Changes in intestinal microbiome and barrier function are critical in the development of alcohol-related liver disease (ALD). Here, we determined the effects of a one-week alcohol withdrawal on parameters of intestinal barrier function in heavy drinkers with ALD in comparison to healthy non-drinkers (controls). In serum samples of 17 controls (m = 10/f = 7) and 37 age-matched ALD patients (m = 26/f = 11) undergoing a one-week alcohol withdrawal, markers of liver health and intestinal barrier function were assessed. Liver damage, e.g., fibrosis and hepatic steatosis, were assessed using FibroScan. Before alcohol withdrawal, markers of liver damage, lipopolysaccharide binding protein (LBP) and overall TLR4/TLR2 ligands in serum were significantly higher in ALD patients than in controls, whereas intestinal fatty acid binding protein (I-FABP) and zonulin protein concentrations in serum were lower. All parameters, with the exception of LBP, were significantly improved after alcohol withdrawal; however, not to the level of controls. Our data suggest that one-week of abstinence improves markers of intestinal barrier function and liver health in ALD patients. 相似文献
Background: An understanding of the factors that influence intestinal wall integrity during the transition from fetal to neonatal life is important to elucidate and minimize potential contributions to the development of intestinal pathologies in vulnerable newborns.
Objective: To assess the factors affecting intestinal wall integrity of late preterm and term newborns at birth.
Methods: The concentrations of serum intestinal fatty acid-binding protein (I-FABP) as biomarker of intestinal wall injury, superoxide dismutase (SOD), and glutathione peroxidase (Gpx) as parameters of oxidative stress, and blood gas analyses were measured in the arterial cord blood of 80 newborns, delivered by spontaneous vaginal delivery (labor group) or elective cesarean delivery (non-labor group).
Results: Compared with the non-labor group, I-FABP, SOD, and Gpx levels were significantly higher in the labor group. I-FABP concentration was inversely correlated with pH and BE and positively correlated with SOD and Gpx concentrations, second stage of labor duration, and active pushing time.
Conclusions: This study demonstrates that oxidative stress at birth and prolonged second stage of labor may lead to the intestinal injury, which is reflected in increased serum concentration of I-FABP. 相似文献
In recent years several potential biochemical markers have been evaluated to facilitate a reliable diagnosis of necrotizing enterocolitis (NEC), but none have made progress to clinical routine. We performed a comparative assessment in premature infants to evaluate the diagnostic value of the routinely available cytokine interleukin (IL)-8, and two promising experimental biomarkers, the gut barrier proteins liver fatty acid binding protein (L-FABP) and intestinal fatty acid binding protein (I-FABP), respectively, for the diagnosis of NEC.
Methods
IL-8, L-FABP, and I-FABP concentrations were analyzed in the serum of 15 infants with NEC and compared with 14 gestational age-matched infants serving as a control group.
Results
Serum concentrations of I-FABP, L-FABP and IL-8 were significantly higher in infants with NEC compared with controls. IL-8 showed the highest diagnostic value with an area under the curve of 0.99, followed by L-FABP and I-FABP. In addition we found a significant correlation between IL-8 and both FABPs in infants with NEC.
Conclusion
Our results further advocate the possible role of IL-8 as a specific marker for NEC. The diagnostic value of IL-8 seems to be superior to I-FABP, and similar to L-FABP. The routinely availability facilitates IL-8 as a possible candidate for further clinical investigations. 相似文献
Serum Intestinal Fatty-Acid Binding Protein (I-FABP) is a useful marker of bowel necrosis in pediatric intussusception. The aim of this study is to determine the sensitivity of this marker and correlate it with length of necrosed small bowel.
Methods
A single-centre prospective study of 50 children presenting to Lagos University Teaching Hospital, Nigeria, in whom a diagnosis of intussusception was made over 1 year was completed. Additionally, 25 age- and sex-matched controls (day case surgery) were recruited. They were grouped into three: 25 children with necrotic bowel, 25 without bowel necrosis, and 25 controls. The serum IFABP levels were compared between the cohorts with confirmed bowel necrosis at surgery and those with no necrosis, as well as controls. The cut-off values for the diagnosis of bowel necrosis were calculated using a receiver operating characteristic curve (ROC). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated.
