究50只雄性金黄地鼠,随机分为3组,阴性对照组10只,仅涂丙酮液.增生平治疗组和DMBA组各20只动物.DMBA组涂0.5%DMBA丙酮液,每周3次,共涂6周,治疗组DMBA同样处理后,每天给地鼠灌胃6.Og/kg剂量的增生平,治疗10周,进行肉眼和病理观察,同时进行微核、银染核仁组织区(Argentums Nucleolar Organizer Regions,AgNOR)和细胞增殖抗原(Proliferation Cell Nuclear Antigen,PCNA)检测.临床研究112名经临床和病理检查诊断为口腔粘膜白斑的患者随机分为治疗组59名和对照组53名.治疗组服用增生平,3次/日,每次4片,治疗8～12个月,对照组定期观察.治疗期间观察白斑病损变化.结果动物实验增生平组平均瘤数目和瘤负荷显著低于DMBA组,其抑制率分别为44.6%和69.6%,癌变率为55.0%,显著低于阳性对照组95.0%,实验组细胞微核发生率、AgNOR数目和PCNA标记指数均显著低于阳性对照组.临床治疗组有67.8%的患者白斑病损缩小,而对照组仅16.9%的患者白斑病损缩小.结论增生平可以使口腔癌前病变逆转,降低口腔癌的发生率.增生平对口腔粘膜白斑有一定的治疗作用.     

增生平片治疗食管上皮细胞增生108例

对食管癌癌前病变——食管上皮增生用增生平片进行临床治疗，治疗组在症状好转、导光镜检查及活检病理学检查好转率方面均优于对照组（Ｐ＜０．０１）；治疗组２个证候间比较，热瘀内结证疗效优于肝郁气滞证（Ｐ＜０．０５）。     

增生平片致肝损害

47岁女性患者,因患食管炎服用增生平3片,3次/d,半年后患者出现恶心,黄疸。其TBil122.2μmol/L,DBil108.0μmol/L,ALT581U/L,AST277U/L,GGT125U/L。停服增生平,患者经甘草酸二胺、茵栀黄、肌苷治疗8d后,症状明显好转,实验室检查:TBil94.7μmol/L,DBil81.2μmol/L,ALT162U/L,AST142U/L,GGT125U/L。患者症状明显好转。     

增生平片治疗肠上皮化生的临床疗效观察

目的：观察增生平片治疗肠上皮化生的临床疗效。方法：观察99例肠上皮化生患者服用增生平片12个月后肠化生逆转情况。结果：增生平片在肠上皮化生逆转的治疗中有效率59．6％，且不良反应低(1．98％)。结论：增生平片在胃粘膜肠化生的治疗中，有一定的疗效，且不良反应小、依从性好。     

Inhibitory effects of Zengshengping fractions on DMBA-induced buccal pouch carcinogenesis in hamsters

Background  Zengshengping (ZSP) tablets had inhibitory effects on oral precancerous lesions by reducing the incidence of oral cancer. However, the severe liver toxicity caused by systemic administration of ZSP limits the long-term use of this anti-cancer drug. The purpose of this study was to evaluate the tumor inhibitory effects due to the topical application of extracts from ZSP, a Chinese herbal drug, on 7, 12-dimethlbenz(a)anthracene (DMBA) induced oral tumors in hamsters. The study also investigated the anti-cancer mechanisms of the ZSP extracts on oral carcinogenesis.

Methods  DMBA (0.5%) was applied topically to the buccal pouches of Syrian golden hamsters (6–8 weeks old) three times per week for six weeks in order to induce the development of oral tumors. Different fractions of ZSP were either applied topically to the oral tumor lesions or fed orally at varying dosages to animals with oral tumors for 18 weeks. Tumor volume was measured by histopathological examination. Tumor cell proliferation was evaluated by counting BrdU labeled cells and by Western blotting for mitogen-activated protein kinase (MAPK) protein levels. The protein levels of apoptosis marker Caspase-3 and regulator Bcl-2 protein were also measured by Western blotting. 

Results  Topical application of DMBA to the left pouch of hamsters induced oral tumor formation. Animals treated with DMBA showed a loss in body weight while animals treated with ZSP maintained normal body weights. Both the ZSP n-butanol fraction and water fraction significantly reduced tumor volume by 32.6% (P <0.01) and 22.9% (P <0.01) respectively. Topical application of ZSP also markedly decreased the BrdU-positive cell numbers in oral tumor lesions and reduced the expression level of MAPK. In addition, ZSP promoted tumor cell apoptosis by increasing Caspase-3 expression but decreasing Bcl-2 protein production.

Conclusion  The n-butanol and water fractions of ZSP are effective at inhibiting tumor cell proliferation and stimulating apoptosis in oral cancer suggesting that these fractions have chemopreventive effects on DMBA induced oral carcinogenesis.