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排序方式: 共有81条查询结果,搜索用时 31 毫秒
1.
目的 探讨脑脊液中肿瘤坏死因子α(TNF-α)、β2微球蛋白(β2M)和铁蛋白(F)对于中枢神经系统白血病(CNSL)诊断的意义.方法 采用酶联免疫吸附和放射免疫方法检测CNSL组,无CNSL组和对照组脑脊液中TNF-α、β2M和F的变化.结果 CNSL组脑脊液中TNF-α、β2M和F均高于无CNSL组和对照组,有显著性差异(P<0.05)).TNF-α、β2M和F联合检测CNSL的约登指数均高于单一指标检测CNSL.结论 脑脊液中TNF-α、β2M和F在CNSL的诊断中有重要意义,提示其联合检测可以早期诊断CNSL.  相似文献
2.
Ferritin immunohistochemistry as a marker for microglia   总被引:1,自引:1,他引:0  
Summary An immunohistochemical analysis of formalin-fixed, paraffin-embedded brain sections was performed with antisera against holoferritin and the light(L)-subunit of ferritin. Sections immunostained using anti-glial fibrillary acidic protein (GFAP), Ricinus communis agglutinin-1 (RCA-1) stain for microglia and iron stain (Berlin blue stain) were compared. The L-subunit of ferritin was purified from normal human spleen according to the modified scrapie-associated fibrils purification, and the antiserum was raised in a rabbit. Both ferritin antisera positively stained resting and, more markedly, reactive microglia, both of which were also stained with RCA-1 but not with GFAP. Ferritin-positive resting microglia were seen more abundantly in cerebral and cerebellar cortices than in white matter. The advantages of ferritin antisera over RCA-1 are as follows. (1) RCA-1 heavily stains blood vessels, while anti-ferritin does not, hence the microglial cells are more readily visualized with ferritin immunohistochemistry. (2) Reactive microglia and macrophages are more strongly stained with anti-ferritin. (3) The staining intensity of ferritin is independent of the length of tissue fixation in formalin. However, anti-ferritin is inferior to RCA-1 in staining resting microglia with a scanty cytoplasm, especially in the white matter, probably because the former recognizes cytoplasmic components, while the latter recognizes cell membrane. Iron stain only gave a reaction to microglial cells in brains with neurosyphilis and to hemosiderin-laden macrophages. Thus, in addition to RCA-1, ferritin antisera are useful as a microglia marker in formalin-fixed, paraffin-embedded sections.Supported in part by Dr. A. Kondo, Department of Neuropathology, Neurological Institute, Kyushu University  相似文献
3.
H-ferritin is the major source of iron for oligodendrocytes   总被引:1,自引:0,他引:1  
Todorich B  Zhang X  Connor JR 《Glia》2011,59(6):927-935
There is a critical relationship between oligodendrocyte development, myelin production, and iron bioavailability. Iron deficiency leads to hypomyelination both in humans and animal models, and the neurological sequelae of hypomyelination are significant. Therefore, understanding molecular mechanisms of iron import into oligodendrocytes is necessary for devising effective strategies for iron supplementation. Although transferrin has been considered as an essential component of oligodendrocyte media in culture, oligodendrocytes in vivo lack transferrin receptors. We have established that receptors for H-ferritin (HF) exist on cells of oligodendroglial lineage and that uptake of extracellular HF by oligodendrocyte progenitors is via receptor mediated endocytosis. These data strongly argue that ferritin is a major source of iron for oligodendrocytes. In this study, we demonstrate that media deficient in transferrin results in loss of viability of oligodendrocyte progenitors in culture. Cell loss could be prevented by supplementing the media with HF. Moreover, the addition of extracellular HF stimulates development of oligodendrocyte progenitor cells (OPCs) by increasing expression of myelin basic protein (MBP) and olig2 proteins without increasing their proliferation. The effect of HF on the OPCs could be mimicked by addition of membrane permeable 3,5,5-trimethylhexanoyl ferrocene (TMH-Fe) as an iron source to the media, but not membrane-impermeable ferric ammonium citrate. Overall, therefore, our results demonstrate the importance of iron for OPCs viability and differentiation and identify extracellular HF as a critical source of iron for oligodendrocytes. Given that ferritin receptors, but not transferrin receptors can be demonstrated on oligodendrocytes in vivo, the delivery of iron to oligodendrocytes via ferritin may be the more biological relevant delivery system.  相似文献
4.
