Interplay of oxidative stress and antioxidant bio markers in oil adjuvant Brucella melitensis vaccinated and challenged mice

The intracellular nature of Brucella leads to rise in oxidative stress due to bacterial invasion, particularly at the site of predilection spleen and lymph nodes. The present study aimed to evaluate the erythrocytic and tissue specific oxidative stress responses induced during oil adjuvant killed Brucella melitensis vaccination. The results of the study clearly implicated a significant increase in level of catalase, and superoxide dismutase (SOD) activity and lipid peroxidation (LPO), and total protein content in erythrocytes after vaccination. The activity of glutathione-S-transferase (GST) was unaltered during the period of experiment. The catalase activity and GSH content was significantly increased in lung and spleen tissues. The tissues GST levels increased significantly in all tissues, while tissue SOD level increased significantly only in lung tissues. Thus, it can be inferred that oil adjuvant based Brucella vaccine induces negligible signs of inflammatory pathophysiology and supports the development of significant level of protection against virulent Brucella challenge.     

复方丁香开胃帖减轻胃癌术后腹胀的临床观察与护理

目的观察使用复方丁香开胃贴对胃癌术后患者减轻腹胀的临床效果及护理。方法将101例胃癌术后且均为胃大部分切除术的患者,将其随机分为对照组50例,治疗组51例,2组患者均被告知和协助术后第一天离床活动,治疗组的患者使用复方丁香开胃贴外贴于患者的脐部即神阙穴,密切观察患者的腹胀情况,比较2组患者术后腹胀的程度及肛门排气情况和住院时间。结果治疗组病例术后腹胀程度、肛门排气情况与对照组相比,差异均有统计学意义(P<0.05)。结论胃癌术后的患者使用复方丁香开胃贴可以有效的减轻腹胀,缩短肛门排气时间,减少住院时间,使患者早日康复出院。     

艾灸合暖宫贴干预寒湿凝滞型痛经的临床观察

目的探讨艾灸合暖宫贴对寒湿凝滞型痛经女大学生痛经症状、疼痛程度和睡眠质量的影响。方法选择寒湿凝滞型痛经女大学生80例,随机分为西药组和艾灸合暖宫贴组,每组40例,共观察4个月经周期。采用中医痛经症状评分、疼痛视觉模拟评分(visual analogue scale,VAS)和匹兹堡睡眠质量指数评分(Pittsburgh sleep quality index,PSQI),评定患者治疗前、第3个月经周期(治疗期间)、第4个月经周期(停止治疗)的痛经症状、疼痛程度和睡眠质量的变化情况。结果治疗后的第3个月经周期,两组的中医痛经症状评分、VAS评分和PSQI评分均较前明显下降(P<0.05)。第4个月经周期,停止治疗后,西药组的中医痛经症状评分、VAS评分较第3个月经周期上升(P<0.05),但较治疗前评分降低(P<0.05);艾灸合暖宫贴组中医痛经症状评分、VAS评分和PSQI评分与西药组比较,差异有统计学意义(P<0.01)。结论艾灸合暖宫贴可以改善寒湿凝滞型痛经女大学生的痛经症状、疼痛程度和睡眠质量,疗效具有持久性,值得推广应用。     

车前子酒炙工艺优选及其不同炮制品对比研究

[目的] 优选酒炙车前子的最佳炮制工艺，并对车前子生品、酒炙品、盐炙品进行对比研究。[方法] 以车前子中京尼平苷酸和毛蕊花糖苷的含量为考察指标，选择加酒量、闷润时间、炒制温度、炒制时间为考察因素，通过单因素实验优选车前子的最佳酒炙工艺。采用高效液相色谱-二极管阵列检测器（HPLC-DAD）建立车前子水提液指纹图谱测定方法，通过指标成分含量、不同炮制品水提液的出膏率及指纹图谱相似度分析不同炮制品间的差异。[结果] 酒炙车前子的最佳炮制工艺为每100 g车前子加黄酒10 g，闷润60 min，150℃下炒制9 min。经盐炙和酒炙后，车前子中的京尼平苷酸和毛蕊花糖苷的含量均较生品显著增加，而两种炮制品并无统计学差异；在出膏率方面，盐炙和酒炙车前子的出膏率无明显差异，但均高于生车前子。高效液相色谱（HPLC）指纹图谱显示车前子生品、盐炙品和酒炙品具有很好的相似度。[结论] 优选出的车前子酒炙工艺稳定可行，并与盐车前子在成分上具有一致性，鉴于酒车前子已不再流通，在经典名方的开发研究中，可考虑用现行通用的盐车前子取代。     

Alleviating effect of 24- epibrassinolide on seed oil content and fatty acid composition under drought stress in safflower

