Silencing KLF16 inhibits oral squamous cell carcinoma cell proliferation by arresting the cell cycle and inducing apoptosis

Krüppel-like factor 16 (KLF16), a member of the Krüppel-like factor (KLF) family, has been extensively investigated in multiple cancer types. However, the role of KLF16 in oral squamous cell carcinoma (OSCC) remains unknown. Thus, we conducted this study to investigate its related mechanism. KLF16 expression in OSCC cell lines was quantified by western blotting. Then, OECM1 and OC3 cells were divided into Blank, siCtrl, siKLF16#1 and siKLF16#2 groups. Subsequently, cell proliferation was detected using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assays, cell migration and invasion were detected with wound healing and Transwell assays, and cell cycle distribution and cell apoptosis were detected via flow cytometry. KLF16, p21, CDK4, Cyclin D1 and p-Rb expression was detected by western blotting. Finally, xenograft models were established in nude mice to observe the in vivo effects of KLF16 on OSCC. KLF16 protein expression was upregulated in OSCC cells. Compared to the cells in the Blank group, the OECM1 and OC3 cells in the siKLF16#1 group and siKLF16#2 group exhibited a sharp decrease in proliferation but a remarkable increase in apoptosis. Moreover, the proportion of cells in the G0/G1 phase notably increased and that in the S phase decreased, with evident decreases in cell invasion and migration. Moreover, KLF16, cyclin-dependent kinase 4 (CDK4), Cyclin D1 and p-Rb protein expression was upregulated, but p21 expression was downregulated. The mice in the siKLF16#1 and siKLF16#2 xenograft model groups exhibited slower tumour growth and smaller tumours with evident downregulation of Ki67 expression compared to the mice in the Blank group. KLF16 expression was upregulated in OSCC cells, and interfering with KLF16 led to cell cycle arrest, inhibited OSCC cell growth and promoted cell apoptosis.     

刍议清络法论治特发性肺纤维化急性发作＊

本文通过对特发性肺纤维化急性发作病因病机的分析，认为其病因为外邪或内伤引动肺络虚火，从阳化热，病机为“肺热”、“络瘀”；并结合其病因病机，探究归纳《温病条辨》中清络法和中药复方清络饮在论治特发性肺纤维化急性发作时的理论基础，为临床中论治特发性肺纤维化急性发作，改善患者症状提供新方法，为肺络病证治体系构建及应用提供新思路。     

新型胸骨环抱器结合钢丝固定胸骨的临床效果

目的探讨新型胸骨环抱器结合钢丝固定胸骨在成人心脏正中切口直视手术中的应用效果。 方法回顾性分析2016年1月至2017年12月期间在临沂市人民医院心脏血管外科接受手术治疗的328例心脏大血管疾病患者临床资料，按胸骨固定方法不同分为对照组159例和研究组169例。其中对照组采用传统的钢丝将锯开的胸骨左右两侧缝合，后收紧钢丝、合拢胸骨并拧紧钢丝，予以固定胸骨；研究组采用钢丝对合锯开胸骨的基础上放置新型环抱器固定胸骨。分析2组患者关胸时间(关胸开始到皮肤切口缝合结束的时间)、二次开胸止血(胸骨相关)率、术后24 h引流量、术后切口、胸骨感染或胸骨裂开率、术后胸痛不适发生率以及术后住院时间。患者出院后通过门诊、电话询问进行随访。数据比较采用t检验和χ²检验。 结果关胸术后，对照组1例患者因急性A型主动脉夹层术后急性肾功能衰竭死亡，其余所有患者均痊愈出院。研究组患者关胸时间(32.3±7.3) min，明显少于对照组(51.5±8.4) min，差异有统计学意义(t＝－22.113，P<0.05)。胸骨相关的二次开胸止血，研究组发生1例，而对照组发生6例，2组比较差异有统计学意义(χ²＝3.969，P＝0.046)。研究组术后24 h引流量(372.8±213.1) mL，少于对照组(538.9±202.6) mL，2组比较差异有统计学意义(t＝－7.224，P<0.05)。研究组术后未见切口和胸骨感染和(或)裂开，而对照组感染和(或)裂开共7例，其中胸骨裂开4例，早期予以新型环抱器重新固定胸骨，治愈出院；切口感染2例，予以换药后重新缝合切口后治愈；胸骨感染1例，予以胸骨清创加胸大肌肌瓣转移手术后康复出院，2组比较差异有统计学意义(χ²＝7.599，P<0.05)。研究组术后出现胸痛不适2例，而对照组7例，2组比较差异有统计学意义(χ²＝4.101，P＝0.043)。研究组患者术后住院时间(8.6±4.1) d，少于对照组(10.5±3.4) d，2组比较差异有统计学意义(t＝－4.467，P<0.05)。患者出院后随访(11.3±4.9)个月，所有患者均未见胸骨感染及裂开。 结论新型胸骨环抱器结合钢丝固定胸骨手术切口，与传统手术相比操作简单、固定牢固，在缩短关胸手术时间、减少术后切口并发症、缩短住院时间等方面优势明显，可以广泛使用于成人心脏外科正中手术切口。     

