2001～2005年《中国妇幼健康研究》载文被引分析

目的 从文献引证角度透视《中国妇幼健康研究》的论文学术水平、期刊质量和影响力.方法 依据《中国生物医学期刊引文数据库》,利用科学文献计量学方法对《中国妇幼健康研究》载文被《中国生物医学期刊引文数据库》来源期刊引用情况进行统计分析及评价.结果 2001～2005年平均发文被引比例为34.43%,单篇论文平均被引2.11次,单篇论文最高被引为18次.单篇被引4次以上论文42篇,共被引266次,占总被引篇数的14.89%,占被引总频次的41.24%.645篇被引论文中,共有作者274人,作者最高被引篇数为5篇.被引1篇的作者238人,占作者总数的86.86%.有278种期刊引用《中国妇幼健康研究》,自引率为0.028.结论 《中国妇幼健康研究》所载文献质量较高,形成具有独立风格和特色且相对稳定的研究体系.该刊不仅是我国妇幼卫生健康研究领域重要的信息源之一,受到同行较高程度的关注,也是我国医学领域的主要核心期刊.     

高温热塑充填技术对后牙弯曲根管根尖封闭能力的实验研究

目的评价和比较冷牙胶侧方加压充填技术和高温热塑充填技术对后牙弯曲细小根管根尖孔的封闭能力。方法选取32个工作长度为8~10 mm的新鲜离体后牙弯曲根管作为研究对象,16个采用冷牙胶侧方加压技术进行充填,16个采用高温热塑充填技术(ObturaⅡ)。设阳性与阴性对照,采用染色法比较根尖封闭能力。结果两种充填技术根尖微渗漏差异有统计学意义,ObturaⅡ组明显低于冷侧压组(P<0.05)。结论高温热塑充填技术对后牙弯曲根管具有良好的根尖封闭效果。     

Discordant time trends in maternal body size and offspring birthweight of term deliveries in France between 1972 and 2003: data from the French National Perinatal Surveys

We investigated time trends in maternal weight before and during pregnancy and in infant birthweight in France, from 1972 to 2003, using data on singleton live term births from the representative National Perinatal Surveys of 1972, 1981, 1995, 1998 and 2003 (n=8,664, 4,494, 11,445, 12,006, 12,692, respectively). Mothers were interviewed a few days after delivery and data on delivery and the newborn were extracted from hospital records. Maternal prepregnancy weight, height, body mass index and pregnancy weight gain all increased from 1972 to 2003; however, birthweight did not show a parallel trend. After taking gestational age, maternal age, parity, country of origin, newborn gender and maternal smoking during pregnancy into account, mean birthweight increased between 1972 and 1995 but decreased thereafter and, consistently, there was an increase in small-for-gestational age (SGA) and a decrease in large-for-gestational age newborns. Further adjustment for induced delivery, an indicator of obstetric practice, did not change the results. A similar variation has been observed very recently in the US and in Germany. Further research is needed to identify the factors responsible for these discordant changes and especially the factors responsible for the recent increase in SGA since this has been shown to be associated with poorer health in later life.     

子宫内铅暴露对仔鼠牙齿萌出和釉质发育的影响

目的观察母鼠妊娠期间不同剂量铅暴露对仔鼠牙齿萌出情况和釉质发育的影响。方法27只怀孕SD大鼠随机分为铅暴露高剂量组、低剂量组和对照组,每组9只。铅暴露组饮用去离子水中加入醋酸铅进行染毒[以铅(Pb2+)含量计算高剂量组200mg/L、低剂量组50mg/L],对照组饮用去离子水。染毒自大鼠孕第1天持续至自然分娩。仔鼠出生后第26天在下切牙龈乳头水平进行第1次标记,并于出生后第36天在同一牙龈乳头水平行二次标记。第2次标记当日取全血测定血铅并处死仔鼠。测定切牙铅含量,应用立体显微镜观察牙齿形态并测量切牙两次标记间距离,应用电子探针测定切牙釉质钙、磷含量并计算比值。结果高、低剂量铅暴露仔鼠组血铅较对照仔鼠组增高,差异有统计学意义(P<0.01);高、低剂量铅暴露仔鼠组齿铅[(77.3±6.3)、(27.8±4.5)μg/g]与对照仔鼠组[(6.6±0.8)μg/g]相比,差异有统计学意义(P<0.01)。铅暴露仔鼠组切牙较小,牙尖磨耗明显并多见舌侧髓腔暴露,高铅剂量组更为明显。高、低剂量铅暴露仔鼠组切牙萌出速率[(0.25±0.08)、(0.30±0.09)mm/d]与对照仔鼠组[(0.39±0.09)mm/d]比较,铅暴露组萌出较为缓慢,三组间差异有统计学意义(P<0.01)。仔鼠釉质钙/磷比分析显示,随铅染毒剂量的增加,钙/磷比(1.68±0.54、1.37±0.47)降低,     