Brassinosteroids may importantly affect safflower (Carthamus tinctorius L.) growthand quality under stress. The oil responses of three spring safflower genotypes (Goldasht, Faraman and Sina) affected by the exogenous application of 24- epibrassinolide (EBL, 0.1 μM) under drought stress (85 (I1), 65 (I2) and 45 % (I3) of the field capacity) were investigated in 2016. The stress decreased oil percentage and yield, fatty acid yield and stomatal conductance, whereas supplying sufficient water did not alter fatty acid content. EBL increased oil and fatty acid percentage (except palmitic acid), and stomatal conductance. Foliar EBL application led to enhancement of oleic (15.95 %), linolenic (22.50 %), linoleic (4.26 %) and stearic acid (29.6 %) compared with control. The hormonal treatment also enhanced oil and fatty acid yield in I1 and I2. The desaturation efficiency and unsaturation indexes indicated that EBL can affect safflower response under the stress by desaturating 18:2 fatty acids to 18:3 fatty acids. Safflower oil content and fatty acids, as a function of plant genotype, can be enhanced under drought stress using EBL. The presented research can also further enhance our understanding related to lipid metabolism in safflower affected by EBL under drought stress. Such results are of nutritional, economic, industrial and environmental significance.     

巧用3L 透明输液贴改良腔镜器械

近年来，腹腔镜下脾切除加贲门周围血管离断术逐步成熟，越来越多的临床医师开始应用这项技术［1］。我院对腹腔镜脾切除技术进行了改良，使腹腔镜手术的创伤更小，更能显示微创优势，并进行了相关报道［2］。我院手术室将3 L透明输液贴应用于该类手术，取得了良好效果，现报告如下。     

Electrochemical properties of a tetrabromo-p-benzoquinone modified carbon paste electrode. Application to the simultaneous determination of ascorbic acid,dopamine and uric acid

The electrochemical study of a tetrabromo-p-benzoquinone modified carbon paste electrode (TBQ-MCPE), as well as its efficiency for electrocatalytic oxidation of ascorbic acid, dopamine and uric acid, is described. Cyclic voltammetry was used to investigate the redox properties of this modified electrode at various solution pH values and at various scan rates. Three linear segments were found with slope values of ?58.4 mV/pH, ?28.1 mV/pH and 0.0 mV/pH in the pH range 2.0–7.1, pH 7.1–9.0 and pH 9.0–11.0, respectively. The apparent charge transfer rate constant, k_s, and transfer coefficient, α, for electron transfer between TBQ and CPE were calculated as 3.79 ± 0.10 s^?1 and 0.55, respectively. The electrode was also employed to study the electrocatalytic oxidation of AA, using cyclic voltammetry, chronoamperometry and differential pulse voltammetry as diagnostic techniques. It has been found that the oxidation of AA at the surface of TBQ-MCPE occurs at a potential of about 430 mV less positive than that of an unmodified CPE. The diffusion coefficient of AA was also estimated using chronoamperometry. The kinetic parameters such as the electron transfer coefficient, α, and heterogeneous rate constant, $k_{\mathrm{h}}^{\prime }$, for oxidation of AA at the TBQ-MCPE surface was determined using cyclic voltammetry. Differential pulse voltammetry (DPV) exhibits two linear dynamic ranges and a detection limit of 0.62 μM for AA. In DPV, the TBQ-MCPE could separate the oxidation peak potentials of AA, DA and UA present in the same solution, though at the unmodified CPE the peak potentials were indistinguishable. This modified electrode was quite effective not only to detect AA, DA and UA, but also in simultaneous determination of each component concentration in the mixture.     

氢氧化钙甘油糊剂对根管壁玷污层的溶解作用研究

目的观察氢氧化钙甘油糊剂对根管壁玷污层的溶解作用。方法30颗离体上前牙随机分为3组。常规预备根管后,A组封入氢氧化钙甘油糊剂,B组封入碘仿糊剂,C组封入樟脑酚。7d后清洗根管壁,并在扫描电镜下观察。结果A组根管壁玷污层明显较少,与B组和C组差异有统计学意义(P<0.01);B组与C组玷污层差异无统计学意义(P>0.05)。结论氢氧化钙甘油糊剂对根管壁玷污层有溶解作用。     

Do buffered local anesthetics provide more successful anesthesia than nonbuffered solutions in patients with pulpally involved teeth requiring dental therapy?: A systematic review

Background

The authors conducted a systematic review that addresses the following population, intervention, comparison, outcome question: “In adults requiring dental therapy with pulpally involved teeth, what is the comparative efficacy of buffered local anesthetics (LAs) compared with that of nonbuffered LAs in achieving anesthetic success?”

Vitapex药物糊剂充填乳牙根管的疗效观察

目的观察Vitapex糊剂充填乳牙根管的疗效.方法对120名儿童140个患牙进行根管治疗术,分为两组.实验组用Vitapex糊剂充填根管,对照组用氧化锌丁香油糊剂充填根管.结果术后追踪观察1～2年,实验组观察68个乳牙,成功65个(95.59%);对照组观察64个乳牙,成功52个(81.25%).结论用Vitapex糊剂充填乳牙感染根管疗效较好.