The Chinese National Twin Registry: a ‘gold mine’ for scientific research

The Chinese National Twin Registry (CNTR) currently includes data from 61 566 twin pair from 11 provinces or cities in China. Of these, 31 705, 15 060 and 13 531 pairs are monozygotic, same‐sex dizygotic and opposite‐sex dizygotic pairs, respectively, determined by opposite sex or intrapair similarity. Since its establishment in 2001, the CNTR has provided an important resource for analysing genetic and environmental influences on chronic diseases especially cardiovascular diseases. Recently, the CNTR has focused on collecting biologic specimens from disease‐concordant or disease‐discordant twin pairs or from twin pairs reared apart. More than 8000 pairs of these twins have been registered, and blood samples have been collected from more than 1500 pairs. In this review, we summarize the main findings from univariate and multivariate genetic effects analyses, gene–environment interaction studies, omics studies exploring DNA methylation and metabolomic markers associated with phenotypes. There remains further scope for CNTR research and data mining. The plan for future development of the CNTR is described. The CNTR welcomes worldwide collaboration.     

同型半胱氨酸对高血压患者脑卒中复发风险的影响

目的探讨高血压合并脑卒中患者的血浆同型半胱氨酸（Hcy）水平与其他危险因素对于脑卒中复发的影响。 方法分析徐州市中心医院心内科和徐州医科大学附属医院神经外科自2012年5月至2013年12月收治的1623例高血压脑卒中患者的基线资料，中位随访4.9年，根据随访事件中是否发生脑卒中分为复发组（312例）与未复发组（1311例）。Kaplan-Meier生存分析比较不同危险因素脑卒中复发率的差异，单因素与多因素Cox回归模型分析影响脑卒中复发的独立危险因素，以及危险因素之间的交互作用。 结果复发组年龄、空腹血糖、Lg Hcy的水平，以及糖尿病、房颤的患病率均高于未复发组（P<0.05）。Kaplan-Meier生存分析显示，糖尿病、房颤、年龄≥60岁、空腹血糖≥7.0 mmol/L、Hcy≥15 μmol/L的脑卒中复发率明显升高（Log-rank检验，P<0.05）。多因素Cox回归模型分析显示，高龄、Lg Hcy水平升高，以及房颤、糖尿病是脑卒中复发的独立危险因素。Lg Hcy分别与糖尿病、空腹血糖、年龄存在交互作用。 结论血浆Hcy水平升高既是高血压合并脑卒中患者卒中复发的独立危险因素，又通过与糖尿病、高龄、空腹血糖水平升高的交互作用显著增加脑卒中复发风险。     

浅低温不停循环优先重建弓部血管结合术中支架阻断技术与传统孙氏手术的对比研究

目的:采用浅低温不停循环优先重建弓部血管结合术中支架阻断技术治疗急性Stanford A型主动脉夹层,并与传统孙氏手术进行比较,评估其效果和安全性。方法:回顾性分析2017年1月至2018年12月山东省临沂市人民医院心脏血管外科手术治疗的40例急性A型主动脉夹层患者的资料,其中21例在浅低温不停循环下,采用优先重建弓部血管结合术中支架阻断技术进行改良的孙氏手术治疗,19例采用传统的孙氏手术治疗。统计分析患者术中、术后的相关指标及并发症情况。结果:与传统的孙氏手术相比,改良组在术中阻断时间[(218.63±27.86)min比(101.52±17.23)min]、停循环时间[(1174.74±183.65)s比(43.19±8.32)s]、体外循环时间[(218.63±27.86)min比(194.19±24.25)min]、术后并发症(14次比6次)、术后呼吸机辅助时间[(72.89±45.57)h比(29.86±32.3)h]及围术期输血量[(1332.63±301.55)ml比(991.90±323.23)ml]等方面存在明显优势,差异有统计学意义(P均<0.05)。手术后清醒时间方面,改良组优于传统组,但差异无统计学意义。结论:采用浅低温不停循环优先重建弓部血管结合术中支架阻断技术治疗急性A型主动脉夹层是安全、有效的,可以明显改善患者预后,减少并发症的发生。