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1.
目的 观察微囊化牛嗜铬细胞(BCC)大鼠脑内移植的效果及微囊的作用.方法 将微囊化或非微囊化BCC和空微囊分别移植于帕金森病(PD)样大鼠脑纹状体内,观察术后阿朴吗啡诱发大鼠异常旋转行为的变化;用蔗糖一磷钾酸-乙醛酸(SPG)荧光染色和HE染色观察脑组织中植入的BCC及微囊的状态.结果 空囊组PD样大鼠异常旋转行为改变不明显(n=6,P>0.05);非微囊组16只PD样大鼠中9只大鼠移植后1周旋转圈数下降到移植前的44.0%~60.9%(P<0.01),移植6个月时仍有BCC在部分受体脑内存活,移植区有较明显的炎性反应,另有7只PD样大鼠移植后异常旋转行为无明显改善(P>0.05),其脑内未见存活的BCC,移植区有较明显的炎性反应;微囊组16只PD样大鼠移植后旋转圈数下降到移植前的17.6%~35.6%(P<0.01),移植后10个月时大鼠脑内仍存在大量存活的微囊化BCC,无明显的炎性反应.微囊化BCC移植改善PD样大鼠异常旋转行为的效果明显优于非微囊者(P<0.01).结论 BCC脑内移植可改善PD样大鼠的异常旋转行为;大鼠旋转行为的改善与BCC在脑内的存活状态有关;海藻酸钠及多聚赖氨酸制作的微囊可降低异种BCC移植的排斥反应发生率.  相似文献
2.
OBJECTIVE

Intraoperative analysis of microrecording data during pallidotomy often depends on subjective interpretation of the oscilloscope signal, especially during the analysis of phasic activity. The goals of this project were: 1) to develop an inexpensive system that allowed on-line, objective characterization of single-unit pallidal discharges, and 2) to have objective criteria to differentiate the internal part (GPi) from the external part (GPe) of the globus pallidus.

METHODS

A computer program was developed that allowed the analysis of firing rates (mean, median, and quartiles), spike count per unit time, and interspike interval (ISI) histograms with Chi-square statistical evaluation. Indices were developed that measured phasic activity, including burst index (BI) for the measurement of bursts, pause index (PI) for the measurement of pauses, and pause ratio (PR) for analysis of time spent in pauses. Single-unit activity of 152 GPe and 203 GPi cells in 47 Parkinson patients were digitized using the computer soundcard during pallidotomy and analyzed using this software.

RESULTS

GPe discharges had a mean firing rate = 42 Hz, BI = 0.81, PI = 0.21, and PR = 1.41. GPi had a mean firing rate = 81, BI = 1.61, PI = 0.04, and PR = 0.21. The PR was the best index that differentiated GPe from GPi, followed by PI, BI, and firing rates, in that order. Kinesthetic cells were recorded equally in GPe from GPi, and their responses to generalized movements were not significantly different.

CONCLUSION

(1) Signal analysis using the digitization process of a computer sound card and dedicated software is satisfactory for the objective “on-line” and “off-line” analysis of microrecordings (including phasic activity); (2) PI and PR are most helpful in differentiating neurons of GPi from those of GPe; (3) no single parameter can differentiate GPe from GPi activity in all cases; and (4) unlike the findings in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated monkeys, GPe and GPi of Parkinson patients have similar prevalence of kinesthetic cells and similar responses to generalized somatotopic effects.  相似文献

