Safety and feasibility of laparoscopic living donor right hepatectomy for adult liver transplantation: a meta-analysis

BackgroundLaparoscopic living donor right hepatectomy (LDRH) was a controversial topic due to its unknown safety and feasibility.MethodsPubMed, EMBASE and Cochrane Library databases were searched for studies comparing LDRH with open living donor right hepatectomy (ODRH), which were published between the date of database establishment and June 2020. Revman5.3 was used for statistical analysis.ResultsFourteen studies were included. For the donors, there was no significant difference in warm ischemic time, hospital stay, graft weight, hepatic arterial anomalies (HAA), hepatic vein anomalies (HVA), portal vein anomalies (PVA), biliary anomalies, bleeding, wound infection, severe complication rate and readmission rate. The estimated blood loss, incidence of complication, intra-abdominal fluid rate in the LDRH group were significantly lower than those in the ODRH group, while the operation time, time to remove liver in the LDRH group were significantly higher than those in the ODRH group. For the recipients, there was no significant difference in complication rate, bleeding, HAA, PVA, biliary anomalies, graft failure and mortality. The HVA rate in the LDRH group was significantly higher than that in the ODRH group.ConclusionLDRH is safe and feasible for adult living donor liver transplantation compared with ODRH and it can reduce intraoperative bleeding and postoperative complication in donors, which requires further verification by more multi-center comparative studies with large sample and high quality.     

新辅助化疗后门静脉系统全置换治疗局部进展期胰腺癌一例报告

患者女性,65岁,局部进展期胰腺癌伴门静脉、肠系膜上静脉和肠系膜上动脉侵犯,经6个周期奥沙利铂+氟尿嘧啶+伊立替康+亚叶酸钙(FOLFIRINOX)新辅助化疗后,肿瘤评估为部分缓解,但仍属于局部进展期胰腺癌。患者行动脉优先入路、全门静脉系统切除、异体血管置换重建的扩大胰十二指肠切除术,同时运用异体血管体外成形技术。患者术后已无瘤生存28个月。FOLFIRINOX新辅助化疗方案可使部分局部进展期胰腺癌患者疾病达到稳定状态,而异体血管体外成形技术的应用为门静脉系统全切除置换提供了一种新的方法,使患者有机会达到根治性手术切除,从而改善远期预后。     

Intrawound application of vancomycin reduces the proportion of fracture-related infections in high-risk tibial plateau fractures

IntroductionDespite the improvements in surgical techniques and the use of prophylactic intravenous antibiotics, the fracture-related infection (FRI) incidence after high-risk tibial plateau fractures remains high. This study aimed to evaluate the clinical effect of the intrawound application of vancomycin on the FRI after high-risk tibial plateau fracture surgery.MethodsA total of 243 patients who underwent high-risk tibial plateau fracture surgery from May 2013 to June 2021 were retrospectively reviewed. Of these, 233 cases were enrolled. Considering the preoperative patient condition, surgeons applied vancomycin powder directly into the surgical site before wound closure in 105 cases (intrawound application of vancomycin powder with preoperative intravenous cephalosporin). The remaining 128 cases served as the control group (preoperative intravenous cephalosporin alone). Clinical data and surgical details were recorded. The Cox proportional hazards regression analysis was used to assess risk factors for FRI. The Kaplan-Meier method and the log rank test illustrated the infection status of patients based on the application of intrawound vancomycin. The primary outcome was an FRI within one year. Secondary outcomes included bacterial culture and vancomycin-related complications.ResultsOur study demonstrated a significant difference in the incidence of FRI between the vancomycin group and the control group (3.8% versus 10.9%; p=0.041). Multivariable Cox regression showed the intrawound application of vancomycin powder decreased the rate of FRI. There were no complications related to intrawound vancomycin observed during follow-up. The presence of Gram-positive FRI was higher in the control group compared with the vancomycin group.ConclusionsIntrawound application of vancomycin was associated with a significant lower rate of FRI after high-risk tibial plateau fracture surgery compared to the control group.     

自制静脉止血带在儿童腹腔镜疝修补手术中的应用

目的探讨自制静脉止血带在儿童腹腔镜疝修补手术中的应用价值及护理经验。 方法选择2017年1月至2018年12月，首都医科大学附属北京朝阳医院行儿童腹腔镜疝修补手术224例患儿的临床资料。将2017年1至12月行手术治疗的115例患儿设置为对照组，使用传统圆形止血带进行静脉穿刺。2018年1至12月行手术治疗的109例患儿设置为观察组，使用自制静脉止血带进行静脉穿刺。比较2组穿刺疼痛度及穿刺成功率。 结果观察组在减轻静脉穿刺疼痛度，提高静脉穿刺成功率方面与对照组比较，差异有统计学意义（P<0.05）。 结论自制止血带的应用可以减轻儿童腹腔镜疝修补手术静脉穿刺疼痛度提高静脉输液成功率。     

Profilin 1, negatively regulated by microRNA-19a-3p,serves as a tumor suppressor in human hepatocellular carcinoma

Profilin 1 (PFN1) is a critical actin-regulatory protein; however, its functional role in hepatocellular carcinoma (HCC) progression remains to be further elucidated. In the present study, we observed that the expression levels of PFN1 were significantly decreased in HCC tissues and cell lines. Low PFN1 expression was significantly correlated with aggressive clinicopathological characteristics and poor prognosis of HCC patients. Further in vitro experiments demonstrated that overexpression of PFN1 remarkably inhibited the proliferation, migration, invasion and EMT of HCC cells. Moreover, we also found that PFN1 was a direct target gene of miR-19a-3p, and in HCC tissues, and there was a significantly inverse correlation between PFN1 mRNA and miR-19a-3p expression. Collectively, our results showed that PFN1 functions as a tumor suppressor in HCC, and might serve as a diagnostic and therapeutic target for HCC patients.     

Supersonic Shear Imaging for Quantification of Lateral Abdominal Muscle Shear Modulus in Pediatric Population with Scoliosis: A Reliability and Agreement Study

The aim of this study was to assess the intra- and inter-rater reliability/agreement of the lateral abdominal muscle (LAM) stiffness and thickness measurements at rest and during contraction and to determine the relationship between the superficial fat thickness and the LAM stiffness measurements. LAM stiffness and thickness were measured using supersonic shear wave elastography (SSI) in pediatric participants. The reliability of LAM stiffness and thickness measurements ranged from moderate to excellent. There was an inverse correlation between fat thickness and between-rater difference in the resting external oblique stiffness (r > –0.37) and the contracted external and internal oblique stiffness (r > –0.40). SSI is a reliable method for assessing LAM stiffness and thickness in pediatric populations. To remove potential systematic errors: (i) the first round of measurements should be performed to familiarize patients with procedures; (ii) the examiner should pay more attention while performing LAM measurements on the opposite side of the body.