全文获取类型
收费全文 | 378篇 |
免费 | 25篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 4篇 |
妇产科学 | 9篇 |
基础医学 | 52篇 |
口腔科学 | 8篇 |
临床医学 | 15篇 |
内科学 | 33篇 |
皮肤病学 | 10篇 |
神经病学 | 10篇 |
特种医学 | 2篇 |
外科学 | 28篇 |
综合类 | 37篇 |
预防医学 | 47篇 |
眼科学 | 9篇 |
药学 | 90篇 |
中国医学 | 31篇 |
肿瘤学 | 25篇 |
出版年
2024年 | 1篇 |
2022年 | 2篇 |
2021年 | 19篇 |
2020年 | 7篇 |
2019年 | 7篇 |
2018年 | 10篇 |
2017年 | 9篇 |
2016年 | 8篇 |
2015年 | 14篇 |
2014年 | 18篇 |
2013年 | 21篇 |
2012年 | 14篇 |
2011年 | 20篇 |
2010年 | 19篇 |
2009年 | 11篇 |
2008年 | 22篇 |
2007年 | 17篇 |
2006年 | 19篇 |
2005年 | 16篇 |
2004年 | 14篇 |
2003年 | 11篇 |
2002年 | 10篇 |
2001年 | 9篇 |
2000年 | 9篇 |
1999年 | 11篇 |
1998年 | 9篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 7篇 |
1994年 | 3篇 |
1993年 | 8篇 |
1992年 | 8篇 |
1991年 | 5篇 |
1990年 | 4篇 |
1989年 | 7篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 5篇 |
1985年 | 8篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 1篇 |
排序方式: 共有413条查询结果,搜索用时 296 毫秒
1.
目的:观察理气中药经验组方对糖尿病大鼠胃排空延迟的干预作用。方法:雌雄各半成年SD大鼠110只,随机分为正常组(A组)、糖尿病中药组(B组)、糖尿病胃复安组(C组)、糖尿病组(D组)。A组、D组1次/d按10 mL/只予0.9%生理盐水灌胃,B组1次/d按8 mL/只予中药煎剂灌胃,C组1次/d按0.5 mg/只予胃复安片灌胃。喂养12周后,行13C胃排空实验及甲基橙水溶液胃排空实验,观察各组大鼠胃排空情况。结果:糖尿病大鼠胃排空较正常大鼠明显延迟(P0.01),糖尿病大鼠胃排空延迟模型制作成功。糖尿病中药组和糖尿病胃复安组大鼠胃排空较糖尿病组快(P0.01);糖尿病中药组大鼠胃排空较糖尿病胃复安组大鼠快(P0.05)。结论:常规喂养12周后糖尿病大鼠出现胃排空延迟。理气中药陈皮、枳实、木香、香附组方煎剂和胃复安均能促进糖尿病大鼠胃排空,理气中药组方煎剂的效果优于胃复安。 相似文献
2.
Wataru Kimura 《Journal of hepato-biliary-pancreatic sciences》2003,10(2):156-162
The differences and similarities between intraductal papillary mucinous tumor (IPMT) and mucinous cystadenoma or carcinoma (mucinous cystic tumor; MCT) of the pancreas have been noted. The similarities include: (1) both tumors originate from pancreatic duct cells, (2) massive mucin production is found in both tumors, and (3) papillary projection is a common histological characteristic. However, there are also many differences. IPMT is most frequently found in men in their sixties, and originates in the head of the pancreas, with 62% (123/199) of tumors reported to be found in the head of the pancreas. This tumor sometimes spreads throughout the entire pancreas. The tumor itself basically is of the dilated pancreatic duct type, and the prognosis is generally good. In contrast, MCT frequently develops in women in their forties. This tumor is usually large, round, and almost totally encapsulated by fibrous tissue, with no communication with the pancreatic duct. The tumor histologically has an ovarian-like stroma. It most often develops in the body or tail of the pancreas. Invasion is often present and the operative prognosis is not good. IPMT resembles the shape of a bunch of grapes and MCT resembles that of an orange. From the differences between these two types of tumors, they are classified into different categories. With regard to therapeutic strategies for MCT, the tumor should be resected with lymph node dissection immediately when it is detected. In contrast, some patients with branch-type IPMT can be followed without surgical procedures. Because IPMT shows good prognosis and little tendency for infiltration, some kinds of organ-preserving procedures would be possible for some patients with this tumor. Such organ-preserving procedures are: duodenum-preserving pancreas head resection, spleen-preserving distal pancreatectomy with conservation of the splenic artery and vein, and so on. 相似文献
3.
目的通过了解睾丸支持细胞(Sertoli cells,SC)的基本生物学特性,探讨SC鉴定的良好方法。方法联合应用复合胶原酶及差速贴壁法从睾丸组织中分离、培养SC,光镜和电镜下观察细胞的形态、MTT法测定细胞的生长曲线,观察其在体外培养条件下的增殖特性;利用免疫细胞染色及免疫荧光染色的方法,检测Fas配体(FasL)的表达,观察其免疫功能;应用吖啶橙荧光染色进行细胞鉴定。结果联合应用复合胶原酶及差速贴壁法分离、培养的细胞在光镜下呈长柱状及三角形,增殖能力强,电镜下胞核中可见特异性卫星小体,胞质中细胞器丰富,免疫细胞染色及免疫荧光染色证实其高表达FasL,吖啶橙荧光染色可见胞核中含有大量异染色质,核仁明显,证实其为SC。结论复合胶原酶及差速贴壁法分离的SC具有良好的增殖能力及免疫功能,电镜、吖啶橙荧光染色是鉴定SC方便、有效的方法。 相似文献
4.