Acute myeloblastic leukemia with active tyrosine protein kinase

High level of tyrosine protein kinase activity was found in a membrane fraction isolated from an acute myeloblastic leukemia, out of 24 leukemias of different origin investigated. The major substrate for tyrosine phosphorylation in vitro was a 58 kDA protein (p58). The phosphorylation proceeded actively at 0 degrees C and was strongly stimulated by Mn2+ ions. Comparison by partial proteolysis of the p58 with similar phosphoproteins from a T-lymphoma line (KE37) and from lectin stimulated lymphocytes showed high similarity. The possible role of the tyrosine kinase activity in this leukemia is discussed.     

N263萃取-偶氮肿Ⅲ分光光度法测定建材样品中微量钍

本文介绍了碱熔样品,RGTA-TEA掩蔽分离大量基体元素,用N₂₆₃萃取-分光光度法测定建材样品中微量钍的方法。对萃取酸度,干扰离子,回收率等影响准确度的因素进行了研究。     

草苔虫内酯对SD大鼠致畸作用的研究

目的：探讨草苔虫内酯对SD大鼠的致畸作用。方法：采用大鼠标准致畸试验,孕SD大鼠随机分为4组,每组16～17只。草苔虫内脂高、中、低剂量实验组分别按0.016、0.008、0.004mg/kg给药,对照组给予0.9%生理盐水。大鼠于孕期第6～15天按10ml/kg体重连续尾静脉注射给药。孕20天处死孕鼠,检查母体妊娠与胎鼠畸形情况。结果：中、高剂量组的孕鼠增重分别为67.72、11.98g,与对照组比较明显减少（P〈0.05）,并具有一定的剂量_反应趋势。在高剂量下,活胎率为61.42%,与对照组比较明显降低（P〈0.05）;吸收胎率和死胎率分别为20.81%、17.77%,与对照组比较明显增高（P〈0.05）。各受试剂量下,活胎体质量、活胎身长较对照组明显减少和降低（P均〈0.05）。各实验组均未观察到胎鼠明显的外观、脏器以及骨骼的畸形。结论：草苔虫内酯在中、高剂量下具有母体毒性,在高剂量下有胚胎毒性。在受试剂量下有一定的胎儿毒性,但无明显的致畸作用。     

牛血清游离氟成分分析标准物质研制

根据地方性氟中毒防治、科研和监测工作中对氟测定质量控制的要求,研制了牛血清游离氟成分分析标准物质。进行标样制备及均匀性和稳定性检验;国内5家实验室采用3种不同原理分析方法对标样定值;定值数据统计处理,确定游离氟的保证值和不确定度。同时给出标准物质总氟参考值。研制结果表明标准物质均匀性符合要求,稳定性超过一年,量值结果准确可靠。     

Fetal and Maternal Velocimetry in High Risk Pregnancies for the Assessment of Adverse Perinatal Outcome

Pulsed-Doppler ultrasound assessment of blood flow was performed simultaneously in the fetal middle cerebral artery, umbilical artery, and uteroplacental vessels to verify the incidence of velocimetric abnormalities, as well as their predictive value towards adverse perinatal outcome. Eighteen normal pregnancies and 32 complicated pregnancies (intrauterine growth retardation, pregnancy-induced hypertension, chronic hypertension and pre-eclampsia) have been examined between 30th and 40th week of gestation. Waveform abnormalities were detected even in the control group, more frequently in the cerebral artery (11.1%) and umbilical artery (5.5%) than in uteroplacental arteries. Umbilical artery abnormalities were found in intrauterine growth retardation, pregnancy-induced hypertension, and preeclampsia; while the highest number of anomalous Doppler waveforms in the middle cerebral artery was seen in 71.4% of patients with pregnancy-induced hypertension. Pathological umbilical artery patterns have shown a diagnostic accuracy of 62% in identifying adverse perinatal outcome. Both middle cerebral artery and uteroplacental vessels demonstrated a diagnostic accuracy of 58%. The false positive rate is 6% for the umbilical artery, 8% for the middle cerebral artery, and 0% for uteroplacental vessels, contrary to current methods of fetal surveillance (such as nonstress test, contraction stress test and biophysical profile), which have a false-positive rate ranging from 30% to 70%.