Our aim was to investigate the relation between behavioral symptoms and hematological variables which are related with iron deficiency and anemia, ferritin, hemoglobin, mean corpuscular volume (MCV), and reticulosite distribution width (RDW) in children and adolescents with pure Attention Deficit Hyperactivity Disorder (ADHD) or ADHD comorbid with other psychiatric disorders. The sample consisted of 151 subjects with ADHD, 45 of these subjects had other comorbid conditions. Conners Parent (CPRS) and Teacher Rating Scales (CTRS) were obtained. Comorbid ADHD subjects had lower mean hemoglogin and MCV. In the ADHD group in general, CPRS and CTRS Total scores were significantly negatively correlated with ferritin level. When only pure ADHD subjects were taken into account, the correlations did not reach statistical signifance. Overall, these results suggested that lower ferritin level was associated with higher behavioral problems reported by both parents and teachers. Presence of comorbid conditions might increase the effect of lower iron stores on behavioral measures.  相似文献
5.
脑缺血对大鼠循环铁的影响   总被引:1,自引:0,他引:1  
目的 探讨脑缺血对大鼠循环铁的影响。方法 雄性Wistar大鼠随机分为脑缺血1、3、7、28d和假手术组;脑缺血组结扎双侧颈总动脉造成大鼠脑缺血,假手术组仅分离出双侧颈总动脉但不结扎;采用比色法测定大鼠血清铁含量;用放射免疫法测定血清铁蛋白含量。结果脑缺血1d大鼠血清铁含量低于假手术组(P〈0.05);缺血3d血清铁含量虽低于假手术组,但无统计学意义(P〉0.05);之后随着缺血时间的延长血清铁含量呈增高趋势,缺血28d时显著高于假手术组(P〈0.01)。脑缺血1d大鼠血清铁蛋白含量与假手术组比较无明显差别(P〉0.05);缺血3d组低于假手术组(P〈0.05);之后随着缺血时间的延长血清铁蛋白含量呈增高趋势,在缺血7、28d时均高于假手术组(P〈0.05)。结论脑缺血早期引起大鼠循环铁降低,晚期升高;循环铁的改变可能参与了脑铁含量升高和神经元铁沉积过程。  相似文献
6.
OBJECTIVE: Increasing evidence suggests a significant comorbidity between attention-deficit/hyperactivity disorder (ADHD) and restless legs syndrome (RLS). Iron deficiency may underlie common pathophysiological mechanisms in subjects with ADHD plus RLS (ADHD+RLS). To date, the impact of iron deficiency, RLS and familial history of RLS on ADHD severity has been scarcely examined in children. These issues are addressed in the present study. METHODS: Serum ferritin levels, familial history of RLS (diagnosed using National Institutes of Health (NIH) criteria) and previous iron supplementation in infancy were assessed in 12 ADHD+RLS children, 10 ADHD children and 10 controls. RLS was diagnosed using NIH-specific pediatric criteria, and ADHD severity was assessed using the Conners' Parent Rating scale. RESULTS: ADHD symptom severity was higher, although not significantly, in children with ADHD+RLS compared to ADHD. The mean serum ferritin levels were significantly lower in children with ADHD than in the control group (p<0.0005). There was a trend for lower ferritin levels in ADHD+RLS subjects versus ADHD. Both a positive family history of RLS and previous iron supplementation in infancy were associated with more severe ADHD scores. CONCLUSIONS: Children with ADHD and a positive family history of RLS appear to represent a subgroup particularly at risk for severe ADHD symptoms. Iron deficiency may contribute to the severity of symptoms. We suggest that clinicians consider assessing children with ADHD for RLS, a family history of RLS, and iron deficiency.  相似文献
7.