3.
Xue Y  Gao J  Xi Z  Wang Z  Li X  Cui X  Luo Y  Li C  Wang L  Zhou D  Sun R  Sun AM 《Artificial organs》2001,25(2):131-135
Bovine chromaffin cells were microencapsulated within alginate-polylysine-alginate (APA) membranes. Microencapsulated bovine chromaffin cells as well as unencapsulated cells and empty microcapsules were grafted into the brain of hemiparkinsonian rats with 6-hydroxydopamine (6-OHDA) lesions. Apomorphine-induced rotational behavior of the host animals and the survival of the grafted chromaffin cells were examined after transplantation. The animals receiving microencapsulated bovine chromaffin cells showed a significant decrease (17.6--35.6%) in apomorphine-induced rotation 1 week postimplantation that remained stable for the 10 month test period. Fluorescent histochemistry further revealed that microencapsulation increased the chromaffin cell survival with only a minimum host reaction for up to 10 months posttransplantation while the survival of free, unencapsulated chromaffin cells was only modest and was accompanied by a large inflammatory response. The reduction of apomorphine-induced rotations was correlated with the survival of bovine chromaffin cells in the host brain. The data indicate that encapsulation of bovine chromaffin cells in APA membranes reduces the host immune response to the xenograft and prolongs the viability of the grafted cells.  相似文献
4.
苍白球腹后部切开术治疗帕金森病异动症的疗效分析   总被引:5,自引:0,他引:5  
目的 分析苍白球腹后部切开术(PVP)对帕金森病(PD)患者中左旋多巴所诱导的异动症的治疗效果,评估手术的临床治疗意义。方法 采用国际上公认的PD综合评分法9UPDRS)对36例异动症患者的表现进行定量分,就异动症的持续时间、严重程度(致残度)以及整体影响做PVP术前术后的统计比较。结果 术后1周内症状的整体改善率为76.2%。其中手术对侧异动症持续时间减少88.8%;致残度改善79.7%。经统计  相似文献
5.
Summary. Background: The long-term neuropsychological and quality of life (QOL) outcomes of unilateral thalamic deep brain stimulation (DBS) in patients with intractable Parkinson's disease (PD) have not heretofore been described. Method: Six patients diagnosed with PD underwent unilateral DBS implantation into a verified thalamic VIM nucleus target. Participants completed presurgical neuropsychological evaluation and follow-up assessment at approximately one year postsurgery. Findings: Compared to their presurgical scores, PD patients exhibited significant improvement on measures of conceptualization, verbal memory, emotional adjustment, and QOL at one-year follow-up. A few nominal declines were observed across the battery of tests. Interpretation: These data provide preliminary support for the long-term neurocognitive safety and QOL improvements following thalamic stimulation in patients with PD.  相似文献
6.
微电极导向腹后内侧苍白球毁损治疗帕金森病   总被引:4,自引:0,他引:4  
目的 探讨微电极导向腹后内侧苍白球毁损手术治疗帕金森病的方法、效果与评价。方法 通过微电极细胞电生理启示技术对CT定位靶点进行分析、确认和调整,对109例帕金森病患者分别行单侧一内侧苍白球毁损术(45例)、同期双侧腹后内侧苍白球毁损术(21例)、同期腹后内侧苍白和丘脑Vim核联合损术(43例)。患者术前及术后不同状态改良Webster法记分,分别计算改善率,并进行疗效评估。结果 经微电极确认后的电  相似文献
7.
Chronic anterior pallidal stimulation for parkinson's disease   总被引:4,自引:0,他引:4  
Summary We present the case of a 51-year-old female who had a four-year history of Parkinson's disease with severe on-off and disabling progression of symptoms on chronic levodopa therapy.After obtaining FDA approval, we implanted a Medtronic deep brain stimulation lead stereotactically into the right anterior pallidum contralateral to her most Symptomatologic side. Intra-operative stimulation trials at 100 Hz caused reproducible reversal of akinetic symptoms and simultaneous microelectrode recording of the posteroventral pallidum revealed decreased neural activity during anterior pallidal stimulation.The patient was evaluated pre-operatively and postoperatively using the Hoehn and Yahr Staging Scale, the Unified Parkinson's Disease Rating Scale (UPDRS), videotape, and a computerized data glove. Six months after implantation, the total UPDRS score was decreased from 68 to 8 and Hoehn and Yahr Staging improved from 3.0 to 1.5 during periods of chronic high frequency stimulation. Dramatic improvements in tremor, dystonia, bradykinesia, and akinesia were noted within seconds of stimulator activation and were also objectively measured using a computerized data glove. This case reveals the potential for therapeutic pallidal stimulation for Parkinson's akinetic symptomatology.  相似文献
8.
目的建立立体定向手术下6-OHDA黑质毁损的帕金森病恒河猴模型并建立客观评估体系。方法实验用恒河猴5只,在立体定向技术下以6-OHDA对恒河猴右侧黑质进行7个靶点的毁损,术后2周、2、4、6个月进行行为学评分量化,于术后7个月以SPECT检测恒河猴双侧纹状体DAT的代谢来判断毁损侧DA代谢减少程度,术后8个月以TH免疫荧光法检测恒河猴双侧黑质的DA能神经元改变,以此来证实黑质的毁损程度。结果术后7个月SPECT检测显示毁损侧纹状体区DA代谢仅为对侧的10%~25%左右,术后8个月双侧黑质的TH免疫荧光检测显示毁损侧黑质DA能神经元减少70%以上。结论立体定向技术下6-OHDA单侧恒河猴黑质毁损方法能够成功的制作符合人类病理特征的帕金森病模型。SPECT是评价模型制作成功与否的客观手段。  相似文献
9.
体外培养的多巴胺能神经细胞最佳移植时机初探   总被引:3,自引:0,他引:3  
目的 探索体外培养的多巴胺能神经细胞的最佳移植时机,为细胞移植治疗帕金森病奠定基础。方法 体外扩增E11胎鼠腹侧中脑神经前体细胞7 d后,分别在诱导分化期开始0、1、2、3、4、5、6、7 d时收获细胞,重新接种后再培养7 d。根据多巴胺能神经元生存率高低,初步决定出最佳移植时机。结果 在诱导分化期开始0 d和1 d传代细胞后,多巴胺能神经元的生存率显著高于其他时间点。结论 E11胎鼠腹侧中脑神经前体细胞体外扩增后诱导分化0 d和1 d时,可能是最佳移植时机。  相似文献
10.
男性性功能障碍与脊髓损伤及其他神经系统疾病   总被引:3,自引:0,他引:3  
男性性功能是人与环境之间错综复杂的相互作用过程。性刺激的认识整合和生理反应必须依赖于神经系统的功能。因此 ,中枢或外周神经功能紊乱也许会损伤男性性功能。本文对男性性活动的正常的神经解剖学、流行病学、病理生理学 ,及由于脊髓损伤、脑血管疾病、多发性硬化和帕金森病等引起的性功能障碍进行了综述  相似文献
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