本文报告了葡萄糖酸锌糖浆的制法及用二甲橙指示剂法测定含量的方法。结果表明制法简便,含量测定方法准确。 相似文献
5.
暗柳橙(Citrus sinensis Osbeck)第一次生理落果期(3月下旬~4月中旬)的果实外植体脱落过程中,离区纤维素酶A、B的活性明显升高,第二次落果(4月下旬~5月中旬)期的果实脱落过程中,纤维素酶A的活性也显著提高,但纤维素酶B活性变化较小。细胞内、外纤维素酶的活性在外植体脱落过程中也不断升高。2,4—D能明显抑制胞内、外纤维素酶及纤维素酶A的活性,同时还能抑制外植体及非离休果实内乙烯的产生,但对纤维素酶B活性的影响无明显效应。田间喷布低浓度的2,4—D,明显地减少第一次生理落果,但对第二次落果的作用不如赤霉素。本文还讨论了果实的脱落、纤维素酶活性与乙烯之间的相互关系,及2,4—D的调控作用。并认为采用外植体的方法不失为研究脱落问题的有效手段。 相似文献
6.
E. M. Ryazanov A. I. Filatov N. N. Zybina Yu. A. Mazing G. S. Alekseev M. Yu. Solov'ev 《Bulletin of experimental biology and medicine》1990,110(6):1651-1653
(Presented by Academician of the Academy of Medical Sciences of the USSR A. N. Klimov.) Translated from Byulleten' Éksperimental'noi i Biologii i Meditsiny, Vol. 110, No. 12, pp. 604–606, December, 1990. 相似文献
7.
Aida Salihagić Marija Mačković Hrvoje Banfić Ivan Sabolić 《Pflügers Archiv : European journal of physiology》1988,413(2):190-196
The effect of unilateral nephrectomy on Na+–H+ exchange in rat renal cortical brush-border membrane vesicles (BBMV) was studied by the method of acridine orange fluorescence quenching. The exchanger activity in BBMV from remnant kidney increased rapidly by 70–75% within first 30 min following uninephrectomy. Only a slight further increase was found in later stages of renal growth, i.e. 30 min to 7 days following uninephrectomy. The changes in antiporter activity were restricted toV
max, whereas theK
m for Na+ was similar in control and compensatory growing kidney. The increase of Na+–H+ exchange at 15 min was not affected by actinomycin D in vivo, whereas the increase at 48 h was completely abolished indicating that protein synthesis could be involved in the late, but not in the initial stimulation of renal Na+–H+ exchange. The late, but not the initial stimulations of Na+–H+ exchange were associated with elevated activities of cortical (Na++K+)-ATPase indicating that changes in antiporter activity precede those in the (Na++K+)-pump. The early stimulation of Na+–H+ exchange in BBMV in one kidney was induced also by the occlusion of blood flow through the contralateral kidney for 15 min, without removing it. Thirty min after the occlusion was removed and the reflow established, the Na+–H+ exchange in BBMV from the intact kidney decreased to the control values. The observed modulations in renal Na+–H+ exchanger may be regulated by phosphorylation-dephosphorylation events. In support, the concentration of a well known protein kinase C activator, 1,2-diacylglycerol, in the cortical tissue of the remnant kidney increased up to 100% within 5 min following unilateral nephrectomy and preceded the increase in Na+–H+ exchange. The early stimulation of Na+–H+ exchange may be a trigger in initiating the kidney growth. 相似文献
8.
K. N. Fedorova 《Bulletin of experimental biology and medicine》1976,82(1):1093-1095
A luminescence-microscopic investigation of short-term human cell cultures stained with Acridine Orange showed that the melting curve of the cell chromatin DNA in the region from 78 to 85°C depends on changing external environmental conditions, i.e., on the composition of the blood serum.Presented by Academician of the Academy of Medical Sciences of the USSR N. A. Yudaev.Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 82, No. 7, pp. 878–879, July, 1976. 相似文献
9.
R. Fuchs T. Thalhammer M. Peterlik J. Graf 《Pflügers Archiv : European journal of physiology》1986,406(4):430-432
Mechanisms of Na+–H+ exchange in the hepatocyte were studied utilizing isolated basolateral membrane vesicles prepared by two different methods: Evidence was obtained for the existence of molecular coupling of Na+ and H+ fluxes (Na+/H+-antiport) which exhibits saturation kinetics (Km 7 mmol/l Na+) and is inhibited by amiloride (1.0 mmol/l). Although the two membrane preparations showed differences with respect to ionic permeabilities, our data suggest that a relatively high H+ conductance exists in the basolateral plasma membrane. Hence, electrical coupling of conductive H+ and Na+ fluxes in the opposite direction could contribute to net Na+–H+ exchange across the basolateral hepatocyte plasma membrane. 相似文献
10.