Results
Twenty-five children were diagnosed with necrotic intussusception whose serum IFABP immunoassay has significantly higher median compared with those without necrosis and controls (2056.0 ng/ml vs. 943.0 ng/ml and 478.0 ng/ml P = 0.0002). Using a cut-off value of 1538 ng/ml, the sensitivity, specificity, PPV, and NPV were 64%, 88%, 84%, and 71%, respectively. I-FABP titer greater than 1538 ng/ml was found to have higher likelihood of necrotic bowel (p = 0.002; odds ratio 13.04; 95% confidence interval; 0.618–0.891).
Conclusion
Serum I-FABP is moderately sensitive for discriminating between bowel necrosis, and it predicts increased likelihood of bowel resectability in intussusception.
Level of evidence
Level II – Development of diagnostic criteria in a consecutive series of patients and a universally applied “gold standard”. 相似文献
ObjectivesThe study aimed to examine if the α-diversity and relative abundance of the gastrointestinal bacterial taxa is associated with the response magnitude of markers characteristic of exercise-induced gastrointestinal syndrome in response to exertional-heat stress.DesignCross-sectional.MethodsTwenty-two endurance-trained athletes completed 2 h running at 60% O2max in hot ambient conditions (35.2 °C, 25% relative humidity). Faecal samples were collected pre-exercise to determine bacterial taxonomy by 16S rRNA amplicon sequencing (Illumina MiSeq platform). Data were processed using the QIIME2 pipeline (v2019.1) establishing taxonomic classification with >95% confidence using SILVA. Pre- and post-exercise blood samples were used to determine plasma I-FABP and cortisol concentrations, and systemic inflammatory response profile. Markers of physiological and thermoregulatory strain, and gastrointestinal symptoms were measured every 10 min during exercise. Associations were determined by partial correlation controlled for body mass variables.ResultsPositive associations between Tenericutes (r(18) = 0.446, p = 0.049) and Verrucomicrobia (r(18) = 0.450, p = 0.046) phylum, Akkermansiaceae (r(18) = 0.486, p = 0.030) and Ruminococcaceae (r(18) = 0.449, p = 0.047) family and aligned genus groups with I-FABP were observed. Whilst, associations between Faecalibacterium (r(12) = 0.668, p = 0.009) and Ruminoclostridium-9 (r(12) = −0.577, p = 0.031) genus with systemic inflammatory profile were observed. Association between bacterial phyla, family, and genus groups were also observed for gastrointestinal symptoms and markers of thermoregulatory strain (r(18) >0.400, p < 0.05).ConclusionsThe relative abundance of several commensal bacterial groups showed modest favourable (i.e., low perturbations) or detrimental associations with the magnitude of gastrointestinal integrity perturbations and symptoms, and potentially influences body temperature change, in response to exertional-heat stress. 相似文献
Treatment of mice with high sublethal doses of cholera entero-exotoxin, or chol-eragen, given i.v. on days 4 and 6 post-coitum, i.e., around the time of nidation, decreases or suppresses pregnancy in females of 3 different strains of mice. Our studies reveal that the toxin interferes with embryonic growth and development after day 7 post-coitum, producing complete embryo resorption sometime between days 9 and 11 post-coitum.Studies of plasma progesterone reveal normal or slightly elevated levels on day 7 post-coitum and a marked decrease on day 9 post-coitum in toxin-treated mice, indicating that failure of the corpus luteum occurs sometime between these two days. Exogenous progesterone during this period partially or totally prevents the abortifo-cient effect of the toxin, at least until day 9 post-coitumIn the period preceding abortion, treated mice may show a slight increase in frequency of pregnancy or number of implants per mouse, probably related to an initial but transitory stimulation of the corpus luteum by the toxin. A similar tendency was observed in mice treated with low doses of this substance by i.p. injection, suggesting that low doses of toxin might improve reproductive performance. 相似文献