Summary Histotoxic hypoxia was experimentally produced in the rat brain by sodium azide intoxication. In spite of the absence of light microscopic alterations, electron micrographs showed the early changes in the cerebral cortex and subcortex which consisted of widening of the intercellular space and swelling of the pericapillary astrocytic end-feet. However, ferritin-albumin mixture, used for the tracer of protein transport, did not show any increase in the capillary permeability. Sodium azide was considered to disturb the water metabolism, producing osmotic unbalance in the brain.
Zusammenfassung Experimentelle histotoxische Hypoxämie wurde in Ratten durch Natriumacid-Vergiftung erzeugt. Die mikroskopische Untersuchung der Hirne ergab keine Veränderung, aber bei der elektronenmikroskopischen Untersuchung fanden sich als Frühveränderungen in der Hirnrinde und im Subcortex eine Erweiterung der intercellulären Räume und eine Anschwellung der pericapillären Astrocytenfortsätze. Ferritinalbumin-Mischung, die als Indicator des Eiweißtransportes benutzt wurde, zeigte keine Vermehrung der Capillarpermeabilität. Es wird vermutet, daß Natriumacid den Wasserstoffwechsel beeinträchtigt, wobei es eine osmotische Ausgleichstörung im Gehirn erzeugt.
  相似文献
8.
Summary Small quantities of ferritin or Thorotrast were injected into the visual cortex of the cat in order to determine which cells in the cerebrum exhibited endocytic activity when exposed to foreign material. Blocks of tissue from the cortex and adjacent white matter were examined by electron microscopy 5 h and 20 h after injection.Some neurons and oligodendrocytes and all the astrocytes examined showed pinocytotic activity. Increasing the time of exposure of the tissue to the tracer did not appear to result in greatly increased uptake by individual cells, except in the case of astrocytes.Neutrophils, which invaded the tissue after 5 h, and in increased numbers after 20 h, exhibited minimal endocytic activity when they invaded the grey matter. They were more active in the white matter.Up to 20 h after injection, the three most important endocytic elements in the cerebrum were astrocytes (collectively), pale perivascular cells within the basement membrane of the blood vessels and phagocytes, which appeared to be reactive endogenous cells.Many thanks are due to Professor M. J. Blunt, School of Anatomy, University of N.S.W., for his helpful criticisms throughout the course of this study, which was supported by a research grant from the National Health and Medical Research Council of Australia to Professor Blunt. The assistance of Mrs. Ruth Mather is gratefully acknowledged.  相似文献
9.
Summary Human glioblastoma cells in long-term monolayer culture showed an even distribution of intramembrane particles (IMP) on all surfaces of the plasma membrane; junctional complexes were rearely observed and rectilinear arrays were not seen. Cells treated with Con A-ferritin an dRicin II-ferritin showed an even distribution of lectin receptors and under conditions used no capping occurred. Lectin-ferritin complexes were taken up into pinocytotic vesicles. Cleaved preparations of Ricin II-ferritin treated cells showed no change in the distribution of IMP.The authors wish to thank Dr. E. E. Manuelidis for invaluable advice, J. P. Albert and P. Johnson for expert technical assistance, and Mrs. M. Silberberg for excellent secretarial work.This work was supported by NIH Grants GM 02140-05 and CA 15044-03 and USPHS 5 P01 NS-06208-11.  相似文献
10.
Summary Mice injected intrapertoneally with 20 mg of ferritin twice weekly for more than 4 weeks developed proteinuria due to the deposition of ferritin-antiferritin antibody complexes in renal glomeruli. Deposits of ferritin, immunoglobulin G (IgG) and third complement component (C3) also accumulated in the perivascular, extracellular space of the choroid plexus as demonstrated by immunofluorescence and electron microscopy. The findings confirm previous observations on immune complex deposits in the choroid plexus in spontaneous autoimmune disease and persistent viral infections. The occurrence of similar deposits in the human choroid plexus and the possibility of an associated disturbance of the blood-spinal fluid barrier are discussed.Supported by National Institutes of Health Grant NS 11739  相似文献
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